Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion

Xin-liang Du; Feng Sui; Hai-ru Huo; Hong-wei Zhang; Kan Luo; Lan-fang Li; Shu-ying Guo; Ting-liang Jiang

doi:10.1007/s11655-013-1325-2

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Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion

OriginalPaper | Updated：2021-08-27

- Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion
- Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion
- 中国结合医学杂志（英文版） 2013年19卷第11期页码：826-835
- Affiliations：
  
  1. Tang Center for Herbal Medicine Research, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
  2. Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 90209006;81274112) and Beijing Municipal Natural Science Foundation (No. 7112098).
- DOI：10.1007/s11655-013-1325-2
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2013-6-10，
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Du, Xl., Sui, F., Huo, Hr. et al. Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion., Chin. J. Integr. Med. 19, 826–835 (2013). https://doi.org/10.1007/s11655-013-1325-2

Xin-liang Du, Feng Sui, Hai-ru Huo, et al. Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion[J]. Chinese Journal of Integrative Medicine, 2013,19(11):826-835.
Du, Xl., Sui, F., Huo, Hr. et al. Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion., Chin. J. Integr. Med. 19, 826–835 (2013). https://doi.org/10.1007/s11655-013-1325-2 DOI：

Xin-liang Du, Feng Sui, Hai-ru Huo, et al. Reciprocal effects of Guizhi decoction (桂枝汤) to the Guizhi decoction syndrome by toll-like receptor mRNA expression and cytokines secretion[J]. Chinese Journal of Integrative Medicine, 2013,19(11):826-835. DOI： 10.1007/s11655-013-1325-2.

摘要

To explore the pathological mechanisms of Guizhi Decoction (桂枝汤) syndrome and the therapeutic molecular mechanisms of the Guizhi Decoction

Mahuang Decoction (麻黄汤)

Sangju Decoction (桑菊饮) and Yinqiao Powder (银翘散)

as well as the potentially biological basis that Guizhi Decoction is most effective only for the patients with Guizhi Decoction syndrome in clinical practice. We first got serum samples from the patients suffering from both upper respiratory tract infection and Guizhi Decoction syndrome identified by the doctors of Chinese medicine (CM) in the clinic. Four formulas with therapeutic actions of pungent warmth or pungent coolness for superficial syndromes were chosen and four kinds of rat serum samples each containing one of the above-mentioned herbal formulas were collected

then the effects of Guizhi Decoction syndromes’ patient serum as well as the effects of sera containing the formulas after being stimulated by the patient serum samples on both the mRNA expression of certain toll-like receptor (TLR) subtypes and the release of some inflammatory cytokines in RAW264.7 cells were tested and analyzed in vitro. The expression of TLR-3

TLR-4 and TLR-9 mRNA among the 9 tested TLR subforms were up-regulated in the macrophages stimulated by the sera from untreated upper respiratory infection patients with the Guizhi Decoction syndrome (symptomcomplex). The products such as interleukin (IL)-1β

IL-6

tumor necrosis factor (TNF)-α and interferon (IFN)-β from stimulated macrophages through TLR signaling pathways were also increased correspondingly. Interestingly

the changes induced by the Guizhi Decoction syndrome patients’ sera were masked significantly after the macrophages were incubated with the sera from donors treated with Guizhi Decoction. Similarly

the three other exterior-releasing formulas were all effective in reversing the up-regulated changes of certain TLR subforms to different degrees

but both the number of targeted TLRs and efficacy of them seemed to be inferior to that of Guizhi Decoction. Evidence from these experiments might contribute to the scientific explanation of both the pharmacological mechanisms of Guizhi Decoction and also the CM theory that Guizhi Decoction is specifically prescribed for the treatment of Guizhi Decoction syndrome (The gearing formula to the symptom-complex).

Abstract

To explore the pathological mechanisms of Guizhi Decoction (桂枝汤) syndrome and the therapeutic molecular mechanisms of the Guizhi Decoction

Mahuang Decoction (麻黄汤)

Sangju Decoction (桑菊饮) and Yinqiao Powder (银翘散)

IL-6

tumor necrosis factor (TNF)-α and interferon (IFN)-β from stimulated macrophages through TLR signaling pathways were also increased correspondingly. Interestingly

the changes induced by the Guizhi Decoction syndrome patients’ sera were masked significantly after the macrophages were incubated with the sera from donors treated with Guizhi Decoction. Similarly

the three other exterior-releasing formulas were all effective in reversing the up-regulated changes of certain TLR subforms to different degrees

关键词

Chinese MedicineGuizhi DecoctionToll-like receptorformulasymptom-complex

Keywords

Chinese MedicineGuizhi DecoctionToll-like receptorformulasymptom-complex

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