Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer

Hua Sui; Hui-rong Zhu; Jie Wu; Alexander Yu Nikitin; Jian-feng Cai; Zhong-ze Fan; Qi Li

doi:10.1007/s11655-013-1452-4

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Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer

OriginalPaper | Updated：2021-08-27

- Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer
- Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer
- 中国结合医学杂志（英文版） 2014年20卷第8期页码：610-617
- Affiliations：
  
  1. Department of Medical Oncology, Shuguang Hospital & Putuo Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute,Tampa,USA
  3. Department of Biomedical Sciences, Cornell University, Ithaca,USA
  4. Department of Chemistry, University of South Florida,Tampa,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No.81202812), Science and Technology Commission of Shanghai Municipality (No. 10ZR1427400), Program of Shanghai Municipal Education Commission (No. 09YZ132, 2011JW57), and Shanghai Municipal Health Bureau (No. 2011ZJ030, 20114Y013)
- DOI：10.1007/s11655-013-1452-4
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-4-1，
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Sui, H., Zhu, Hr., Wu, J. et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer., Chin. J. Integr. Med. 20, 610–617 (2014). https://doi.org/10.1007/s11655-013-1452-4

Hua Sui, Hui-rong Zhu, Jie Wu, et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer[J]. Chinese Journal of Integrative Medicine, 2014,20(8):610-617.
Sui, H., Zhu, Hr., Wu, J. et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer., Chin. J. Integr. Med. 20, 610–617 (2014). https://doi.org/10.1007/s11655-013-1452-4 DOI：

Hua Sui, Hui-rong Zhu, Jie Wu, et al. Effects of Jianpi Jiedu Recipe (健脾解毒方) on reversion of P-glycoprotein-mediated multidrug resistance through COX-2 pathway in colorectal cancer[J]. Chinese Journal of Integrative Medicine, 2014,20(8):610-617. DOI： 10.1007/s11655-013-1452-4.

摘要

To evaluate the underlying mechanism of Jianpi Jiedu Recipe (健脾解毒方

JJR) in the reversion of multidrug resistance concerning colorectal cancer in vitro and in vivo. Mice were treated orally with JJR at a daily 4.25 g/(kg·day) or injected with vinblastine (VCR) 2.5 mg/(kg·day) for 3 weeks after having been inoculated with HCT8/V cells; tumor tissues were assayed by hematoxylin and eosin staining. Firstly

the effects of JJR on the expression of cyclooxygenase-2 (COX-2) were tested by real-time polymerase chain reaction (PCR) technique and COX-2 gene silenced by siRNA. Secondly

the variation of intracellular concentration of oxaliplatin (L-OHP) was evaluated by the inductively coupled plasma mass spectroscopy (ICPMS) in HCT8/V and its COX-2 siRNA cells; the concentration of JJR combined with chemotherapeutic drugs and the reverse effect of multidrug resistance (MDR) in HCT8/V cells was evaluated by the MTT assay. Thirdly

real-time quantitative PCR and Western blot analysis were used to detect the multidrug resistance gene 1 (MDR1) mRNA and P-gp expression. JJR had an inhibitory effect on the growth of tumors in vivo

and it

in combination with chemotherapeutic drugs

could reverse the drug-resistance of HCT8/V cells and increase the sensitivity of HCT8/V cells to VCR

DDP

5-Fu

and THP. ICP-MS results showed that JJR could increase the concentration of drugs in HCT8/V cells (P<0.01). Furthermore

it was shown that JJR could reverse drug resistance of colorectal cancer cells by decreasing MDR1 expression and P-gp level via downregulation of COX-2

which has been represented as one of the major mechanisms that contributes to the MDR phenotype (P<0.01). JJR reversed multidrug resistance and enhanced the sensitivity to chemotherapy

which could be attributed to the down-regulation of COX-2 in MDR1/P-gp-mediated MDR colorectal cancer after chemotherapy.

Abstract

To evaluate the underlying mechanism of Jianpi Jiedu Recipe (健脾解毒方

the effects of JJR on the expression of cyclooxygenase-2 (COX-2) were tested by real-time polymerase chain reaction (PCR) technique and COX-2 gene silenced by siRNA. Secondly

real-time quantitative PCR and Western blot analysis were used to detect the multidrug resistance gene 1 (MDR1) mRNA and P-gp expression. JJR had an inhibitory effect on the growth of tumors in vivo

and it

in combination with chemotherapeutic drugs

could reverse the drug-resistance of HCT8/V cells and increase the sensitivity of HCT8/V cells to VCR

DDP

5-Fu

and THP. ICP-MS results showed that JJR could increase the concentration of drugs in HCT8/V cells (P<0.01). Furthermore

it was shown that JJR could reverse drug resistance of colorectal cancer cells by decreasing MDR1 expression and P-gp level via downregulation of COX-2

which has been represented as one of the major mechanisms that contributes to the MDR phenotype (P<0.01). JJR reversed multidrug resistance and enhanced the sensitivity to chemotherapy

which could be attributed to the down-regulation of COX-2 in MDR1/P-gp-mediated MDR colorectal cancer after chemotherapy.

关键词

colorectal cancermultidrug resistanceP-glycoproteinmultidrug resistance genecyclooxygenase-2Jianpi Jiedu RecipeChinese Medicine

Keywords

colorectal cancermultidrug resistanceP-glycoproteinmultidrug resistance genecyclooxygenase-2Jianpi Jiedu RecipeChinese Medicine

references

Ahmed FE. Molecular markers that predict response to colon cancer therapy. Expert Rev Mol Diagn 2005;5:353–375.

Sutoh I, Kohno H, Nakashima Y, Hishikawa Y, Tabara H, Tachibana M, et al. Concurent expressions of matallothionein, glutathione S-tansferase-, and P-glycoprotein in colorectal cancers. Dis Colon Rectum 2000, 43:221–232.

Lazaris AC, Kavantzas NG, Zorzos HS, Tsavaris NV, Davaris PS. Markers of drug resistance in relapsing colon cancer. J Cancer Res Clin Oncol 2002;128:114–118.

Kang CD, Ahn BK, Jeong CS, Kim KW, Lee HJ, Yoo SD, et al. Downregulation of JNK/SAPK activity is associated with the cross-resistance to P-glycoprotein-unrelated drugs in multidrug-resistant FM3A/M cells overexpressing P-glycoprotein experimental. Cell Res 2000;256:300–307.

Smit JW, Huisman MT, van Tellingen O, Wiltshire HR, Schinkel AH. Absence of pharmacological blocking of placental P-glycoprotein profoundly increases fetal drug exposure. J Clin Invest 1999;104:1441–1447.

Kalle AM, Rizvi A. Inhibition of bacterial multidrug resistance by celecoxib, a cyclooxygenase-2 inhibitor. Antimicrob Agents Chemother 2011;55:439–442.

Chang CY, Huang ZN, Yu HH, Chang LH, Li SL, Chen YP. The adjuvant effects of Antrodia Camphorata extracts combined with anti-tumor agents on multidrug resistant human hepatoma cells. J Ethnopharmacol 2008;118:387–395.

Li Q, Sui H, Liu X, Qin JM, Yin Ph, Fan ZZ. Reversal effect of Jianpi Jiedu Recioe on JNK/SAPK signal transduction pathway-mediated multidrug resistance in human colon carcinoma cells. Chin J Tradit Med Pharm (Chin) 2012;27:731–735.

Sui H, Jin BH, Yin PH, Fan ZZ, LI Q. Reversal effect of Jianpi Jiedu Recioe on multidrug resistance in human colon carcinoma cells. Chin J Exp Tradit Med Formul (Chin) 2012;18:181–185.

Xiu, SY. The experimental method of pharmacology. Beijing: The People’s Public Health Publisher; 1986:985–924.

Wu Z, Min L, Chen D, Hao D, Duan Y, Qiu G, et al. Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma. PLoS One 2011;6:e14648.

Sui H, Zhou SF, Wang Y, Liu X, Zhou LH, Yin PH, et al. COX-2 contributes to P-glycoprotein-mediated multidrug resistance via phosphorylation of c-Jun at Ser63/73 in colorectal cancer. Carcinogenesis 2011;32:667–675.

Morrison JG, White P, McDougall S, Firth JW, Woolfrey SG, Graham MA, et al. Validation of a highly sensitive ICPMS method for the determination of platinum in biofluids: application to clinical pharmacokinetic studies with oxaliplatin. J Pharm Biomed Anal 2000;24:1–10.

Sui H, Fan ZZ, Li Q. Signal transduction pathways and transcriptional mechanisms of ABCB1/Pgp-mediated multiple drug resistance in human cancer cells. J Int Med Res, 2012;40:426–435.

Sui H, Zhou LH, Yin PH, Wang Y, Fan ZZ, Zhou SF, et al. JNK signal transduction pathway regulates MDR1/P-glycoproteinmediated multidrug resistance in colon carcinoma cells. World Chin J Digestol (Chin) 2011;19:892–898.

Sui H, Zhou LH, Liu X, Yin PH, Zhou N, Wang Y, et al. COX-2 egulate MDR1//P-glycoprotein-mediated multidrug resistance in colon carcinoma cells. Chin Oncol (Chin) 2011;21:241–246.

Xia W, Zhao T, Lv J, Xu S, Shi J, Wang S, et al. Celecoxib enhanced the sensitivity of cancer cells to anticancer drugs by inhibition of the expression of P-glycoprotein through a COX-2-independent manner. J Cell Biochem 2009;108:181–194.

Pan QC, Tian H. Reversal of multidrug resistance by various principles from Chinese herbal medicine. Chin Sci Bull 1995;40:1901–1905.

Engi H, Hohmann J, Gang G, Pusztai R, Rédei D, Kovács O, et al. Chemoprevention and inhibition of pglycoprotein in cancer cells by Chinese medicinal herbs. Phytother Res 2008;22:1671–1676.

Ye Y, Chou GX, Mu DD, Wang H, Chu JH, Leung AK, et al. Screening of Chinese herbal medicines for antityrosinase activity in a cell free system and B16 cells. J Ethnopharmacol 2010;129:387–390.

Duan P, Wang ZM. Clinical study on effect of Astragalus in efficacy enhancing and toxicity reducing of chemotherapy in patients of malignant tumor. Chin J Integr Med 2002;22:515–517.

Sui H, Li Q. Clinical research of traditional Chinese medicine on treatment of colorectal cancer. Liaoning J Tradit Chin Med (Chin) 2009;36:1241–1243.

Sui H, Li Q. Mechanism research of JNK/SAPK signal transduction pathway and multidrug resistance. Cancer Res prev treatment (Chin) 2010;37:844–847.

Barnes PM, Powell-Griner E, McFann K, Nahin RL. Complementary and alternative medicine use among adults: United States, 2002. Adv Data 2004;343:1–19.

Liao B, Ge RY, Chen X, Huangfu ZP, Qi Y, Song YP, et al. Synergistic reversal effect of Chinese medicine compound FFJZ combined with cyclosporine A on multidrug resistance of leukemia K562/VCR cell line. J Exp Hematol (Chin) 2007;15:752–755.

Xutian S, Zhang J, Louise W. New exploration and understanding of traditional Chinese medicine. Am J Chin Med 2009;37:411–426.

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