Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice

Wei Jiang; Song Li; Wei Mao; Guang Yang; Xin-mei Li; Guang-juan Zheng; Huan-lin Wu; Xin-min Ruan; Ke-ji Chen

doi:10.1007/s11655-012-1123-x

Your Location：

Home >

Browse articles >

Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice

OriginalPaper | Updated：2021-08-27

- Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice
- Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice
- 中国结合医学杂志（英文版） 2012年18卷第6期页码：451-456
- Affiliations：
  
  1. The Second Affiliated Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
  2. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81102584) and Guangdong Provincial Administration of Traditional Chinese Medicine (No. 2009386)
- DOI：10.1007/s11655-012-1123-x
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-7-22，
Scan for full text
Jiang, W., Li, S., Mao, W. et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice., Chin. J. Integr. Med. 18, 451–456 (2012). https://doi.org/10.1007/s11655-012-1123-x

Wei Jiang, Song Li, Wei Mao, et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice[J]. Chinese Journal of Integrative Medicine, 2012,18(6):451-456.
Jiang, W., Li, S., Mao, W. et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice., Chin. J. Integr. Med. 18, 451–456 (2012). https://doi.org/10.1007/s11655-012-1123-x DOI：

Wei Jiang, Song Li, Wei Mao, et al. Effect of Huxin Formula (护心方) on reverse cholesterol transport in ApoE-gene knockout mice[J]. Chinese Journal of Integrative Medicine, 2012,18(6):451-456. DOI： 10.1007/s11655-012-1123-x.

摘要

To observe the effect of Huxin Formula (护心方

HXF) on expressions of the chief reverse cholesterol transport (RCT) associated genes

caveolin-1 and scavenger receptor-BI (SR-BI) in ApoE-gene knockout [ApoE (−/−)] mice. Thirty ApoE (−/−) mice of 4–6 weeks old were randomly divided into three groups (A-C). After being fed with high-fat diet for 16 weeks

they were treated with HXF (1 mL/100 g)

pravachol (0.3 mg/100 g)

and saline in equal volume respectively for 16 weeks successively; in addition

a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment

their paraffin sections of aortic tissue were used to measure the size of plaque

expressions of cavolin-1 and SR-BI were detected by immunological histochemical method. As compared with the blank group

levels of caveolin-1 and SR-BI were increased in Groups A and B (P<0.01); but the increase in Group A was more significant than that in Group B (P<0.05). The plaque/aorta area ratio decreased significantly in Groups A and B

but showed insignificant difference between the two groups. HXF could obviously increase the expressions of RCT associated genes

caveolin-1 and SR-BI

promote the RCT process

so as to reduce the formation of aorta atherosclerotic plaque in ApoE (−/−) mice.

Abstract

To observe the effect of Huxin Formula (护心方

HXF) on expressions of the chief reverse cholesterol transport (RCT) associated genes

they were treated with HXF (1 mL/100 g)

pravachol (0.3 mg/100 g)

and saline in equal volume respectively for 16 weeks successively; in addition

a blank group was set up with 10 C57BL/6J mice of 6-week old received 16-week high-fat feeding and saline treatment. Animals were sacrificed at the termination of the experiment

their paraffin sections of aortic tissue were used to measure the size of plaque

expressions of cavolin-1 and SR-BI were detected by immunological histochemical method. As compared with the blank group

but showed insignificant difference between the two groups. HXF could obviously increase the expressions of RCT associated genes

caveolin-1 and SR-BI

promote the RCT process

so as to reduce the formation of aorta atherosclerotic plaque in ApoE (−/−) mice.

关键词

Huxin formulaApoE-gene knockout micereverse cholesterol transportcaveolin-1scavenger receptor-B I

Keywords

Huxin formulaApoE-gene knockout micereverse cholesterol transportcaveolin-1scavenger receptor-B I

references

Luo QF, Sun L, Du GH. Progress in new drugs targeting reverse cholesterol transport. J Chin Pharmacol Bull (Chin) 2006;22:904–907.

Yang X, Quan ZH. The different ways of cellular cholesterol efflux and the relative importance in reverse cholesterol transport process. Am J Chin Clin Med (Chin) 2005;7:164–166.

Van der Veen JN, Van Dijk TH, Vrins CL, Van Meer H, Havinga R, Bijsterveld K, et al. Activation of the liver X receptor stimulates trans-intestinal excretion of plasma cholesterol. J Biol Chem 2009;284:19211–19219.

Gu XJ, Bernardo T, Shangzhe X, Susan A, Jodie B, Monty K. The efficient cellular uptake of high-density lipoprotein lipids via scavenger receptor class B type I require not only receptor-mediated surface binding but also receptorspecific lipid transfer mediated by its extra-cellular domain. J Biol Chem 1998;273:35388.

Qin L, Qin XP, Zhu BY, Liao DF. Changes of caveolin-1 in development of atherosclerosis in apoE-deficient mice. Chin J Clin Pharmacol Therap (Chin) 2004;9:978–982.

Li SH, Zhang H, Duan CW, Wen XL, Yan PK. The inhibitory effect of oxidized low density lipoprotein on the expression of caveolin-1 in vascular smooth muscle cell and its relation with extracellular-signal regulated kinases 1/2. Chin J Arterioscl (Chin) 2007;15:178–180.

Wang RR, Zhu BY, Yan PK, Liao DF. Role of caveolin-1 in SR-AI mediated formation of foam cells derived from macrophages. J Fourth Milit Med Univ (Chin) 2007;28:425–428.

Lene M, Marita S, Lene KJ, Ali M, Tor G, Trond B. Hepatic scavenger receptor class B, type I is stimulated by peroxisome proliferators-activated receptor γ and hepatocyte nuclear factor 4α. Biochem Biophys Res Commun 2003;305:557–565.

Yi GH, Tang CK, Mo ZC, Yang YZ. PPAR in the initiation and progression of atherosclerosis. Chemist Life (Chin) 2004;24:240–242.

Fang YX, Xiao ZS. The effect of ginsenosides on lipid regulation in rats feed with high cholesterol diet. Bull Hunan Med Univ (Chin) 1995;20:425–426.

Guo JW, Yang M, Zhu JG, Deng ZJ. New progress on pharmacological cardiovascular effects study of Panax Notoginsenoside on the cardiovascular effects. J Mod Food Pharm (Chin) 2007;17(2):1–4.

Li L, Wang HJ, Gai WC. Summary on pharmacological action and application of Pinellia tuberifera in clinic. Inf Tradit Chin Med (Chin) 2006;23(5):38–40.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

暂无数据