Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease

Wen-juan Wang; Zhi-kui Wu; Xin-hua Zhang; Yong-mei LIU; Su-ping Fang; Chong Zhang; Wen-jun Liu; Min Li; Rong-xin Wang; Rui-gui Luo; Ping-ping Li

doi:10.1007/s11655-012-1202-z

Your Location：

Home >

Browse articles >

Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease

OriginalPaper | Updated：2021-08-27

- Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease
- Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease
- 中国结合医学杂志（英文版） 2012年18卷第9期页码：670-675
- Affiliations：
  
  1. School of Traditional Chinese Medicine, Capital Medical University,Beijing,China
  2. Laboratory of Molecular Biology, Guang’anmen Hospital, China Academy of Chinese Medical Sciences,Beijing,China
  3. Department of Haematology, 303rd Hospital of People’s Liberation Army,Nanning,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 90409003) and the National Basic Research Program (973 Program, No. 2010CB530406)
- DOI：10.1007/s11655-012-1202-z
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-8-31，
Scan for full text
Wang, Wj., Wu, Zk., Zhang, Xh. et al. Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease., Chin. J. Integr. Med. 18, 670–675 (2012). https://doi.org/10.1007/s11655-012-1202-z

Wen-juan Wang, Zhi-kui Wu, Xin-hua Zhang, et al. Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease[J]. Chinese Journal of Integrative Medicine, 2012,18(9):670-675.
Wang, Wj., Wu, Zk., Zhang, Xh. et al. Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease., Chin. J. Integr. Med. 18, 670–675 (2012). https://doi.org/10.1007/s11655-012-1202-z DOI：

Wen-juan Wang, Zhi-kui Wu, Xin-hua Zhang, et al. Effect of Yisui Shengxue Granule (益髓生血颗粒) on the oxidative damage of erythrocytes from patients with hemoglobin H disease[J]. Chinese Journal of Integrative Medicine, 2012,18(9):670-675. DOI： 10.1007/s11655-012-1202-z.

摘要

To investigate the effect of Yisui Shengxue Granule (益髓生血颗粒

YSSXG)

a complex Chinese medicine

on the oxidative damage of erythrocytes from patients with hemoglobin H (HbH) disease. Twenty-two patients with HbH disease and 22 healthy volunteers were observed. YSSXG was given to patients with HbH disease for 3 months. Before and after the 3-month treatment

blood parameters [hemoglobin (Hb)

red blood cells (RBCs)

and reticulocyte percent (Ret)] were examined; inclusion bodies in erythrocytes were observed by transmission electron microscopy (TEM); activities of antioxidant defense enzymes [superoxide dismutase (SOD)

glutathione peroxidase (GSH-Px)

and catalase (Cat)] and erythrocyte membrane malondialdehyde (MDA) concentrations were determined. In patients with HbH disease

measured values of RBC and Hb obtained from the first to the third months after treatment with YSSXG were significantly higher than before treatment (P<0.01). Measured values of Ret from the second to the third months after treatment were significantly lower than before treatment (P<0.05 and P<0.01

respectively). Prior to treatment with YSSXG

TEM images of RBCs showed the presence of numerous inclusion bodies. After treatment with YSSXG

the amount and volume of inclusion bodies decreased. Treatment with YSSXG also led to a significant increase in SOD activity (P<0.01)

a decrease in Cat activity (P<0.01)

and no significant differences in GSHPx activity (P>0.05) or MDA concentration (P>0.05). However

compared with the healthy counterparts

SOD

GSH-Px

and Cat activities presented at high levels (P<0.01) both before and after treatment. YSSXG could improve the degree of hemolysis and anemia in patients with HbH disease. The mechanism may be related to its antioxidative effects

which could elevate the activity of total SOD in erythrocytes and efficiently inhibit the oxidative precipitation of β-globin chains.

Abstract

To investigate the effect of Yisui Shengxue Granule (益髓生血颗粒

YSSXG)

a complex Chinese medicine

blood parameters [hemoglobin (Hb)

red blood cells (RBCs)

glutathione peroxidase (GSH-Px)

and catalase (Cat)] and erythrocyte membrane malondialdehyde (MDA) concentrations were determined. In patients with HbH disease

respectively). Prior to treatment with YSSXG

TEM images of RBCs showed the presence of numerous inclusion bodies. After treatment with YSSXG

the amount and volume of inclusion bodies decreased. Treatment with YSSXG also led to a significant increase in SOD activity (P<0.01)

a decrease in Cat activity (P<0.01)

and no significant differences in GSHPx activity (P>0.05) or MDA concentration (P>0.05). However

compared with the healthy counterparts

SOD

GSH-Px

which could elevate the activity of total SOD in erythrocytes and efficiently inhibit the oxidative precipitation of β-globin chains.

