Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

Chun-yan Feng; Xiu-rong Huang; Ming-xin Qi; Song-wen Tang; Yan-hong Hu; Sheng Chen; Fa-jie Ke

doi:10.1007/s11655-012-1144-5

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Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells

OriginalPaper | Updated：2021-08-27

- Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells
- Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells
- 中国结合医学杂志（英文版） 2012年18卷第7期页码：529-533
- Affiliations：
  
  1. Fujian University of Traditional Chinese Medicine,Fuzhou,China
  2. Department of Ophthalmology, Second Affiliated Peoples Hospital of Fujian University of Traditional Chinese Medicine,Fuzhou,China
  3. Research Center of Pathophysiology, Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the Foundation of Fujian Province Health Department Fund (No.2009-1-30) and Fujian Provincie Department of Education Issues (No. JA10176)
- DOI：10.1007/s11655-012-1144-5
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2013-3-27，
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Feng, Cy., Huang, Xr., Qi, Mx. et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells., Chin. J. Integr. Med. 18, 529–533 (2012). https://doi.org/10.1007/s11655-012-1144-5

Chun-yan Feng, Xiu-rong Huang, Ming-xin Qi, et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells[J]. Chinese Journal of Integrative Medicine, 2012,18(7):529-533.
Feng, Cy., Huang, Xr., Qi, Mx. et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells., Chin. J. Integr. Med. 18, 529–533 (2012). https://doi.org/10.1007/s11655-012-1144-5 DOI：

Chun-yan Feng, Xiu-rong Huang, Ming-xin Qi, et al. Mitochondrial proteomic analysis of isopsoralen protection against oxidative damage in human lens epithelial cells[J]. Chinese Journal of Integrative Medicine, 2012,18(7):529-533. DOI： 10.1007/s11655-012-1144-5.

摘要

To investigate the protective effects of the natural medicinal monomer isopsoralen (ISR) with estrogenic activity against oxidative damage in human lens epithelial cells B3 (HLE-B3) caused by hydrogen peroxide (H2O2) and to pursue the possible mitochondrial proteomic regularity of the protective effects. HLE-B3 cells were treated with H2O2 (300 μ mol/L)

β-estradiol (E2: 10−8 mol/L) and H2O2

ISR (10−5 mol/L) and H2O2

or left untreated. Altered expressions of all mitochondrial proteins were analyzed by protein array and surfaceenhanced laser desorption ionization time of flight mass spectrometry (SELDI-TOF-MS). The mass/charge (m/z) ratios of each peak were tested by the Kruskal-Wallis rank sum test

and the protein peak value of the m/z ratio for each treatment by pair comparison was analyzed with the Nemenyi test. H2O2 up-regulated the expressions of two protein spots (with m/z of 6532 and 6809). E2 mitigated the oxidative damage

and the expression of one protein spot (m/z 6532) was down-regulated. In contrast

ISR down-regulated both of protein spots (m/z 6532 and 6809). ISR could effectively inhibit H2O2-induced oxidative damage in HLE-B3 cells. The protein spot at m/z of 6532 might be the target spot of ISR against oxidative damage induced by H2O2.

Abstract

β-estradiol (E2: 10−8 mol/L) and H2O2

ISR (10−5 mol/L) and H2O2

and the expression of one protein spot (m/z 6532) was down-regulated. In contrast

关键词

isopsoralenmitochondrial proteomicslens epithelial cellsenile cataract

Keywords

isopsoralenmitochondrial proteomicslens epithelial cellsenile cataract

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