Li, Wz., Tan, Ll., Li, Qj. et al. Stabilizing the bactericidal activity of hydrogen peroxide: A brandnew function of certain Chinese herbs., Chin. J. Integr. Med. 20, 468–473 (2014). https://doi.org/10.1007/s11655-012-1241-5
Wan-zhen Li, Ling-ling Tan, Qi-jie Li, et al. Stabilizing the bactericidal activity of hydrogen peroxide: A brandnew function of certain Chinese herbs[J]. Chinese Journal of Integrative Medicine, 2014,20(6):468-473.
Li, Wz., Tan, Ll., Li, Qj. et al. Stabilizing the bactericidal activity of hydrogen peroxide: A brandnew function of certain Chinese herbs., Chin. J. Integr. Med. 20, 468–473 (2014). https://doi.org/10.1007/s11655-012-1241-5DOI:
Wan-zhen Li, Ling-ling Tan, Qi-jie Li, et al. Stabilizing the bactericidal activity of hydrogen peroxide: A brandnew function of certain Chinese herbs[J]. Chinese Journal of Integrative Medicine, 2014,20(6):468-473. DOI: 10.1007/s11655-012-1241-5.
Stabilizing the bactericidal activity of hydrogen peroxide: A brandnew function of certain Chinese herbs
摘要
To explore natural herbs to maintain the bactericidal activity of hydrogen peroxide (H). Eighteen extracts of Chinese herbs were prepared complying with the standard protocol. Each of the solutions was then mixed with 1% H2O2. The mixtures were handled with two approaches: autoclaved daily for one
two or three times; stored at room temperature from one through five years. Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) against Gram positive (Staphylococcus aureus
ATCC25923) and Gram negative (Escherichia coli
ATCC12421) bacteria. While mixed with 1% H2O2
10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving
and 8 of them preserved such level of MBC value after stored at room temperature for three years. Some Chinese herbs
i.e. R. Scutellariae
R. Coptidis
R. Bupleuri
H. Epimedii
C. Phelledendri and F. Chrysanthemi
can significantly maintain the bactericidal activity of diluted H2O2 Certain Chinese herbs can effectively stabilize the bactericidal activity of H2O2 undergoing autoclave or long-term storage. This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H2O2 usage while its stability level is promoted.
Abstract
To explore natural herbs to maintain the bactericidal activity of hydrogen peroxide (H). Eighteen extracts of Chinese herbs were prepared complying with the standard protocol. Each of the solutions was then mixed with 1% H2O2. The mixtures were handled with two approaches: autoclaved daily for one
two or three times; stored at room temperature from one through five years. Then the bactericidal activity were evaluated by assaying the minimal bactericidal concentration (MBC) and minimal inhibitory concentration (MIC) against Gram positive (Staphylococcus aureus
ATCC25923) and Gram negative (Escherichia coli
ATCC12421) bacteria. While mixed with 1% H2O2
10 out of 18 kinds of assessed Chinese herbs displayed MBC values at 1:12800 or higher after three times of autoclaving
and 8 of them preserved such level of MBC value after stored at room temperature for three years. Some Chinese herbs
i.e. R. Scutellariae
R. Coptidis
R. Bupleuri
H. Epimedii
C. Phelledendri and F. Chrysanthemi
can significantly maintain the bactericidal activity of diluted H2O2 Certain Chinese herbs can effectively stabilize the bactericidal activity of H2O2 undergoing autoclave or long-term storage. This paper reported a brandnew pharmaceutical function of Chinese herbs and provided experimental data for the potential enhancement of H2O2 usage while its stability level is promoted.
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相关机构
Henan University of Chinese Medicine
The First Affiliated Hospital of Henan University of Chinese Medicine
Department of Tradional Chinese Medicine and Integrated Tradional Chinese and Western Medicine, Peking University First Hospital
Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine
Department of Gastroenterology, Peking University First Hospital