Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats

Hong Cao; Jin-wei Zheng; Jia-jia Li; Bo Meng; Jun Li; Ren-shan Ge

doi:10.1007/s11655-013-1549-9

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Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats

OriginalPaper | Updated：2021-08-27

- Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats
- Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats
- 中国结合医学杂志（英文版） 2014年20卷第11期页码：850-856
- Affiliations：
  
  Department of Anesthesiology, The Second Affiliated Hospital of Wenzhou Medical College,Zhejiang Province,Wenzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81073125), Natural Science Foundation of Zhejiang Province (No. Y2090252), International Cooperation Project of Wenzhou Science and Technology Bureau (No. H20070035)
- DOI：10.1007/s11655-013-1549-9
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-1-28，
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Cao, H., Zheng, Jw., Li, Jj. et al. Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats., Chin. J. Integr. Med. 20, 850–856 (2014). https://doi.org/10.1007/s11655-013-1549-9

Hong Cao, Jin-wei Zheng, Jia-jia Li, et al. Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats[J]. Chinese Journal of Integrative Medicine, 2014,20(11):850-856.
Cao, H., Zheng, Jw., Li, Jj. et al. Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats., Chin. J. Integr. Med. 20, 850–856 (2014). https://doi.org/10.1007/s11655-013-1549-9 DOI：

Hong Cao, Jin-wei Zheng, Jia-jia Li, et al. Effects of curcumin on pain threshold and on the expression of nuclear factor κ B and CX3C receptor 1 after sciatic nerve chronic constrictive injury in rats[J]. Chinese Journal of Integrative Medicine, 2014,20(11):850-856. DOI： 10.1007/s11655-013-1549-9.

摘要

To investigate the effects of curcumin on pain threshold and the expressions of nuclear factor κ B (NF-κ B) and CX3C chemokine receptor 1 (CX3CR1) in spinal cord and dorsal root ganglion (DRG) of the rats with sciatic nerve chronic constrictive injury. One hundred and twenty male Sprague Dawley rats

weighing 220–250 g

were randomly divided into 4 groups. Sham surgery (sham) group: the sciatic nerves of rats were only made apart but not ligated; chronic constrictive injury (CCI) group: the sciatic nerves of rats were only ligated without any drug treatment; curcumin treated injury (Cur) model group: the rats were administrated with curcumin 100 mg/(kg·d) by intraperitoneal injection for 14 days after CCI; solvent control (SC) group: the rats were administrated with the solvent at the same dose for 14 days after CCI. Thermal withdrawal latency (TWL) and mechanical withdrawal threshold (MWT) of rats were respectively measured on pre-operative day 2 and postoperative day 1

10 and 14. The lumbar segment L4–5 of the spinal cord and the L4

L5 DRG was removed at post-operative day 3

7 and 14. The change of nuclear factor κ B (NF-κ B) p65 expression was detected by Western blotting while the expression of CX3CR1 was determined by immunohistochemical staining. Compared with the sham group

the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day (P<0.01)

which reached a nadir on the 3rd day after CCI

and the expressions of NF-κ B p65 and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG. In the Cur group

the TWL of rats were significantly increased than those in the CCI group on post-operative day 7

10 and 14 (P<0.05) and MWT increased than those in the CCI group on post-operative day 10 and 14 (P<0.05). In addition

the administration of curcumin significantly decreased the positive expressions of NF-κ B p65 and CX3CR1 in spinal cord and DRG (P<0.05). Our study suggests that curcumin could ameliorate the CCI-induced neuropathic pain

probably through inhibiting CX3CR1 expression by the activation of NF-κ B p65 in spinal cord and DRG.

Abstract

weighing 220–250 g

10 and 14. The lumbar segment L4–5 of the spinal cord and the L4

L5 DRG was removed at post-operative day 3

the TWL and MWT of rats in the CCI group were significantly decreased on each post-operative day (P<0.01)

which reached a nadir on the 3rd day after CCI

and the expressions of NF-κ B p65 and CX3CR1 were markedly increased in spinal cord dorsal horn and DRG. In the Cur group

the TWL of rats were significantly increased than those in the CCI group on post-operative day 7

10 and 14 (P<0.05) and MWT increased than those in the CCI group on post-operative day 10 and 14 (P<0.05). In addition

probably through inhibiting CX3CR1 expression by the activation of NF-κ B p65 in spinal cord and DRG.

关键词

Curcuminneuropathic painmicroglianuclear factor κ BCX3C chemokine receptor 1

Keywords

Curcuminneuropathic painmicroglianuclear factor κ BCX3C chemokine receptor 1

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