Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways

Ye Liu; Ling-li Shao; Wei Pang; Xiao-mei Lan; Jian-xin Lu; Yu-long Cong; Cheng-bin Wang

doi:10.1007/s11655-013-1515-6

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Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways

OriginalPaper | Updated：2021-08-27

- Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways
- Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways
- 中国结合医学杂志（英文版） 2014年20卷第5期页码：360-368
- Affiliations：
  
  1. Department of Clinical Laboratory, Chinese People’s Liberation Army General Hospital,Beijing,China
  2. Deparment of Laboratory Medicine, Wenzhou Medical College,Zhejiang Province,Wenzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Sciences Foundation of China (No. 30873419);These authors contributed equally to this work
- DOI：10.1007/s11655-013-1515-6
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-10-10，
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Liu, Y., Shao, Ll., Pang, W. et al. Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways., Chin. J. Integr. Med. 20, 360–368 (2014). https://doi.org/10.1007/s11655-013-1515-6

Ye Liu, Ling-li Shao, Wei Pang, et al. Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways[J]. Chinese Journal of Integrative Medicine, 2014,20(5):360-368.
Liu, Y., Shao, Ll., Pang, W. et al. Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways., Chin. J. Integr. Med. 20, 360–368 (2014). https://doi.org/10.1007/s11655-013-1515-6 DOI：

Ye Liu, Ling-li Shao, Wei Pang, et al. Induction of adhesion molecule expression in co-culture of human bronchial epithelial cells and neutrophils suppressed by puerarin via down-regulating p38 mitogen-activated protein kinase and nuclear factor κB pathways[J]. Chinese Journal of Integrative Medicine, 2014,20(5):360-368. DOI： 10.1007/s11655-013-1515-6.

摘要

In this study

we aimed to investigate the expressions of adhesion molecules on human bronchial epithelial cells and neutrophils in co-culture system

assess the effects of puerarin on suppressing these adhesion molecules expressions

and explore the roles of two crucial signal-transduction elements p38 mitogen-activated protein kinase (p38 MAPK) and nuclear factor kappa B (NF-κB) in modulating adhesion molecules expressions. Neutrophils and BEAS-2B cells (one human bronchial epithelial cell line) were co-cultured

and adhesion molecules expressions on cell surface were detected using flow cytometry. The mRNA levels of adhesion molecules were assessed by real-time quantitative polymerase chain reaction (real-time qPCR). Phosphorylated p38 MAPK and inhibitor κB were analyzed by Western blot. In co-culture system

adhesion molecules expressions on BEAS-2B cells and neutrophils were enhanced significantly (P<0.05). Correspondingly

the mRNA levels of adhesion molecules were also increased greatly. Moreover

the pretreatment of peurarin obviously suppressed adhesion molecules expressions on cell surface. Furthermore

phosphorylated p38 MAPK and inhibitor κB in BEAS-2B cells and neutrophils were elevated in co-culture system

but decreased significantly after upon the treatment of peurarin (P<0.05). Coculture boosted the interactions between human bronchial epithelial cells and neutrophils mimicking airway inflflammation

whereas peurarin decreased the expression of adhesion molecules on cell surface by suppressing the activities of p38 MAPK and NF-κB pathways

and exhibiting its anti-inflflammation activity.

Abstract