Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration

Pu-yan Wang; Xiao-chun Liang; Hong Zhang; Li Zhao; Qing Sun; Wen-zhi Huang; Bo-wu Li

doi:10.1007/s11655-013-1506-7

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Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration

OriginalPaper | Updated：2021-08-27

- Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration
- Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration
- 中国结合医学杂志（英文版） 2013年19卷第7期页码：517-523
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences,Beijing,China
  2. Institute of Basic Medical Science, Peking Union Medical College, Chinese Academy of Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by Beijing Natural Science Foundation (No. 7082077);The author is now working in the Department of Traditional Chinese Medicine, 306th Hospital of People’s Liberation Army, Beijing (100101), China
- DOI：10.1007/s11655-013-1506-7
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2013-7-2，
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Wang, Py., Liang, Xc., Zhang, H. et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration., Chin. J. Integr. Med. 19, 517–523 (2013). https://doi.org/10.1007/s11655-013-1506-7

Pu-yan Wang, Xiao-chun Liang, Hong Zhang, et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration[J]. Chinese Journal of Integrative Medicine, 2013,19(7):517-523.
Wang, Py., Liang, Xc., Zhang, H. et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration., Chin. J. Integr. Med. 19, 517–523 (2013). https://doi.org/10.1007/s11655-013-1506-7 DOI：

Pu-yan Wang, Xiao-chun Liang, Hong Zhang, et al. Effect of serum containing Jinmaitong Capsule (筋脉通胶囊) on Rats’ Schwann cell apoptosis induced by high glucose concentration[J]. Chinese Journal of Integrative Medicine, 2013,19(7):517-523. DOI： 10.1007/s11655-013-1506-7.

摘要

To evaluate the effect of serum containing Jinmaitong Capsule (筋脉通胶囊

JMT) on apoptosis of Schwann cells (SCs) that are cultured in high glucose at the cellular and molecular levels. SCs were cultured in Dulbecco’s modified Eagle’s medium (control group)

high glucose (50 mmol/L) medium supplemented with 20% rat serum (HG group)

and 50 mmol/L glucose medium supplemented with serum containing JMT (JMT group). SC apoptosis was detected using a terminal deoxynucleotidyl transferase dUTP nick end labeling kit. The expression of Bcl-2 and the caspase-3 p20 subunit in SCs were detected by realtime fluorogenic quantitative polymerase chain reaction and confocal laser scanning microscopy

respectively. No apoptosis was detected in SCs that were cultured in the control group. The percentage of apoptosis of SCs cultured in the HG group was much higher than that in the control group. The apoptosis of SCs in the JMT group was lower than that in the HG group. Fluorescence intensity of Bcl-2 and the expression of Bcl-2 mRNA in SCs that were cultured in the HG group were much lower than those in the control group and much higher than those in the JMT group (P<0.01). The fluorescence intensity of caspase-3 p20 and the expression of caspase-3 p20 mRNA in SCs that were cultured in the HG group were much higher than those in the control group (P<0.01)

and they were remarkably lower in the JMT group (P<0.01). JMT effectively prevents SC apoptosis that is induced by high glucose. This effect may be because of increased expression of Bcl-2 mRNA and protein and decreased expression of caspase-3 p20 mRNA and protein.

Abstract

To evaluate the effect of serum containing Jinmaitong Capsule (筋脉通胶囊

JMT) on apoptosis of Schwann cells (SCs) that are cultured in high glucose at the cellular and molecular levels. SCs were cultured in Dulbecco’s modified Eagle’s medium (control group)

high glucose (50 mmol/L) medium supplemented with 20% rat serum (HG group)

关键词

Jinmaitong Capsuleapoptosishigh glucoseSchwann cells

Keywords

Jinmaitong Capsuleapoptosishigh glucoseSchwann cells

references

Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: mechanisms to management. Pharmacol Ther 2008;120:1–34.

Investigation Group for Chronic Diabetic Complications. A nationwide retrospective analysis on chronic diabetic complications and related macrovascular diseases of inpatient with diabetes during 1991–2000. Acta Acad Med Sin 2002;24:447–451.

Liang XC, Cui LY, Guo SS, Zhang KJ, Hao WX, Jia L, et al. Clinical study on Jinmaitong Composita on diabetic peripheral neuropathy. Chin J Integr Med 2001;7:103–106.

Qu L, Liang XC, Zhang H, Wu QL, Sun LQ, Gu P. Effect of Jinmaitong with medicated serum on proliferation of rat SCs cultured in high glucose medium. Chin Integr Med 2008;14:293–297.

Mathew CE, Berdanier CD. Noninsulin-dependent diabetes mellitus as a mitochondrial genomic disease. Proc Soc Exper Biol Med 1998;219:97–108.

Schapira AH. Mitochondrial dysfunction in neurodegenerative disorders. Biochim Biophys Acta 1998;1366:225–233.

Brownlee M. Biochemistry and molecular cell of diabetic complications. Nature 2001;414:813–820.

Peng YQ, Ji YL, Jiang ZP, Yu XQ, Du Y. Effects of inhibition of NAD(P)H oxidase expression and activity in diabetic nephropathy. J Sun Yat-sen Univ Med Sci (Chin) 2004;25:245–248,263.

Han Z, Hendrickson EA, Bremner TA, Wyche JH. A sequential two-step mechanism for the production of the mature p17:p12 form of caspase-3 in vitro. J Biological Chem 1997;272:13432–13436.

Coppey LJ, Gellett JS, Davidson EP, Yorek MA. Preventing superoxide formation in epineurial arterioles of the sciatic nerve from diabetic rats restores endothelium-dependent vasodilation. Free Radic Res 2003;37:33–40.

Brownlee M. The pathobiology of diabetic complications: a unifying methanism. Diabeties 2005;54:1615–1625.

Jakobsen J, Lundbak K. Neuropathy in experimental diabetes: an animal model. Br Med J 1976;31(6030):278–279.

Chokroverty S, Seiden D, Navidad P, Cody R. Distal axonopathy in streptozotocin diabetes in rats. Experientia 1988;44:444–446.

Forman LJ, Estilow S, Lewis M, Lewis M, Vasilenko P. Streptozotocin diabetes alters immunoreactive β-endorphin levels and pain perception after 8wk in female rats. Diabetes 1986;35:1309–1313.

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