Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway

Jin-yan Zhao; Zhi-hong Chen; Wei Lin; Xiao-yong Zhong; Xu-zheng Chen; Jun Peng; Zhen-feng Hong

doi:10.1007/s11655-013-1581-9

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Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway

OriginalPaper | Updated：2021-08-27

- Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway
- Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway
- 中国结合医学杂志（英文版） 2014年20卷第2期页码：123-129
- Affiliations：
  
  1. Fujian Academy of Integrative Medicine,Fuzhou,China
  2. Fujian Key Laboratory of Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese Medicine,Fujian,Fuzhou,China
  3. Fujian Guizhentang Pharmaceutical Co., Ltd.,Fujian Province,Quanzhou,China
- Author bio：
- Funds：
  
  Supported by Important Science & Technology Specific Projects of Fujian Province (No.2010YZ0001-1 and 2010Y2004);Developmental Fund of Chen Keji Integrative Medicine (No. CKJ 2010019)
- DOI：10.1007/s11655-013-1581-9
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-3-6，
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Zhao, Jy., Chen, Zh., Lin, W. et al. Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway., Chin. J. Integr. Med. 20, 123–129 (2014). https://doi.org/10.1007/s11655-013-1581-9

Jin-yan Zhao, Zhi-hong Chen, Wei Lin, et al. Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(2):123-129.
Zhao, Jy., Chen, Zh., Lin, W. et al. Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway., Chin. J. Integr. Med. 20, 123–129 (2014). https://doi.org/10.1007/s11655-013-1581-9 DOI：

Jin-yan Zhao, Zhi-hong Chen, Wei Lin, et al. Bear bile powder (熊胆粉) induces apoptosis of human hepatocellular carcinoma cells via mitochondrion-dependent pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(2):123-129. DOI： 10.1007/s11655-013-1581-9.

摘要

To evaluate the effect of Bear Bile Powder(熊胆粉

BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells

and investigate the possible molecular mechanisms mediating its anti-cancer activity. HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24

48 and 72 h. The viability of HePG2 cells was determined by MTT assay. Cellular morphology was observed via phase-contrast microscopy. Fluorescence-activated cell sorting analysis with Annexin-V/propidium idodide and 5

5′

6′-tetrachloro-1

1′

3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential

respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay. The treatment with 0.4–1 mg/mL of BBP for 24

or 72 h respectively reduced cell viability significantly by 7%–60%

20%–90% or 25%–98%

compared with the untreated control cells (P<0.01). In addition

BBP treatment induced morphological changes in HepG2 cells. Furthermore

after treated with 0

0.4

0.6

0.8 and 1.0 mg/mL of BBP

apoptosis cells (including early and late apoptotic cells) were 18.0%±1.3%

34.9%±2.2%

33.9%±2.8%

37.4%±2.8% and 46.0%±2.5%

respectively (P<0.05); and the percentage of cells with reduced JC-1 red fluorescence were 6.6%±0.8%

8.5%±0.8%

13.5%±1.6%

17.6%±2.3% and 46.7%±3.6%

respectively (P<0.01). Finally

BBP treatment significantly and dose-dependently induced activation of both caspase-9 and caspase-3 in HepG2 cells (P<0.05). BBP could inhibit the growth of HepG2 hepatocellular cancer cells through mitochondrion-mediated apoptosis

which may

in part

explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.

Abstract

To evaluate the effect of Bear Bile Powder(熊胆粉

BBP) on the growth and apoptosis of HepG2 human hepatocellular carcinoma cells

and investigate the possible molecular mechanisms mediating its anti-cancer activity. HepG2 cells were treated with 0.4–1.0 mg/mL of BBP for 24

5′

6′-tetrachloro-1

1′

3′-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) staining was performed to determine cell apoptosis and the loss of mitochondrial membrane potential

respectively. Activation of caspase-9 and -3 was evaluated by a colorimetric assay. The treatment with 0.4–1 mg/mL of BBP for 24

or 72 h respectively reduced cell viability significantly by 7%–60%

20%–90% or 25%–98%

compared with the untreated control cells (P<0.01). In addition

BBP treatment induced morphological changes in HepG2 cells. Furthermore

after treated with 0

0.4

0.6

0.8 and 1.0 mg/mL of BBP

apoptosis cells (including early and late apoptotic cells) were 18.0%±1.3%

34.9%±2.2%

33.9%±2.8%

37.4%±2.8% and 46.0%±2.5%

respectively (P<0.05); and the percentage of cells with reduced JC-1 red fluorescence were 6.6%±0.8%

8.5%±0.8%

13.5%±1.6%

17.6%±2.3% and 46.7%±3.6%

respectively (P<0.01). Finally

which may

in part

explain its anti-cancer activity. BBP may be a potential novel therapeutic agent for the treatment of hepatocellular carcinoma.

关键词

bear bile powderhepatocellular carcinomaapoptosismitochondria

Keywords

bear bile powderhepatocellular carcinomaapoptosismitochondria

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