Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B

Ze-long Jiao; Lei Li; Zi-gang Zhao; Dan Liu; Bi-wen Lin; Heng-jin Li

doi:10.1007/s11655-012-1251-3

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Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B

OriginalPaper | Updated：2021-08-27

- Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B
- Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B
- 中国结合医学杂志（英文版） 2013年19卷第10期页码：741-748
- Affiliations：
  
  1. Department of Dermatology, The Chinese People’s Liberation Army General Hospital,Beijing,China
  2. Department of Dermatology, The Navy Headquarters Clinic,Beijing,China
  3. Department of Dermatology, First Affiliated Hospital of Chinese People’s Liberation Army General Hospital,Beijing,China
- Author bio：
- Funds：
- DOI：10.1007/s11655-012-1251-3
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2012-12-3，
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Jiao, Zl., Li, L., Zhao, Zg. et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B., Chin. J. Integr. Med. 19, 741–748 (2013). https://doi.org/10.1007/s11655-012-1251-3

Ze-long Jiao, Lei Li, Zi-gang Zhao, et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B[J]. Chinese Journal of Integrative Medicine, 2013,19(10):741-748.
Jiao, Zl., Li, L., Zhao, Zg. et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B., Chin. J. Integr. Med. 19, 741–748 (2013). https://doi.org/10.1007/s11655-012-1251-3 DOI：

Ze-long Jiao, Lei Li, Zi-gang Zhao, et al. Aqueous extracts of Ocimum grasstimum inhibits lipopolysaccharide-induced interleukin-6 and interleukin-8 expression in airway epithelial cell BEAS-2B[J]. Chinese Journal of Integrative Medicine, 2013,19(10):741-748. DOI： 10.1007/s11655-012-1251-3.

摘要

To investigate the antiinflammatory activities of aqueous extract of Occimum gratissmium (OGE) with emphasis on expression of proinflammatory cytokines in Lipopolysaccharide (LPS)-stimulated epithelial cell BEAS-2B. Effects of OGE on cell viability were determined by MTT assay. mRNA expression were analyzed by and reverse transcription polymerase chain reaction (RT-PCR) and quantitative real-time PCR. Activation of kinase cascades was investigated by immunoblot. Intracellular reactive oxygen species (ROS) was analyzed by flow cytometry. OGE (<200 μg/mL) treatment or pretreatment and following LPS exposure slightly affected viability of BEAS-2B cells. Increase of interleukin (IL)-6 and IL-8 and the elevated level of intracellular ROS in LPS-stimulated BEAS-2B cells were diminished by OGE pretreatment in a dose-dependent manner. OGE suppressed inflammatory response-associated mitogen-activated protein kinases (MAPKs) and Akt activation. Additionally

OGE pretreatment increased level of cellular inhibitor of κBα (IκBα) and inhibited nuclear translocation of nuclear factor kappa B (NF-κB). These findings indicate that significant suppression of IL-6 and IL-8 expressions in LPS-stimulated BEAS-2B cells by OGE may be attributed to inhibiting activation of MAPKs and Akt and consequently suppressing nuclear translocation of NF-κB.

Abstract

关键词

Ocimum grasstimumantiinflammationlipopolysaccharidesepithelial cell

Keywords

Ocimum grasstimumantiinflammationlipopolysaccharidesepithelial cell

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