Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway

Ran Wang; Wei-hong Cong; Gang Guo; Xiang-xin Li; Xue-liang Chen; Xiao-ning Yu; Hao Li

doi:10.1007/s11655-012-1297-z

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Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway

OriginalPaper | Updated：2021-08-27

- Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway
- Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway
- 中国结合医学杂志（英文版） 2012年18卷第12期页码：934-941
- Affiliations：
  
  1. Department of Hematology, Qilu Hospital of Shandong University,Jinan,China
  2. Research Center, Xiyuan Hospital of China Academy of Chinese Medical Sciences,Beijing,China
  3. Department of Scientific Research, Qilu Hospital of Shandong University,Jinan,China
  4. Department of Health Care, Qilu Hospital of Shandong University,Jinan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81102710)
- DOI：10.1007/s11655-012-1297-z
  中图分类号：
- 纸质出版日期：2012，
  
  网络出版日期：2012-12-13，
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Wang, R., Cong, Wh., Guo, G. et al. Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway., Chin. J. Integr. Med. 18, 934–941 (2012). https://doi.org/10.1007/s11655-012-1297-z

Ran Wang, Wei-hong Cong, Gang Guo, et al. Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway[J]. Chinese Journal of Integrative Medicine, 2012,18(12):934-941.
Wang, R., Cong, Wh., Guo, G. et al. Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway., Chin. J. Integr. Med. 18, 934–941 (2012). https://doi.org/10.1007/s11655-012-1297-z DOI：

Ran Wang, Wei-hong Cong, Gang Guo, et al. Synergism between carnosic acid and arsenic trioxide on induction of acute myeloid leukemia cell apoptosis is associated with modulation of PTEN/Akt signaling pathway[J]. Chinese Journal of Integrative Medicine, 2012,18(12):934-941. DOI： 10.1007/s11655-012-1297-z.

摘要

To investigate the synergistic effects of carnosic acid (CA) with arsenic trioxide (As2O3) on proliferation and apoptosis in HL-60 human myeloid leukemia cells

and the major cellular signaling pathway involved in these effects. HL-60 cellular proliferation was measured by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) analysis. Cell cycle distribution and apoptosis were monitored by flow cytometry. The activation of casepase-9

Bcl-2-associated agonist of cell death (BAD)

p-BAD

p27

phosphatase and tensin homolog deleted on chromosome ten (PTEN)

Akt

p-Akt was assessed by Western blot analysis. The expression of PTEN mRNA was tested by reverse transcription polymerase chain reaction (RT-PCR) analysis. CA reduced HL-60 cell viability in a dose- and time-dependent manner

and induced G1 arrest and apoptosis. Moreover

CA upregulated PTEN expression

blocked the Akt signaling pathway

subsequently inhibited phosphorylation of BAD

reactivated caspase-9

and elevated levels of p27. CA also augmented these effects of As2O3. CA might be a novel candidate of the combination therapy for leukemia treatment; these effects were apparently associated with the modulation of PTEN/Akt signaling pathway.

Abstract

To investigate the synergistic effects of carnosic acid (CA) with arsenic trioxide (As2O3) on proliferation and apoptosis in HL-60 human myeloid leukemia cells

and the major cellular signaling pathway involved in these effects. HL-60 cellular proliferation was measured by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) analysis. Cell cycle distribution and apoptosis were monitored by flow cytometry. The activation of casepase-9

Bcl-2-associated agonist of cell death (BAD)

p-BAD

p27

phosphatase and tensin homolog deleted on chromosome ten (PTEN)

Akt

and induced G1 arrest and apoptosis. Moreover

CA upregulated PTEN expression

blocked the Akt signaling pathway

subsequently inhibited phosphorylation of BAD

reactivated caspase-9

关键词

carnosic acidcombination therapyacute myeloid leukemiaapoptosisPTEN/Akt signaling pathway

Keywords

carnosic acidcombination therapyacute myeloid leukemiaapoptosisPTEN/Akt signaling pathway

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