Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo

Yue-long Cao; Ting Liu; Jian Pang; Ning-yang Gao; Hong-sheng Zhan; Yin-yu Shi; Xiang Wang; Shun-chun Wang

doi:10.1007/s11655-013-1511-x

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Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo

OriginalPaper | Updated：2021-08-27

- Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo
- Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo
- 中国结合医学杂志（英文版） 2015年21卷第3期页码：196-203
- Affiliations：
  
  1. Research Institute of Orthopaedics, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Institute of Chinese Materia Medica of Shanghai University of Traditional Chinese Medicine,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 30300459, 81072830), Shanghai Leading Academic Discipline Project (No. T0303) and the Shanghai Youth Phospherus Project (No. 08QA14063)
- DOI：10.1007/s11655-013-1511-x
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2013-7-16，
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Cao, Yl., Liu, T., Pang, J. et al. Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo, ., Chin. J. Integr. Med. 21, 196–203 (2015). https://doi.org/10.1007/s11655-013-1511-x

Yue-long Cao, Ting Liu, Jian Pang, et al. Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo[J]. Chinese Journal of Integrative Medicine, 2015,21(3):196-203.
Cao, Yl., Liu, T., Pang, J. et al. Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo, ., Chin. J. Integr. Med. 21, 196–203 (2015). https://doi.org/10.1007/s11655-013-1511-x DOI：

Yue-long Cao, Ting Liu, Jian Pang, et al. Glucan HBP-A increase type II collagen expression of chondrocytes in vitro and tissue engineered cartilage in vivo[J]. Chinese Journal of Integrative Medicine, 2015,21(3):196-203. DOI： 10.1007/s11655-013-1511-x.

摘要

Although chondroprotective activities have been documented for polysaccharides

the potential target of different polysaccharide may differ. The study was aimed to explore the effect of glucan HBP-A in chondrocyte monolayer culture and chondrocytes-alginate hydrogel constructs in vivo

especially on the expression of type II collagen. Chondrocytes isolated from rabbit articular cartilage were cultured and verified by immunocytochemical staining of type II collagen. Chondrocyte viability was assessed after being treated with HBP-A in different concentrations. Morphological status of chondrocytes-alginate hydrogel constructs in vitro was observed by scanning electron microscope (SEM). The constructs were treated with HBP-A and then injected to nude mice subcutaneously. Six weeks after transplantation

the specimens were observed through transmission electron microscopy (TEM). The mRNA expressions of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5)

aggrecan and type II collagen in both monolayer culture and constructs were determined by real time polymerase chain reaction (PCR). The expression of type II collagen and matrix metalloproteinases-3 (MMP-3) in chondrocyte monolayer culture was also tested through Western blot and enzyme linked immunosorbent assay (ELISA)

respectively. MMP-3 secretion and ADAMTs-5 mRNA expression in vitro were inhibited by HBP-A at 0.3 mg/mL concentration. In morphological study

there were significant appearance of collagen in those constructs treated by HBP-A. Accordingly

in both chondrocyte monolayer culture and chondrocytes-alginate hydrogel constructs

the expression of type II collagen was increased significantly in HBP-A group when compared with control group (P<0.001). The study documented that the potential pharmacological target of glucan HBP-A in chondrocytes monolayer culture and tissue engineered cartilage in vivo may be concerned with the inhibition of catabolic enzymes MMP-3

ADAMTs-5

and increasing of type II collagen expression.

Abstract

Although chondroprotective activities have been documented for polysaccharides

the specimens were observed through transmission electron microscopy (TEM). The mRNA expressions of disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTs-5)

respectively. MMP-3 secretion and ADAMTs-5 mRNA expression in vitro were inhibited by HBP-A at 0.3 mg/mL concentration. In morphological study

there were significant appearance of collagen in those constructs treated by HBP-A. Accordingly

in both chondrocyte monolayer culture and chondrocytes-alginate hydrogel constructs

ADAMTs-5

and increasing of type II collagen expression.

关键词

Chinese Medicineglucan HBP-Aosteoarthritischondrocytecollagen II

Keywords

Chinese Medicineglucan HBP-Aosteoarthritischondrocytecollagen II

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