Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury
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OriginalPaper|Updated:2021-08-27
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Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury
Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury
中国结合医学杂志(英文版)2013年19卷第2期 页码:132-136
Affiliations:
1. Jinan University,Guangzhou,China
2. Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
Author bio:
Funds:
Supported by Project of Natural Science Foundation of Guangdong (No. 9151063201000028) and Project of Science and Technology of Guangdong (No. 2011B031800284)
Zhang, Ym., Zhang, Yq., Cheng, Sb. et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury., Chin. J. Integr. Med. 19, 132–136 (2013). https://doi.org/10.1007/s11655-013-1353-6
Yi-min Zhang, Yu-qing Zhang, Shao-bing Cheng, et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury[J]. Chinese Journal of Integrative Medicine, 2013,19(2):132-136.
Zhang, Ym., Zhang, Yq., Cheng, Sb. et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury., Chin. J. Integr. Med. 19, 132–136 (2013). https://doi.org/10.1007/s11655-013-1353-6DOI:
Yi-min Zhang, Yu-qing Zhang, Shao-bing Cheng, et al. Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury[J]. Chinese Journal of Integrative Medicine, 2013,19(2):132-136. DOI: 10.1007/s11655-013-1353-6.
Effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injury
摘要
To observe the effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injuny. Thirty SD rats were randomly and equally allocated to the sham-operated
the model and the acupuncture groups. The traumatic brain injury model was established by the free drop method. For the rats in the acupuncture group
acupuncture was applied once a day for 7 days. Brain histotomy was carried out when treatments were completed. Immunohistochemical techniques were adopted to detect the cells that express nestin
neurofilament proteins (NF)-200 and glial fibrillary acidic proteins (GFAP)
the markers of neural stem cells
neurons
astrocytes respectively. Compared to the sham-operated group
the number of nestin-positive cells and NF-200-positive cells in brain tissues was decreased significantly in the model group (P < 0.01)
whereas the number of GFAP-positive cells was significantly increased P<0.01). Compared to the model group
the positive cells of nestin
NF-200
GFAP in brain tissues in the acupuncture group were increased obviously (P<0.01). Acupuncture can significantly increase the number of nestin-positive cells
NF-200-positive cells and GFAP-positive cells
indicating the significant increase of neural stem cells
neurons and astrocytes in number. Acupuncture can improve neuranagenesis by promoting the proliferation and differentiation of neural stem cells in brain tissues. This might be one of the mechanisms for acupuncture to treat traumatic brain injury and to promote the repair of nervous function.
Abstract
To observe the effect of acupuncture on proliferation and differentiation of neural stem cells in brain tissues of rats with traumatic brain injuny. Thirty SD rats were randomly and equally allocated to the sham-operated
the model and the acupuncture groups. The traumatic brain injury model was established by the free drop method. For the rats in the acupuncture group
acupuncture was applied once a day for 7 days. Brain histotomy was carried out when treatments were completed. Immunohistochemical techniques were adopted to detect the cells that express nestin
neurofilament proteins (NF)-200 and glial fibrillary acidic proteins (GFAP)
the markers of neural stem cells
neurons
astrocytes respectively. Compared to the sham-operated group
the number of nestin-positive cells and NF-200-positive cells in brain tissues was decreased significantly in the model group (P < 0.01)
whereas the number of GFAP-positive cells was significantly increased P<0.01). Compared to the model group
the positive cells of nestin
NF-200
GFAP in brain tissues in the acupuncture group were increased obviously (P<0.01). Acupuncture can significantly increase the number of nestin-positive cells
NF-200-positive cells and GFAP-positive cells
indicating the significant increase of neural stem cells
neurons and astrocytes in number. Acupuncture can improve neuranagenesis by promoting the proliferation and differentiation of neural stem cells in brain tissues. This might be one of the mechanisms for acupuncture to treat traumatic brain injury and to promote the repair of nervous function.
关键词
traumatic brain injuryacupunctureneural stem cellsproliferation and differentiationrats
Keywords
traumatic brain injuryacupunctureneural stem cellsproliferation and differentiationrats
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Acupuncture and Moxibustion Department, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University
The First Hospital Affiliated to Tianjin University of Traditional Chinese Medicine
The Second Hospital Affiliated to Tianjin University of Traditional Chinese Medicine
Affiliated Hospital of Tianjin Academy of Traditional Chinese Medicine
Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences