Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation

Zhen-gao Sun; Fang Lian; Qing Jia; Jin-long Sun; Ting-ting Li; Ying Guo; Jian-wei Zhang; Ning Zhang; Hui Liu; Li-hong Wang

doi:10.1007/s11655-012-1273-7

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Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation

OriginalPaper | Updated：2021-08-27

- Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation
- Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation
- 中国结合医学杂志（英文版） 2012年18卷第11期页码：846-849
- Affiliations：
  
  1. Reproductive Center, the Affiliated Hospital of Shandong, University of Traditional Chinese Medicine,Jinan,China
  2. Basic Medicine Research Institution, Shandong Academy of Medical Sciences,Jinan,China
  3. Clinical College, Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30901920), Shandong Province Natural Funded Projects (No. Q2008C17) and Shandong Province Traditional Chinese Medical Science and Technology Development Programs (No. 2009-038)
- DOI：10.1007/s11655-012-1273-7
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-10-20，
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Sun, Zg., Lian, F., Jia, Q. et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation., Chin. J. Integr. Med. 18, 846–849 (2012). https://doi.org/10.1007/s11655-012-1273-7

Zhen-gao Sun, Fang Lian, Qing Jia, et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation[J]. Chinese Journal of Integrative Medicine, 2012,18(11):846-849.
Sun, Zg., Lian, F., Jia, Q. et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation., Chin. J. Integr. Med. 18, 846–849 (2012). https://doi.org/10.1007/s11655-012-1273-7 DOI：

Zhen-gao Sun, Fang Lian, Qing Jia, et al. Effects of Er’zhi Tiangui Granule (二至天癸颗粒) on sequential expressions of integrin β 3 and its ligand osteopontin in mouse endometrium during controlled ovarian hyperstimulation[J]. Chinese Journal of Integrative Medicine, 2012,18(11):846-849. DOI： 10.1007/s11655-012-1273-7.

摘要

To investigate the effects of Er’zhi Tiangui Granule (二至天癸颗粒

ETG) on sequential expressions of integrinβ3 and its ligand osteopontin in the mouse endometrium during controlled ovarian hyperstimulation (COH) and implantation period. Seventy-five Mature female Kunming mice were randomly divided into 3 groups

a normal control group

a model group

and a treatment group administrated with ETG for 10 days

25 in each group. After mated with male mice

every 5 mice were sacrified in each group at the 0

2nd

4th

6th

and 8th days to take their endometrium. In-situ hybridization was used to detect the expressions of integrinβ3 and osteopontin in the endometrium. mRNA expressions of integrinβ3 and osteopontin in the endometrium during implantation period showed similar time sequence rules in the treatment group to those in the normal control group; the peak values of them were a little lower in the treatment group than the normal control without significant differences. In the model group

integrinβ3 mRNA expression was higher at the 2nd day

obviously lower at the 4th and 6th days

and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th

6th

and 8th days

compared with the normal control and the treatment groups (P<0.05

P<0.01). COH might influence the sequential expressions of integrinβ3 and its ligand osteopontin

bring forward the integrinβ3 expression peak

impact on the cooperation of integrinβ3 and osteopontin

so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrinβ3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.

Abstract

To investigate the effects of Er’zhi Tiangui Granule (二至天癸颗粒

a normal control group

a model group

and a treatment group administrated with ETG for 10 days

25 in each group. After mated with male mice

every 5 mice were sacrified in each group at the 0

2nd

4th

6th

integrinβ3 mRNA expression was higher at the 2nd day

obviously lower at the 4th and 6th days

and insignificantly lower at the 8th day; and osteopontin expression was remarkably lower at the 4th

6th

and 8th days

compared with the normal control and the treatment groups (P<0.05

P<0.01). COH might influence the sequential expressions of integrinβ3 and its ligand osteopontin

bring forward the integrinβ3 expression peak

impact on the cooperation of integrinβ3 and osteopontin

so as to damage the endometrial receptivity. ETG could regulate the sequential expressions of integrinβ3 and its ligand osteopontin to improve the mouse endometrial receptivity during COH.

关键词

Kidney-tonifyingEr’zhi Tiangui Granulecontrolled ovarian hyperstimulationendometrial receptivityintegrinβ3osteopontinmouse

Keywords

Kidney-tonifyingEr’zhi Tiangui Granulecontrolled ovarian hyperstimulationendometrial receptivityintegrinβ3osteopontinmouse

references

Lian F, Sun ZG, Mu L, Zhang JW, Liu YH, Zhang N, et al. Experimental study on effect of Er’zhi Tiangui Granule in improving quality of oocyte and its correlation with level of insulinlike growth factor-1R mRNA expression in ovary of mice. Chin J Integr Tradit West Med (Chin) 2006;26:431–434.

Miao MS. Experimental animal and animal experiment techniques. 1st ed. Beijing: China Press of Traditional Chinese Medcine; 2003:143–144.

Rout UK, Wang J, Paria BC, Armant DR. Alpha5beta1, alpha5beta3 and the platelet-associated integrin alpha2b beta3 coordinately regulate adhesion and migration of differentiating mouse trophoblast cells. Dev Biol 2004;268:135–151.

Murray MJ, Lessey BA. Embryo implantation and tumor metastasis: common pathways of invasion and angiogenesis. Semi Reprod Endocrinol 1999;17:275–290.

Johnson GA, Burghardt RC, Joyce MM, Spencer TE, Bazer FW, Pfarrer C, et al. Osteopontin expression in uterine stroma indicates a decidualization-like differentiation during ovine pregnancy. Biol Reprod 2003;68:1951–1958.

Johnson GA, Burghardt RC, Bazer FW. Osteopontin: roles in implantation and placentation. Biol Reprod 2003;69:1458–1471.

Lessey BA, Castelbaum AJ. Integrins and implantation in the human. Rev Endocr Metab Disord 2002;3:107–117.

Sun ZZ, Ma YK, Wang ZR, Guan YM. Expressions of integrinβ3 and epidermal growth factor receptor in the endometrium during implantation window phase after different controlled ovarian hyperstimulation therapy and their importance. Chin J Pract Gynecol Obstetr (Chin) 2008;24:383–384.

Ledee-Bataille N, Lapree-Delage G, Taupin JL, Dubanchet S, Frydman R, Chaouat G. Concentration of leukaemia inhibitory factor (LIF) in uterine flushing fluid is highly predictive of embryo implantation. Hum Reprod 2002;17:213–218.

Zhao LY, Xia T, Song WJ. Progress of Chinese and Western medicine treatment on endometrial receptivity during IVF. Chin J Integr Tradit West Med (Chin) 2011;31:1581–1584.

Wang YN, Song DR. Progress of study on Chinese medicine in improving endometrial receptivity. Chin J Integr Tradit West Med (Chin) 2009;29:666–668.

Wang MM, Hao CF, Bao HC, Zhang N, Ge L. Clinical efficacy of Chinese herbal retention enema combined with intrauterine douching for patients with endometritis. Chin J Integr Tradit West Med (Chin) 2011;31:639–642.

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