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Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke
Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke
- 中国结合医学杂志（英文版） 2014年20卷第1期 页码：11-18
- Affiliations：
Department of Neurology, Dongzhimen Hospital Affiliated to Beijing University of Chinese Medicine,Beijing,China
- Author bio：
- Funds：Supported by the National Major Foundation Research Development Plan (No. 2003CB517102);State Major Science and Technology Special Projects “Major Creation of New Drugs” (No. 2009ZX09502-028).
DOI：10.1007/s11655-013-1194-8
中图分类号：纸质出版日期：2014，
网络出版日期：2013-3-25，
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Liu, L., Gao, Y. & Ma, B. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke., Chin. J. Integr. Med. 20, 11–18 (2014). https://doi.org/10.1007/s11655-013-1194-8
Lu Liu, Ying Gao, Bin Ma. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke[J]. Chinese Journal of Integrative Medicine, 2014,20(1):11-18.
Liu, L., Gao, Y. & Ma, B. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke., Chin. J. Integr. Med. 20, 11–18 (2014). https://doi.org/10.1007/s11655-013-1194-8 DOI：
Lu Liu, Ying Gao, Bin Ma. Exploring molecular mechanism underlying Chinese medicine syndrome: A study on correlation between Chinese medicine syndrome and biomarkers for ischemic stroke[J]. Chinese Journal of Integrative Medicine, 2014,20(1):11-18. DOI： 10.1007/s11655-013-1194-8.
摘要
To investigate whether Chinese medicine (CM) syndrome is associated with particular molecular mechanism
we explored the correlation between CM syndrome and changes of intercellular adhesion molecule-1 (ICAM-1)
matrix metalloproteinase 9 (MMP-9) and heat shock protein 70 (HSP70) in patients with ischemic stroke
which were reported to play an important role in the inflammatory and apoptosis cascade. CM syndrome factors of 175 patients with ischemic stroke were assessed using Ischemic Stroke CM Syndrome Factor Diagnostic Scale (ISTSFDS). The patients were grouped according to the main syndrome factor combinations at different time points based on distribution probability of syndrome factor combinations. Blood levels of ICAM-1
MMP-9 and HSP70 were quantified by enzyme-linked immunosorbent assay. ICAM-1 expression was significantly higher in the internal-wind+phlegm-dampness+blood-stasis
phlegmdampness+ blood-stasis
internal-fire+phlegm-dampness+blood-stasis group than that in the blood-stasis+qideficiency group within 72 h from stroke onset (P <0.05); HSP70 expression was significantly lower in the phlegm-dampness+blood-stasis
internal-fire+phlegm-dampness+blood-stasis
blood-stasis group than that in the phlegm-dampness+blood-stasis+qi-deficiency group on the 7th day from stroke onset (P<0.05). Phlegm-dampness and blood-stasis exist through the whole process of ischemic stroke. An increased level of ICAM-1 and a reduced level of HSP70 reflect the pathological state of phlegm-stasis mutual binding. These results suggest that inflammation and apoptosis induced by cerebral vascular injury in the pathological processes of ischemic stroke are more prominent in the excess syndrome state like phlegm-dampness and blood-stasis.
Abstract
To investigate whether Chinese medicine (CM) syndrome is associated with particular molecular mechanism
we explored the correlation between CM syndrome and changes of intercellular adhesion molecule-1 (ICAM-1)
matrix metalloproteinase 9 (MMP-9) and heat shock protein 70 (HSP70) in patients with ischemic stroke
which were reported to play an important role in the inflammatory and apoptosis cascade. CM syndrome factors of 175 patients with ischemic stroke were assessed using Ischemic Stroke CM Syndrome Factor Diagnostic Scale (ISTSFDS). The patients were grouped according to the main syndrome factor combinations at different time points based on distribution probability of syndrome factor combinations. Blood levels of ICAM-1
MMP-9 and HSP70 were quantified by enzyme-linked immunosorbent assay. ICAM-1 expression was significantly higher in the internal-wind+phlegm-dampness+blood-stasis
phlegmdampness+ blood-stasis
internal-fire+phlegm-dampness+blood-stasis group than that in the blood-stasis+qideficiency group within 72 h from stroke onset (P <0.05); HSP70 expression was significantly lower in the phlegm-dampness+blood-stasis
internal-fire+phlegm-dampness+blood-stasis
blood-stasis group than that in the phlegm-dampness+blood-stasis+qi-deficiency group on the 7th day from stroke onset (P<0.05). Phlegm-dampness and blood-stasis exist through the whole process of ischemic stroke. An increased level of ICAM-1 and a reduced level of HSP70 reflect the pathological state of phlegm-stasis mutual binding. These results suggest that inflammation and apoptosis induced by cerebral vascular injury in the pathological processes of ischemic stroke are more prominent in the excess syndrome state like phlegm-dampness and blood-stasis.
关键词
ischemic strokeChinese medicine syndromeintercellular adhesion molecule-1matrix metalloproteinase 9heat shock protein 70
Keywords
ischemic strokeChinese medicine syndromeintercellular adhesion molecule-1matrix metalloproteinase 9heat shock protein 70
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