Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms

Zhi-ming Gao; Li Yang; Feng Huang; Ai-hua Xiong; Ning Zhou; Lan Zhou; Kong-yan Li; Jie-li Deng; Kai-yu Li; Wei Liu; Yao Chen; Wan-ting Luo; Hong Nie

doi:10.1007/s11655-012-1190-z

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Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms

OriginalPaper | Updated：2021-08-27

- Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms
- Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms
- 中国结合医学杂志（英文版） 2013年19卷第11期页码：844-852
- Affiliations：
  
  1. Department of Phamacy, Zhuhai Municipal People’s Hospital,Guangdong Province,Zhuhai,China
  2. College of Pharmacy, Jinan University,Guangzhou,China
  3. Guizhou Tongjitang Pharmaceutical Co., Ltd.,Guiyang,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30400596), the 211 Project of Jinan University, the Fundamental Research Funds for the Central Universities, Grants from Natural Science Foundation of Jinan University (No. 51204017) and Research Projects of Jinan University Challenge Cup Extracurricular Scientific Activities
- DOI：10.1007/s11655-012-1190-z
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-10-22，
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Gao, Zm., Yang, L., Huang, F. et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms., Chin. J. Integr. Med. 19, 844–852 (2013). https://doi.org/10.1007/s11655-012-1190-z

Zhi-ming Gao, Li Yang, Feng Huang, et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms[J]. Chinese Journal of Integrative Medicine, 2013,19(11):844-852.
Gao, Zm., Yang, L., Huang, F. et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms., Chin. J. Integr. Med. 19, 844–852 (2013). https://doi.org/10.1007/s11655-012-1190-z DOI：

Zhi-ming Gao, Li Yang, Feng Huang, et al. Effects of different extracts of kanggushu (抗骨疏) on osteoporosis in model rats and the underlying mechanisms[J]. Chinese Journal of Integrative Medicine, 2013,19(11):844-852. DOI： 10.1007/s11655-012-1190-z.

摘要

To investigate the preventive effects and possible underlying mechanism of different extracts of Kanggushu (抗骨疏) on osteoporosis in ovariectomized rats. One hundred and sixtyfive female SD rats were divided into 11 groups: control

sham

model

Xianling Gubao Capsule (仙灵骨葆胶囊)

nilestriol

Kanggushu aqueous extract high-

medium-

and low-dose and suet extract high-

medium-

and low-dose groups. The osteoporosis model was made by ovariectomizing the rats. The latter 8 groups were administered intragastricly with Xianling Gubao Capsule

nilestriol

Kanggushu aqueous extract and suet extract for 12 weeks

respectively

while the other 3 groups were administered orally saline. The whole body bone mineral density

bone mineral content

organ coefficient of uterus

serum estradiol and alkaline phosphatase contents

blood calcium

phosphorus

interleukin 6 and bone Gla-protein levels after treatment were monitored. Additionally

three-point bending test of femur

HE staining

and scanning electron microscope were performed to explore the pharmacodynamics and underlying mechanisms. In comparison with ovariectomized rats of model group

Kanggushu aqueous extract high-dose resulted in an increased bone mineral density

bone mineral content and organ coefficient of uterus

improved estradiol level

and improved maximum load and structural stiffness (P<0.05 or P<0.01). Two-dimensional and three-dimensional trabecular structure was also observed under HE staining and scanning electron microscopy

and the number and thickness of trabecular bone in Kanggushu aqueous extract high-dose group was significantly increased compared to the model group

while the lipid droplets in bone marrow cavity were significantly less. However

there were no significant differences in blood calcium

total serum alkaline phosphatase and bone Gla protein among different treatment groups. Overall

the osteoprotective effects of Kanggushu aqueous extract were comparable to those of nilestriol and were significantly more effective than those of Xianling Gubao Capsule. The preventive effects of Kanggushu aqueous extract might be partly due to the increased estradiol level

accelerated restoration of bone trabecular reticulate structure

and accordingly increased bone mineral density in osteoporosis rats.

Abstract

sham

model

Xianling Gubao Capsule (仙灵骨葆胶囊)

nilestriol

Kanggushu aqueous extract high-

medium-

and low-dose and suet extract high-

medium-

and low-dose groups. The osteoporosis model was made by ovariectomizing the rats. The latter 8 groups were administered intragastricly with Xianling Gubao Capsule

nilestriol

Kanggushu aqueous extract and suet extract for 12 weeks

respectively

while the other 3 groups were administered orally saline. The whole body bone mineral density

bone mineral content

organ coefficient of uterus

serum estradiol and alkaline phosphatase contents

blood calcium

phosphorus

interleukin 6 and bone Gla-protein levels after treatment were monitored. Additionally

three-point bending test of femur

HE staining

and scanning electron microscope were performed to explore the pharmacodynamics and underlying mechanisms. In comparison with ovariectomized rats of model group

Kanggushu aqueous extract high-dose resulted in an increased bone mineral density

bone mineral content and organ coefficient of uterus

improved estradiol level

and improved maximum load and structural stiffness (P<0.05 or P<0.01). Two-dimensional and three-dimensional trabecular structure was also observed under HE staining and scanning electron microscopy

and the number and thickness of trabecular bone in Kanggushu aqueous extract high-dose group was significantly increased compared to the model group

while the lipid droplets in bone marrow cavity were significantly less. However

there were no significant differences in blood calcium

total serum alkaline phosphatase and bone Gla protein among different treatment groups. Overall

accelerated restoration of bone trabecular reticulate structure

and accordingly increased bone mineral density in osteoporosis rats.

关键词

Kanggushu extractXianling Gubao CapsuleOsteoporosisestrogenmechanism

Keywords

Kanggushu extractXianling Gubao CapsuleOsteoporosisestrogenmechanism

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