Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines

Shu-mei Wang; Li-zhu Lin; Dai-han Zhou; Jing-xu Zhou; Shao-quan Xiong

doi:10.1007/s11655-013-1535-2

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Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines

OriginalPaper | Updated：2021-08-27

- Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines
- Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines
- 中国结合医学杂志（英文版） 2015年21卷第9期页码：689-696
- Affiliations：
  
  1. Department of Clinical Teaching and Research, College of Traditional Chinese Medicine, Chongqing Medical University,Chongqing,China
  2. Department of Oncology, the First Affiliated Hospital of Guangzhou Traditional Chinese Medicine University,Guangzhou,China
  3. Department of Oncology, the Affiliated Hospital of Chengdu Traditional Chinese Medicine University,Chengdu,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Fundation of China (No. 30772862);Guangdong Natural Science Fund (No. 2010-3);Project of Guangdong University Talent Fund (No. 2010-79)
- DOI：10.1007/s11655-013-1535-2
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-1-2，
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Wang, Sm., Lin, Lz., Zhou, Dh. et al. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines., Chin. J. Integr. Med. 21, 689–696 (2015). https://doi.org/10.1007/s11655-013-1535-2

Shu-mei Wang, Li-zhu Lin, Dai-han Zhou, et al. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines[J]. Chinese Journal of Integrative Medicine, 2015,21(9):689-696.
Wang, Sm., Lin, Lz., Zhou, Dh. et al. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines., Chin. J. Integr. Med. 21, 689–696 (2015). https://doi.org/10.1007/s11655-013-1535-2 DOI：

Shu-mei Wang, Li-zhu Lin, Dai-han Zhou, et al. Expression of prolyl 4-hydroxylase beta-polypeptide in non-small cell lung cancer treated with Chinese medicines[J]. Chinese Journal of Integrative Medicine, 2015,21(9):689-696. DOI： 10.1007/s11655-013-1535-2.

摘要

To investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula (益气除痰方

YQCTF). Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice. The differential expression of P4HB protein between the YQCTF (3.0 g/kg

gavage

once daily

21 days) group and the control group was acquired by a 2 fluorescence difference gel electrophoresis (2D-DIGE)

verified by Western blotting and identified by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS). The expression of P4HB and P4HB mRNA in cultured A549 cells from cisplatin (DDP) 1.5 µg/mL group and 15% serum combined with DDP 1.5 µg/mL group were detected by cellular immunohistochemistry and reverse transcription-polymerase chain reaction

respectively. The proteomics research discovered that one-third of differential proteins including P4HB were decreased in the YQCTF group (P<0.01). Clinical pathology and tissue microarray studies showed that P4HB expression in lung cancer tissue was stronger than adjacent tissues and normal lung epithelial (P<0.01). In the YQCTF and DDP combined groups

the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly (P<0.01). YQCTF could inhibit the LEWIS lung carcinoma's growth

decrease the expression of P4HB in LEWIS lung carcinoma and A549 cells. YQCTF might take effect through regulating P4HB in endoplasmic reticulum to inhibit the incidence and growth process of lung carcinoma.

Abstract

To investigate the role of prolyl 4-hydroxylase beta polypeptide (P4HB) expressed in lung carcinoma and the intervention effect of Yiqi Chutan Formula (益气除痰方

YQCTF). Lung carcinoma model was established by subcutaneously inoculating LEWIS lung carcinoma cells in C57BL/6J mice. The differential expression of P4HB protein between the YQCTF (3.0 g/kg

gavage

once daily

21 days) group and the control group was acquired by a 2 fluorescence difference gel electrophoresis (2D-DIGE)

the expression of P4HB and P4HB mRNA in A549 cell were decreased significantly (P<0.01). YQCTF could inhibit the LEWIS lung carcinoma's growth

关键词

Yiqi Chutan Formulaprolyl 4-hydroxylase beta polypeptideunfolded protein responseChinese Medicine

Keywords

Yiqi Chutan Formulaprolyl 4-hydroxylase beta polypeptideunfolded protein responseChinese Medicine

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