Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats

Yue Shi; Xiao-chun Liang; Qun-li Wu; Lian-qing Sun; Ling Qu; Li Zhao; Pu-yan Wang

doi:10.1007/s11655-013-1352-7

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Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats

OriginalPaper | Updated：2021-08-27

- Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats
- Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats
- 中国结合医学杂志（英文版） 2013年19卷第2期页码：104-111
- Affiliations：
  
  Department of Traditional Chinese Medicine, Translational Medicine Center, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30572438)
- DOI：10.1007/s11655-013-1352-7
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2013-1-31，
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Shi, Y., Liang, Xc., Wu, Ql. et al. Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats., Chin. J. Integr. Med. 19, 104–111 (2013). https://doi.org/10.1007/s11655-013-1352-7

Yue Shi, Xiao-chun Liang, Qun-li Wu, et al. Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats[J]. Chinese Journal of Integrative Medicine, 2013,19(2):104-111.
Shi, Y., Liang, Xc., Wu, Ql. et al. Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats., Chin. J. Integr. Med. 19, 104–111 (2013). https://doi.org/10.1007/s11655-013-1352-7 DOI：

Yue Shi, Xiao-chun Liang, Qun-li Wu, et al. Effects of Jinmaitong Capsule (筋脉通胶囊) on ciliary neurotrophic factor in sciatic nerves of diabetes mellitus rats[J]. Chinese Journal of Integrative Medicine, 2013,19(2):104-111. DOI： 10.1007/s11655-013-1352-7.

摘要

To study the effects of the Chinese medicine Jinmaitong Capsule (筋脉通胶囊

JMT) on the pathomorphology of sciatic nerves

ciliary neurotrophic factor (CNTF)

and the mRNA expressions of CNTF in rats with streptozotocin-induced diabetes mellitus (STZ-DM). The animal model was established by one time intraperitoneal injection of streptozotocin. The rats were simply divided by random into 5 groups including model group

low-dose JMT group (JL)

medium-dose JMT group (JM)

high-dose JMT group (JH) and neurotropin group. For each of the above 5 groups

a group of 10 normal Wistar rats matched in body weight

age and gender were set as normal group. Intragastric administrations were started after the animal model established. The JL group were administered with five times the JMT dose recommended for a human adult; the JM group were administered with ten times the JMT dose recommended for a human adult; the JH group were administered with twenty times the JMT dose recommended for a human adult. The neurotropin group was administered with ten times the neurotropin dose recommended for a human adult. All rats were given intragastric administration for 16 weeks and then killed. In the 4th

8th

12th

16th week

body weight and blood glucose level were detected before and after the intervention. The morphologic changes of the sciatic nerves were observed by optical microscope and transmission electron microscope. The CNTFmRNA expressions were detected by real-time fluorescent quantitative polymerase chain protein

and the CNTF protein expressions were detected by immunohistochemical method. The blood glucose levels of the STZ-DM rats were much higher than normal group (P<0.01)

and there was no apparent difference between any treatment groups and the model group (P>0.05). Before and after the intervention in the 4th

8th

12th

16th week

there were no significant differences in the body weight among all the groups (P>0.05). The sciatic nerves of STZ-DM rats might have pathomorphological changes in axons

myelin sheaths

and interstitium. The levels of CNTF and CNTF-mRNA expressions in the STZ-DM rats were both significantly decreased (P<0.01). The sciatic nerves of STZ-DM rats might have pathomorphological changes in axons

myelin sheaths

and interstitium. JMT could improve the pathomorphology of sciatic nerves by increasing CNTF’s and CNTF-mRNA expressions in sciatic nerve tissues

and promote the repair and regeneration of damaged nerve fibers.

Abstract

To study the effects of the Chinese medicine Jinmaitong Capsule (筋脉通胶囊

JMT) on the pathomorphology of sciatic nerves

ciliary neurotrophic factor (CNTF)

low-dose JMT group (JL)

medium-dose JMT group (JM)

high-dose JMT group (JH) and neurotropin group. For each of the above 5 groups

a group of 10 normal Wistar rats matched in body weight

8th

12th

16th week

and the CNTF protein expressions were detected by immunohistochemical method. The blood glucose levels of the STZ-DM rats were much higher than normal group (P<0.01)

and there was no apparent difference between any treatment groups and the model group (P>0.05). Before and after the intervention in the 4th

8th

12th

16th week

there were no significant differences in the body weight among all the groups (P>0.05). The sciatic nerves of STZ-DM rats might have pathomorphological changes in axons

myelin sheaths

and interstitium. JMT could improve the pathomorphology of sciatic nerves by increasing CNTF’s and CNTF-mRNA expressions in sciatic nerve tissues

and promote the repair and regeneration of damaged nerve fibers.

关键词

diabetic peripheral neuropathyciliary neurotrophic factormorphologyJinmaitong Capsule

Keywords

diabetic peripheral neuropathyciliary neurotrophic factormorphologyJinmaitong Capsule

references

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Lan Y, Ke MY, Zhang SH, Li F, Liang XC. Gastric emptying of rats at different stages of diabetes mellitus and its response to Chinese traditional medicines with effects of promoting blood circulation and dissolving blood stasis. Acta Acad Med Sin 2000;22:411–415.

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