关键词

hemoglobin H diseaseYisui Shengxue Granuleantioxidative effectChinese Medicine

Keywords

hemoglobin H diseaseYisui Shengxue Granuleantioxidative effectChinese Medicine

references

Ooi GC, Chen FE, Chan KN, Tsang KW, Wong YH, Liang R, et al. Qualitative and quantitative magnetic resonance imaging in haemoglobin H disease: screening for iron overload. Clin Radiol 1999;54:98–102.

Chen FE, Ooi C, Ha SY, Cheung BM, Todd D, Liang R, et al. Genetic and clinical features of hemoglobin H disease in Chinese patients. N Engl J Med 2000;343:544–550.

Tongsong T, Srisupundit K, Luewan S. Outcomes of pregnancies affected by hemoglobin H disease. Int J Gynaecol Obstet 2009;104:206–208.

Wickramasinghe SN, Hughes M, Fucharoen S, Wasi P. The fate of excess β-globin chains within erythropoietic cells in α-thalassaemia-2 trait, α-thalassaemia-1 trait, haemoglobin H disease and haemoglobin Q-H disease: an electron microscopic study. Br J Haematol 1984;56:473–482.

Marengo-Rowe AJ. The thalassemias and related disorders. Proc (Bayl Univ Med Cent) 2007;20:27–31.

Schrier SL, Bunyaratvej A, Khuhapinant A, Fucharoen S, Aljurf M, Snyder LM, et al. The unusual pathobiology of hemoglobin Constant Spring red blood cells. Blood 1997;89:1762–1769.

Wagner GM, Liebhaber SA, Cutting HO, Embury SH. Hematologic improvement following splenectomy for hemoglobin-H disease. West J Med 1982;137:325–328.

Fang SP, Wu ZK, Zhang XH, Liu YM, Wang WJ, Chai LM, et al. Clinical observation on Yisui Shengxue Granule on treating 156 patients with β-thalassemia major and the molecular mechanism study. Biol Pharm Bull 2007;30:2084–2087.

Wang WJ, Wu ZK, Zhang XH, Liu YM, Fang SP, Wang RX, et al. Clinical observation of Yisui Shengxue Granule in treating 25 patients with hemoglobin H disease. J Chin Integr Med (Chin) 2008;6:153–156.

Zhang ZN, ed. Criteria for diagnosis and therapeutic effect on hematopathy. Beijing: Science Press; 2007:33.

Dodge JT, Mitchell C, Hanahan DJ. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes. Arch Biochem Biophys 1963;100:119–130.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–254.

Yuan J, Bunyaratvej A, Fucharoen S, Fung C, Shinar E, Schrier SL. The instability of the membrane skeleton in thalassemic red blood cells. Blood 1995;86:3945–3950.

Kattamis C, Touliatos N, Haidas S, Matsaniotis N. Growth of children with thalassaemia: effect of different transfusion regimens. Arch Dis Child 1970;45:502–505.

Schrier SL, Rachmilewitz E, Mohandas N. Cellular and membrane properties of alpha and beta thalassemic erythrocytes are different: implication for differences in clinical manifestations. Blood 1989;74:2194–2202.

Claster S, Chiu DT, Quintanilha A, Lubin B. Neutrophils mediate lipid peroxidation in human red cells. Blood 1984;64:1079–1084.

Scott MD, Eaton JW, Kuypers FA, Chiu DT, Lubin BH. Enhancement of erythrocyte superoxide dismutase activity: effects on cellular oxidant defense. Blood 1989;74:2542–2549.

Gunduz K, Ozturk G, Sozmen EY. Erythrocyte superoxide dismutase, catalase activities and plasmanitrite and nitrate levels in patients with Behçet disease and recurrent aphthous stomatitis. Clin Exp Dermatol 2004;29:176–179.

Çimen MY. Free radical metabolism in human erythrocytes. Clin Chim Acta 2008;390(1–2):1–11.

Beutler E, Matsumoto F, Powars D, Warner J. Increased glutathione peroxidase activity in alpha-thalassemia. Blood 1977;50:647–655.

Yenchitsomanus P, Wasi P. Increased erythrocyte superoxide dismutase activities in β-thalassaemia/haemoglobin E and in haemoglobin H diseases. J Clin Pathol 1 1983;36:329–333.

Prasartkaew S, Bunyaratvej A, Fucharoen S, Wasi P. Comparison of erythrocyte antioxidative enzyme activities between two types of hemoglobin H disease. J Clin Pathol 1986;39:1299–1303.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome

Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats

Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer