Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

Shokoofe Noori; Zuhair. M. Hassan; Vida Farsam

doi:10.1007/s11655-013-1454-2

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Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line

OriginalPaper | Updated：2021-08-27

- Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line
- Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line
- 中国结合医学杂志（英文版） 2014年20卷第8期页码：618-623
- Affiliations：
  
  1. Department of Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran
  2. Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran,Iran
- Author bio：
- Funds：
- DOI：10.1007/s11655-013-1454-2
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-6-15，
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Noori, S., Hassan, Z.M. & Farsam, V. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line., Chin. J. Integr. Med. 20, 618–623 (2014). https://doi.org/10.1007/s11655-013-1454-2

Shokoofe Noori, Zuhair. M. Hassan, Vida Farsam. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line[J]. Chinese Journal of Integrative Medicine, 2014,20(8):618-623.
Noori, S., Hassan, Z.M. & Farsam, V. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line., Chin. J. Integr. Med. 20, 618–623 (2014). https://doi.org/10.1007/s11655-013-1454-2 DOI：

Shokoofe Noori, Zuhair. M. Hassan, Vida Farsam. Artemisinin as a Chinese medicine, selectively induces apoptosis in pancreatic tumor cell line[J]. Chinese Journal of Integrative Medicine, 2014,20(8):618-623. DOI： 10.1007/s11655-013-1454-2.

摘要

To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. Column chromatography

thin layer chromatography (TLC) and proton NMR spectroscopy were used to purify Artemisinin. The flowcytometry was employed to detect apoptosis and reactive oxygen species (ROS). The results indicated that 50% inhibiting concentration (IC50 value) for pancreatic cell line (RIN) was 45 μmol/L of Artemisinin. Artemisinin had no cytotoxic effect on the growth of peripheral blood lymphocytes. The mechanism of apoptosis was evaluated by measuring intracellular ROS. It was shown that Artemisinin-induced apoptosis occurred independently of the binding of CD95L to CD95 receptor in the RIN cells. Moreover

Artemisinin

in a dose-dependent manner

could significantly increase the level of ROS. Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

Abstract

To investigate the possible mechanism through which Artemisinin induced apoptosis in pancreatic cell line. Column chromatography

Artemisinin

in a dose-dependent manner

could significantly increase the level of ROS. Artemisinin can induce apoptosis in the RIN cells via the generation of ROS and triggering the intrinsic pathway of cell death.

关键词

Artemisininendoperoxide bridgeapoptosisreactive oxygen species

Keywords

Artemisininendoperoxide bridgeapoptosisreactive oxygen species

references

Klayman DL. Qinghaosu (Artemisinin): an antimalarial drug from China. Science 1985;228:1049–1055.

Luo Z, Shen C. The chemistry, pharmacology, and clinical applications of qinghaosu (Artemisinin) and its derivatives. Med Res Rev 1987;7:29–52.

Borstnik K, Paik I, Posner G. Malaria: new chemotherapeutic peroxide drugs. Mini-Reviews in Medicinal Chemistry 2002;2:573–583.

Meshnick S, Yang Y, Lima V, Kuypers F, Kamchonwongpaisan S, Yuthavong Y. Iron-dependent free radical generation from the antimalarial agent Artemisinin (qinghaosu). Antimicrob Agents Chemother 1993;37:1108–1114.

O’Neill P, Posner G. A medicinal chemistry perspective on artemisinin and related endoperoxides. J Med Chem 2004;47:2945–2964.

Robert A, Coppel Y, Meunier B. A lkylation of heme by the antimalarial drug Artemisinin. Chem Commun 2002;5:414–415.

Sanjeev K, Anne-Catrin U, Richard KH. Artemisinins: mechanisms of action and potential for resistance. Drug Resistance Updates 2004;7:233–244.

Nathawut S, Rachanee U, Anake K, Udom C, Samlee M. Redox reaction of Artemisinin with ferrous and ferric ions in aqueous buffer. Chem Pharm Bull 2001;49:1541–1546.

Asawamahasakda W, Ittarat I, Pu Y, Ziffer H, Meshnick S. Reaction of antimalarial endoperoxides with specific parasite proteins. Antimicrob Agents Chemother 1994;38:1854–1858.

Thompson C. Apoptosis in the pathogenesis and treatment of disease. Science 1995;267:1456–1462.

Soini Y, Paakko P, Lehto V. Histopathological evaluation of apoptosis in cancer. Am J Pathol 1998;153:1041–1053.

Chick W, Warren S, Chute R, Like A, Lauris V, Kitchen K. A transplantable insulinoma in the rat. Proc Natl Acad Sci USA 1977;74:628–632.

Efferth T, Dunstan H, Sauerbrey A, Miyachi H, Chitambar C:The anti-malarial artesunate is also active against cancer. Int J Oncol 2001;18:767–773.

Efferth T, Davey M, Olbrich A, Rücher G, Gebbart E, Daveu R. Activity of drugs from traditional Chinese medicine towards sensitive and MDRI- or MRPI-overexpressing multidrug-resistant human CCRF-CEM leukemia cells. Blood Cells Mol Dis 2002;28:160–168.

Berman P, Adams P. Artemisinin enhances hemecatalysed oxidation of lipid membranes. Free Radic Bio Med 1997;22:1283–1288.

Zhang F, Grosser D, Meshnick S. Hemin-catalyzed decomposition of Artemisinin (qinghaosu). Biochem Pharmacol 1992;43:1805–1809.

Singh NP, Lai H. Artemisinin induces apoptosis in human cancer cells. Anticancer Research 2004;24:2277–2280.

Kihara C, Tsunoda T, Tanaka T, Yamana H, Furukawa Y, Ono K, et al. Prediction of sensitivity of esophageal tumor to adjuvant chemotherapy by cDNA microarray analysis of gene-expression profiles. Cancer Res 2001;61:6474–6479.

Singh N, Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sci 2001;70:49–56.

Lai H, Singh N. Selective cancer cell cytotoxicity from exposure to dihydro artemisinin and holotransferrin. Cancer Lett 1995;91:41–46.

Price R, van Vugt M, Nosten F, Luxemburger C, Brockman A, Phaipun L, et al. Artesunate versus artemether for the treatment of recrudescent multidrug resistant plasmodium falciparum malaria. Am J Trop Med Hyg 1998;59:883–888.

Efferth T, Briehl M, Tome M. Role of antioxidant genes for the activity of artesunate against tumor cells. Int J Oncol 2003;23:1231–1235.

Meshnick S. Artemisinin: mechanisms of action, resistance and toxicity. Int J Parasitol 2002;32:1655–1660.

Fleury C, Mignotte B, Vayssiere J. Mitochondrial reactive oxygen species in cell death signaling. Biochimie 2002;84:131–141.

Li Y, Wu YL. How Chinese scientists discovered qinghaosu (Artemisinin) and developed its derivatives. What are the future perspectives? Med Trop 1998, 58:9–12.

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