Protective effects of Sapindus saponins in spontaneously hypertensive rats

Ming Chen; Zhi-wu Chen; Zi-jiang Long; Ju-tao Wang; Ya-juan Wang; Jin-lin Liu

doi:10.1007/s11655-013-1464-0

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Protective effects of Sapindus saponins in spontaneously hypertensive rats

OriginalPaper | Updated：2021-08-27

- Protective effects of Sapindus saponins in spontaneously hypertensive rats
- Protective effects of Sapindus saponins in spontaneously hypertensive rats
- 中国结合医学杂志（英文版） 2015年21卷第1期页码：36-42
- Affiliations：
  
  1. Department of Pharmacology, Anhui Medical University,Hefei,China
  2. Department of Pharmacology, Anhui University of Traditional Chinese Medicine,Hefei,China
  3. Anqing Medical and Pharmaceutical College,Anhui Province,Anqing,China
- Author bio：
- Funds：
  
  Supported by the Foundation for Young Talents in College of Anhui Province under Grant (No. 2009SQRZ114) and Youth Scientific Research Foundation of Anhui University of Traditional Chinese Medicine (No. 2012QN005).
- DOI：10.1007/s11655-013-1464-0
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2013-6-15，
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Chen, M., Chen, Zw., Long, Zj. et al. Protective effects of Sapindus saponins in spontaneously hypertensive rats., Chin. J. Integr. Med. 21, 36–42 (2015). https://doi.org/10.1007/s11655-013-1464-0

Ming Chen, Zhi-wu Chen, Zi-jiang Long, et al. Protective effects of Sapindus saponins in spontaneously hypertensive rats[J]. Chinese Journal of Integrative Medicine, 2015,21(1):36-42.
Chen, M., Chen, Zw., Long, Zj. et al. Protective effects of Sapindus saponins in spontaneously hypertensive rats., Chin. J. Integr. Med. 21, 36–42 (2015). https://doi.org/10.1007/s11655-013-1464-0 DOI：

Ming Chen, Zhi-wu Chen, Zi-jiang Long, et al. Protective effects of Sapindus saponins in spontaneously hypertensive rats[J]. Chinese Journal of Integrative Medicine, 2015,21(1):36-42. DOI： 10.1007/s11655-013-1464-0.

摘要

To investigate the protective effects of Sapindus saponins in spontaneously hypertensive rats

and the possible cellular and molecular mechanisms. Thirty-two 16-week-old spontaneously hypertensive rats were randomly divided into four groups (8 in each group): model group (placebo)

positive control group (27 mg/kg of Captopril Tablets)

Sapindus saponins groups (27 mg/kg and 108 mg/kg

respectively). Another 8 healthy Wistar-Kyoto strain (WKY) rats were used as the normal group. The animals were treated for 8 weeks. Blood pressure of rats was determined by non-invasive blood pressure meter (BP-6). Furthermore

the contents of angiotensin II (Ang II) in plasma and myocardial tissue were determined by enzyme-linked immunosorbent assay (ELISA)

the gene expression of receptor angiotensin type 1 (AT1R) in aorta was determined by quantitative realtime polymerase chain reaction (qRT-PCR). The protein expression of transforming growth factor-β1 (TGF-β1) and AT1R in heart was determined by immunohistochemical staining. The protein expression of p-phosphorylation of p38 mitogen-activated protein kinase (p-p38MAPK) was determined by Western blotting. The contents of interleukin (IL)-1

IL-6 and tumor necrosis factor (TNF) in serum were determined by radioimmunoassay. And the histopathological and morphological changes of aorta and heart tissue samples were assessed semi-quantitatively by hematoxylin-eosin (HE) or Masson staining. Thirty minutes after single or continuous treatment

systolic blood pressure (SBP) was reduced significantly in Sapindus saponins groups. And the contents of AngII

IL-1

IL-6 and TNF-α in serum

the expression of AT1R mRNA

p-p38MAPK and TGF-β1 were significantly suppressed dose-dependently (P<0.05 or P<0.01). With the Sapindus saponins treatment

compared with those of the model group

the cardiac and aortic pathological changes were ameliorated significantly. Our findings suggest that Sapindus saponins might have protective effects in spontaneously hypertensive rats

the cellular and molecular mechanisms of which might be relevant to the regulation of inflammatory responses mediated by p-p38MAPK signal pathway based on activated Ang II and AT1R.

Abstract

To investigate the protective effects of Sapindus saponins in spontaneously hypertensive rats

and the possible cellular and molecular mechanisms. Thirty-two 16-week-old spontaneously hypertensive rats were randomly divided into four groups (8 in each group): model group (placebo)

positive control group (27 mg/kg of Captopril Tablets)

Sapindus saponins groups (27 mg/kg and 108 mg/kg

the contents of angiotensin II (Ang II) in plasma and myocardial tissue were determined by enzyme-linked immunosorbent assay (ELISA)

systolic blood pressure (SBP) was reduced significantly in Sapindus saponins groups. And the contents of AngII

IL-1

IL-6 and TNF-α in serum

the expression of AT1R mRNA

p-p38MAPK and TGF-β1 were significantly suppressed dose-dependently (P<0.05 or P<0.01). With the Sapindus saponins treatment

compared with those of the model group

the cardiac and aortic pathological changes were ameliorated significantly. Our findings suggest that Sapindus saponins might have protective effects in spontaneously hypertensive rats

the cellular and molecular mechanisms of which might be relevant to the regulation of inflammatory responses mediated by p-p38MAPK signal pathway based on activated Ang II and AT1R.

关键词

Sapindus saponinshypertensionrenin-angiotensin-aldosterone systemphosphorylation of p38 mitogen-activated protein kinase pathway (p-p38 MAPK)

Keywords

Sapindus saponinshypertensionrenin-angiotensin-aldosterone systemphosphorylation of p38 mitogen-activated protein kinase pathway (p-p38 MAPK)

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