The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

Hai-lian Shi; Bao Tan; Guang Ji; Lan Lu; Jian-qun Xie

doi:10.1007/s11655-013-1536-1

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The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells

OriginalPaper | Updated：2021-08-27

- The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells
- The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells
- 中国结合医学杂志（英文版） 2014年20卷第6期页码：430-437
- Affiliations：
  
  1. Institute of Digestive Disease, Longhua Hospital, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  2. Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine,Shanghai,China
  3. Shanghai Key Laboratory of Complex Prescription,Shanghai,China
  4. Chinese Medicine Hospital of Shanxi Province,Taiyuan,China
- Author bio：
- Funds：
  
  Supported by High Level Project of University of Educational Commission of Shanghai, Shanghai, China (No. 2008GSP19);Shanghai Municipal Natural Science Foundation, Shanghai China (09ZR1431800);Opening Project of Shanghai Key Laboratory of Complex Prescription, Shanghai, China (No. 11DZ2272300);Educational Commission of Shanghai, Shanghai, China (No. 09JW21 and No. 2012JW19)
- DOI：10.1007/s11655-013-1536-1
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-8-13，
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Shi, Hl., Tan, B., Ji, G. et al. The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells., Chin. J. Integr. Med. 20, 430–437 (2014). https://doi.org/10.1007/s11655-013-1536-1

Hai-lian Shi, Bao Tan, Guang Ji, et al. The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells[J]. Chinese Journal of Integrative Medicine, 2014,20(6):430-437.
Shi, Hl., Tan, B., Ji, G. et al. The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells., Chin. J. Integr. Med. 20, 430–437 (2014). https://doi.org/10.1007/s11655-013-1536-1 DOI：

Hai-lian Shi, Bao Tan, Guang Ji, et al. The ethanol extract isolated from Weiqi Decoction (胃祺饮) induces G2/M arrest and apoptosis in AGS cells[J]. Chinese Journal of Integrative Medicine, 2014,20(6):430-437. DOI： 10.1007/s11655-013-1536-1.

摘要

To evaluate the effects of the ethanol extract isolated from Weiqi Decoction (胃祺饮

WQD-EE) on AGS cell proliferation and apoptosis. By using high-performance liquid chromatography with ultraviolet detectors (HPLC-UV) assay and MTT method

the main compounds in WQD-EE and cell viability were detected. And cell cycle distributions were determined by flow cytometry with propidium iodine (PI) staining while apoptosis was detected by flow cytometry with annexin V/PI double staining. Finally

caspase-3 activities were measured by colorimetric method and protein expression was determined by Western blotting. HPLC analysis showed that naringin (35.92 μg/mg)

nobiletin (21.98 μg/mg)

neohesperidin (17.98 μg/mg) and tangeretin (0.756 μg/mg) may be the main compounds in WQD-EE. WQD-EE not only inhibited AGS and MCF 7 cell proliferation in a dose-dependent manner

but also blocked cell cycle progression at G2/M stage as well as inducing cell apoptosis at concentrations triggering significant inhibition of proliferation and cell cycle arrest in AGS cells. While at 0.5 mg/mL

WQD-EE significantly increased caspase-3 activity by 2.75 and 7.47 times at 24 h and 48 h

respectively. Moreover

WQD-EE in one hand reduced protein expressions of p53 and cyclin B1

and in other hand enhanced protein expressions of cytochrome c and Bax. Protein levels of Bcl-2

Fas L and Fas were not significantly affected by WQD-EE. WQD-EE inhibits AGS cell proliferation through G2/M arrest due to down-regulation of cyclin B1 protein expression

and promotes apoptosis by caspase-3 and mitochondria-dependent pathways

but not by p53-dependent pathway.

Abstract

To evaluate the effects of the ethanol extract isolated from Weiqi Decoction (胃祺饮

WQD-EE) on AGS cell proliferation and apoptosis. By using high-performance liquid chromatography with ultraviolet detectors (HPLC-UV) assay and MTT method

caspase-3 activities were measured by colorimetric method and protein expression was determined by Western blotting. HPLC analysis showed that naringin (35.92 μg/mg)

nobiletin (21.98 μg/mg)

neohesperidin (17.98 μg/mg) and tangeretin (0.756 μg/mg) may be the main compounds in WQD-EE. WQD-EE not only inhibited AGS and MCF 7 cell proliferation in a dose-dependent manner

WQD-EE significantly increased caspase-3 activity by 2.75 and 7.47 times at 24 h and 48 h

respectively. Moreover

WQD-EE in one hand reduced protein expressions of p53 and cyclin B1

and in other hand enhanced protein expressions of cytochrome c and Bax. Protein levels of Bcl-2

Fas L and Fas were not significantly affected by WQD-EE. WQD-EE inhibits AGS cell proliferation through G2/M arrest due to down-regulation of cyclin B1 protein expression

and promotes apoptosis by caspase-3 and mitochondria-dependent pathways

but not by p53-dependent pathway.

关键词

Weiqi DecoctionproliferationapoptosisAGS cellsChinese Medicine

Keywords

Weiqi DecoctionproliferationapoptosisAGS cellsChinese Medicine

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