Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats

Li-li Tao; Yan Lei; Guo-li Wang; Ling-qun Zhu; Yang Wang

doi:10.1007/s11655-012-1180-1

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Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats

OriginalPaper | Updated：2021-08-27

- Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats
- Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats
- 中国结合医学杂志（英文版） 2012年18卷第8期页码：582-590
- Affiliations：
  
  1. Xiyuan Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  2. Research Department, China Academy of Chinese Medical Sciences,Beijing,China
  3. Dongzhimen Hospital, Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Basic Research Program of China (Program 937, No. 2007CB507400), the National Natural Science Foundation of China (No. 30973828), the Independent Topic Program of China Academy of Chinese Medical Sciences (No. Z02151)
- DOI：10.1007/s11655-012-1180-1
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-8-2，
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Tao, Ll., Lei, Y., Wang, Gl. et al. Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats., Chin. J. Integr. Med. 18, 582–590 (2012). https://doi.org/10.1007/s11655-012-1180-1

Li-li Tao, Yan Lei, Guo-li Wang, et al. Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats[J]. Chinese Journal of Integrative Medicine, 2012,18(8):582-590.
Tao, Ll., Lei, Y., Wang, Gl. et al. Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats., Chin. J. Integr. Med. 18, 582–590 (2012). https://doi.org/10.1007/s11655-012-1180-1 DOI：

Li-li Tao, Yan Lei, Guo-li Wang, et al. Effect of extracts from Radix Ginseng, Radix Notoginseng and Rhizoma Chuanxiong on delaying aging of vascular smooth muscle cells in aged rats[J]. Chinese Journal of Integrative Medicine, 2012,18(8):582-590. DOI： 10.1007/s11655-012-1180-1.

摘要

To observe the effect of extracts from Radix Ginseng

Radix Notoginseng and Rhizoma Chuanxiong (EXT) on delaying vascular smooth muscle cells (VSMCs) aging in aged rats. VSMCs were obtained by the modified tissue explants technique and were shown to be positive for smooth muscle α-actin (SM-α-actin) by immunohistochemistry staining. VSMCs obtained from the young rats were served as the young control group; VSMCs obtained from the old rats were treated with no drug (the old group)

with low dose extracts (20 mg/L

the EXT low-concentration group) and high dose extracts (40 mg/L

the EXT highconcentration group)

and with Probucal (10−6 mol/L

the Probucal group) as a positive control. All groups were cultured for 24 h in the medium with 10% serum for 24 h followed by another 24 h in the serum-free medium. At the end of the 48-h culture

the following analyses were performed including determination of senescence-associated β-galactosidase (SAβ-Gal) activity

flow cytometry analysis of cell cycle

real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) analyses of p16

Cyclin D1

cyclin-dependent kinase 4 (CDK4) and retinoblastoma (Rb) mRNA expression

and Western blotting analyses of p16

cyclin D1

CDK4 and phosphoretinoblastoma (pRb) protein expressions. (1) In comparison to the younger rats

VSMCs from aged rats had significantly more SAβ-Gal positive cells (P<0.01) and more cells in S phase (P<0.05). VSMCs from the all treated groups showed a significant decrease in both SAβ-Gal positive cells (P<0.05) and S phase (P<0.05) compared to the old rats. (2) Compared with the young group

VSMCs in the old group had a significant decrease in p16 and Rb mRNA expression and a significant increase in Cyclin D1 and CDK4 mRNA expression. Compared with the old group

VSMCs in the treated groups had a significant increase in p16 and Rb mRNA expression and a significant decrease in Cyclin D1 and CDK4 mRNA expression (P<0.05). (3) Compared with the young group

VSMCs in the old group had a significant decrease in p16 protein expression and a significant increase in Cyclin D1

CDK4 and pRb protein expressions (P<0.05). Compared with the old group

VSMCs in the treated groups had a significant increase in p16 protein expression and a significant decrease in cyclinD1

CDK4 and pRb protein expressions (P<0.05). VSMCs obtained from old rats showed typical signs of cellular senescence and vascular aging. EXT had an effect on delaying senescence of VSMCs in vitro by altering the p16-cyclinD/CDK-Rb pathway.

Abstract

To observe the effect of extracts from Radix Ginseng

with low dose extracts (20 mg/L

the EXT low-concentration group) and high dose extracts (40 mg/L

the EXT highconcentration group)

and with Probucal (10−6 mol/L

the Probucal group) as a positive control. All groups were cultured for 24 h in the medium with 10% serum for 24 h followed by another 24 h in the serum-free medium. At the end of the 48-h culture

the following analyses were performed including determination of senescence-associated β-galactosidase (SAβ-Gal) activity

flow cytometry analysis of cell cycle

real-time quantitative reverse transcription polymerase chain reaction (RT-PCR) analyses of p16

Cyclin D1

cyclin-dependent kinase 4 (CDK4) and retinoblastoma (Rb) mRNA expression

and Western blotting analyses of p16

cyclin D1

CDK4 and phosphoretinoblastoma (pRb) protein expressions. (1) In comparison to the younger rats

VSMCs in the old group had a significant decrease in p16 and Rb mRNA expression and a significant increase in Cyclin D1 and CDK4 mRNA expression. Compared with the old group

VSMCs in the treated groups had a significant increase in p16 and Rb mRNA expression and a significant decrease in Cyclin D1 and CDK4 mRNA expression (P<0.05). (3) Compared with the young group

VSMCs in the old group had a significant decrease in p16 protein expression and a significant increase in Cyclin D1

CDK4 and pRb protein expressions (P<0.05). Compared with the old group

VSMCs in the treated groups had a significant increase in p16 protein expression and a significant decrease in cyclinD1

关键词

vascular agingvascular smooth muscle cellsextracts from Radix GinsengRadix Notoginseng and Rhizoma Chuanxiongsenescence-associated β-galactosidasep16

Keywords

vascular agingvascular smooth muscle cellsextracts from Radix GinsengRadix Notoginseng and Rhizoma Chuanxiongsenescence-associated β-galactosidasep16

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