Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo

Xue-jin Zhang; Xiao-feng Xu; Rui-lan Gao; Jian-feng Xu

doi:10.1007/s11655-013-1537-0

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Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo

OriginalPaper | Updated：2021-08-27

- Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo
- Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo
- 中国结合医学杂志（英文版） 2014年20卷第1期页码：36-42
- Affiliations：
  
  1. Division of Hematology, Hangzhou Red Cross Hospital,Hangzhou,China
  2. Institute of Hematology, Chinese Medicine Hospital of Zhejiang Province,Hangzhou,China
  3. Arkansas Biosciences Institute, College of Agriculture and Technology, Arkansas State University, Jonesboro,USA
- Author bio：
- Funds：
  
  Supported by Provincial Administration of Traditional Chinese Medicine of Zhejiang Province, China (No. 2008CB063)
- DOI：10.1007/s11655-013-1537-0
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-11-16，
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Zhang, Xj., Xu, Xf., Gao, Rl. et al. , Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo, ., Chin. J. Integr. Med. 20, 36–42 (2014). https://doi.org/10.1007/s11655-013-1537-0

Xue-jin Zhang, Xiao-feng Xu, Rui-lan Gao, et al. Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo[J]. Chinese Journal of Integrative Medicine, 2014,20(1):36-42.
Zhang, Xj., Xu, Xf., Gao, Rl. et al. , Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo, ., Chin. J. Integr. Med. 20, 36–42 (2014). https://doi.org/10.1007/s11655-013-1537-0 DOI：

Xue-jin Zhang, Xiao-feng Xu, Rui-lan Gao, et al. Rubus parvifolius L. inhibited the growth of leukemia K562 cells in vitro and in vivo[J]. Chinese Journal of Integrative Medicine, 2014,20(1):36-42. DOI： 10.1007/s11655-013-1537-0.

摘要

To determine the antiproliferative activity of Rubus parvifolius L. (RP) extract

its medicinal serum and RP total saponins (RPTS) against K562 cells in vitro and in vivo. Nude mice models bearing leukemia tumors were treated with different concentrations of RP extract. The size

weight and histopathological change of leukemic tumors were determined. Semi-solid agar culture and methylthiazolyl tetrazolium (MTT) assay were used to determine in vitro the inhibition of colony formation and proliferation of K562 cells respectively by different concentrations of RP medicinal serum and RPTS. RP extract had a tumor inhibition rate of 84.8% when administered to mice at a dose of 1.0 g/day of crude RP root equivalent. Semi-solid agar culture of K562 cells in the presence of 20% (v/v) of RP medicinal serum and 150 mg/L RPTS demonstrated a 50.8% and 100% inhibition of the colony forming unit (CFU)-K562

respectively. The same doses of RP medicinal serum and RPTS showed a proliferation inhibition of 31.4% and 86.3%

respectively against K562 cells in MTT assay. RP extract and RPTS show effective antiproliferative activity against myeloid leukemia cells in vitro and in vivo.

Abstract

To determine the antiproliferative activity of Rubus parvifolius L. (RP) extract

its medicinal serum and RP total saponins (RPTS) against K562 cells in vitro and in vivo. Nude mice models bearing leukemia tumors were treated with different concentrations of RP extract. The size

respectively. The same doses of RP medicinal serum and RPTS showed a proliferation inhibition of 31.4% and 86.3%

respectively against K562 cells in MTT assay. RP extract and RPTS show effective antiproliferative activity against myeloid leukemia cells in vitro and in vivo.

关键词

Rubus parvifolius L.medicinal serumtotal saponinsleukemic animal modelK562 CellinhibitionChinese Medicine

Keywords

Rubus parvifolius L.medicinal serumtotal saponinsleukemic animal modelK562 CellinhibitionChinese Medicine

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