The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice

Wei-hong Li; Qi-jie Li; Wan-zhen Li; Wei-wei Liu; Ying-zi Zeng; Tian-e Zhang; Xian-geng Zhang; Su-qin Sun; Mi-qu Wang; Wei-jun Ding

doi:10.1007/s11655-013-1542-3

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The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice

OriginalPaper | Updated：2021-08-27

- The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice
- The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice
- 中国结合医学杂志（英文版） 2014年20卷第11期页码：829-834
- Affiliations：
  
  Department of Fundamental Medicine, Chengdu University of Traditional Chinese Medicine,Chengdu,China
- Author bio：
- Funds：
  
  Supported by the National Natural Sciences Foundation of China (No. 30171126 and 30873316)
- DOI：10.1007/s11655-013-1542-3
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-12-13，
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Li, Wh., Li, Qj., Li, Wz. et al. The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice., Chin. J. Integr. Med. 20, 829–834 (2014). https://doi.org/10.1007/s11655-013-1542-3

Wei-hong Li, Qi-jie Li, Wan-zhen Li, et al. The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice[J]. Chinese Journal of Integrative Medicine, 2014,20(11):829-834.
Li, Wh., Li, Qj., Li, Wz. et al. The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice., Chin. J. Integr. Med. 20, 829–834 (2014). https://doi.org/10.1007/s11655-013-1542-3 DOI：

Wei-hong Li, Qi-jie Li, Wan-zhen Li, et al. The Fourier transform infrared spectra of the key organs derived from Kidney (Shen)-yang deficiency syndrome mice[J]. Chinese Journal of Integrative Medicine, 2014,20(11):829-834. DOI： 10.1007/s11655-013-1542-3.

摘要

To explore an approach to rapidly and accurately identify the compounds as biomarkers of Chinese medicine (CM) syndromes. The Fourier transform infrared (FT-IR) spectrometry was applied to investigate the characteristic components of a mice model of Kidney (Shen)-yang deficiency syndrome (KDS)

and the remedial effect of a typical CM formula Shenqi Pill (肾气丸). Thirty-six females and 18 males of Balb/c mice were randomly divided into KDS

Shenqi or control group. The females and males of the same group freely were mated for 96 h

and the males were taken out and only the female mice were raised. Females of the KDS group were threatened by a ferocious cat every other day for 14 d. After delivery

the KDS

or gestational threatened

offspring were raised at standard condition for 11 weeks. Then 10 male offspring were randomly selected

anaesthetized and their representative organs

i.e. testes

kidneys

lungs and feet were collected

for the FT-IR scan. Mice of the Shenqi group were intragastric administered Shenqi Pill; while mice in the KDS and control groups were given the same volume of saline. The attenuated birth outcomes of the KDS group were displayed. The remarkable FT-IR differences of all organs between KDS mice and healthy control were mainly at 1

735–1

745 cm−1 (indicating the increased levels of lipids) and at 1

640–1

647 cm−1 and 1

539–1

544 cm−1 (displaying the decreased proteins). No statistic FT-IR difference between Shenqi and control mice was observed. In accordance with major traits of KDS

prenatal stress extensively impaired the building up of proteins and resulting in the excessive lipid storage

and FT-IR could effectively identify the biomarkers of KDS.

Abstract

and the remedial effect of a typical CM formula Shenqi Pill (肾气丸). Thirty-six females and 18 males of Balb/c mice were randomly divided into KDS

Shenqi or control group. The females and males of the same group freely were mated for 96 h

and the males were taken out and only the female mice were raised. Females of the KDS group were threatened by a ferocious cat every other day for 14 d. After delivery

the KDS

or gestational threatened

offspring were raised at standard condition for 11 weeks. Then 10 male offspring were randomly selected

anaesthetized and their representative organs

i.e. testes

kidneys

lungs and feet were collected

735–1

745 cm−1 (indicating the increased levels of lipids) and at 1

640–1

647 cm−1 and 1

539–1

544 cm−1 (displaying the decreased proteins). No statistic FT-IR difference between Shenqi and control mice was observed. In accordance with major traits of KDS

prenatal stress extensively impaired the building up of proteins and resulting in the excessive lipid storage

and FT-IR could effectively identify the biomarkers of KDS.

关键词

Fourier-transform infraredprenatal stressChinese MedicineKidney (Shen)-yang deficiency syndrome

Keywords

Fourier-transform infraredprenatal stressChinese MedicineKidney (Shen)-yang deficiency syndrome

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