Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion

Xiao-bo Chang; Shu Wang; Zhi-hong Meng; Xiao-nong Fan; Xue Yang; Xue-min Shi

doi:10.1007/s11655-013-1584-6

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Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion

OriginalPaper | Updated：2021-08-27

- Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion
- Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion
- 中国结合医学杂志（英文版） 2014年20卷第2期页码：130-135
- Affiliations：
  
  1. First Hospital Affiliated to Tianjin University of Traditional Chinese Medicine,Tianjin,China
  2. Moxibustion College of Jiangxi University of Traditional Chinese Medicine,Nanchang,China
  3. Critical Research Room of Encephalopathy Acupuncture Therapy,Tianjin,China
  4. Class II Laboratory of Acupuncture Quantity-Effect Relationship Affiliated to the State, Administration of Traditional Chinese Medicine,Tianjin,China
  5. Mathematics College of Tianjin Polytechnic University,Tianjin,China
  6. Tianjin Key Laboratory of Acupuncture and Moxibustion Science,Tianjin,China
- Author bio：
- Funds：
  
  Supported by the Development Plan of National Critical and Basic Research (No. 2012CB518505, 2010CB530500, and 2006CB504504)
- DOI：10.1007/s11655-013-1584-6
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-3-6，
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Chang, Xb., Wang, S., Meng, Zh. et al. Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion., Chin. J. Integr. Med. 20, 130–135 (2014). https://doi.org/10.1007/s11655-013-1584-6

Xiao-bo Chang, Shu Wang, Zhi-hong Meng, et al. Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion[J]. Chinese Journal of Integrative Medicine, 2014,20(2):130-135.
Chang, Xb., Wang, S., Meng, Zh. et al. Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion., Chin. J. Integr. Med. 20, 130–135 (2014). https://doi.org/10.1007/s11655-013-1584-6 DOI：

Xiao-bo Chang, Shu Wang, Zhi-hong Meng, et al. Study on acupuncture parameters impacting on the acupuncture effect using cluster analysis in a rat model with middle cerebral artery occlusion[J]. Chinese Journal of Integrative Medicine, 2014,20(2):130-135. DOI： 10.1007/s11655-013-1584-6.

摘要

To investigate the influence of acupuncture parameters on the acupuncture effect through the cluster analysis in Wistar rat model with middle cerebral artery occlusion (MCAO). Duplicated MCAO models by Zea-longa’s thread ligation and chose rats with 1–3 scores assessed by Zausinger’s six-score method to be grouped. The rats were divided into 6 basic control groups [(including a normal group

a sham group

a model control group

a model group without intervention

a Nimodipine group

a lateral-to-Renzhong (DU6) group] and 6 acupuncture groups [a Neiguan (PC6) group

a Weizhong (BL40) group

a Sanyinjiao (SP6) group

a Chize (LU5) group

a Renzhong (DU6) group and a Feixue (non-acupoint) group]. In the acupuncture groups

for every acupoint or needling site

9 different parameters [2 factors (frequency and time) and 3 levels (180

120

and 60 cpm of the frequency and 5

and 180 s of the time)] were set respectively by the orthogonal intersection method

in total 54 groups. The rats were treated by acupuncture with a lifting-thrusting manipulation once every 12 h

in total 6 times. Neurobehavioral scores

cerebral blood flow

infarction rate

microcirculation

light microscopy

etc. were measured. The factor analysis was first applied to get the comprehensive effect scores of the samples in the acupuncture groups and then by which the cluster analysis was made with the statistical software of SPSS17.0. For the Neiguan (PC6) group

the exceptional results of acupuncture comprehensive effect were parameters 7

10; the valid results were parameters 2

and the invalid were parameters 5

6. For the Weizhong (BL40) group

the exceptional results were parameters 2

4; the valid results were parameters 3

and the invalid were parameters 8

10. For the Chize (LU5) group

the exceptional results were parameters 7

8; the valid results were parameters 3

10; and the invalid was parameter 2. For the Sanyinjiao (SP6) group

the exceptional results were parameters 4

6; the valid results were parameters 2

5; and the invalid were parameters 7

10. For the Renzhong (DU6) group

the exceptional results were parameters 3

10; the valid results were parameters 2

5; and the invalid was parameter 8. For the Non-acupoint group

the exceptional result was parameter 10; the valid results were parameters 2

9; and the invalid were parameters 5

8. For each meridian acupoint

different acupuncture parameters could consequently get a different acupuncture effect; each meridian acupoint had the most suitable or optimal acupuncture parameters; acupuncture parameters might be the main factors impacting on acupuncture effect.

Abstract

a sham group

a model control group

a model group without intervention

a Nimodipine group

a lateral-to-Renzhong (DU6) group] and 6 acupuncture groups [a Neiguan (PC6) group

a Weizhong (BL40) group

a Sanyinjiao (SP6) group

a Chize (LU5) group

a Renzhong (DU6) group and a Feixue (non-acupoint) group]. In the acupuncture groups

for every acupoint or needling site

9 different parameters [2 factors (frequency and time) and 3 levels (180

120

and 60 cpm of the frequency and 5

and 180 s of the time)] were set respectively by the orthogonal intersection method

in total 54 groups. The rats were treated by acupuncture with a lifting-thrusting manipulation once every 12 h

in total 6 times. Neurobehavioral scores

cerebral blood flow

infarction rate

microcirculation

light microscopy

the exceptional results of acupuncture comprehensive effect were parameters 7

10; the valid results were parameters 2

and the invalid were parameters 5

6. For the Weizhong (BL40) group

the exceptional results were parameters 2

4; the valid results were parameters 3

and the invalid were parameters 8

10. For the Chize (LU5) group

the exceptional results were parameters 7

8; the valid results were parameters 3

10; and the invalid was parameter 2. For the Sanyinjiao (SP6) group

the exceptional results were parameters 4

6; the valid results were parameters 2

5; and the invalid were parameters 7

10. For the Renzhong (DU6) group

the exceptional results were parameters 3

10; the valid results were parameters 2

5; and the invalid was parameter 8. For the Non-acupoint group

the exceptional result was parameter 10; the valid results were parameters 2

9; and the invalid were parameters 5

8. For each meridian acupoint

关键词

middle cerebral artery occlusionacupuncture parametersacupuncture effectcluster analysisrat

Keywords

middle cerebral artery occlusionacupuncture parametersacupuncture effectcluster analysisrat

references

Chang XB, Wang S, Fan XN, Yan X, Shi XM. Establishment of mathematical model in comprehensive evaluating effect of acupuncture in rats with cerebral occlusion. Tianjin J Tradit Chin Med (Chin) 2012;29:65–68.

Longa EZ, Weinstein PR, Carlson S. Reversible middle cerebral artery occlusion without craniectomy in rats. Stroke 1989;20:84–91.

Zausinger S, Hungerhuber E, Baethmann A, Reulen H, Schmid-Elsaesser R. Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms. Brain Res 2000;863:94–105.

Li ZR, ed. Experimental acupuncture science. Beijing: China Press of Traditional Chinese Medicine; 2003:327.

Bao XM, Shu SY, eds. The rat brain in stereotaxic coordinates. Beijing: People’s Medical Publishing House;1991:21–29.

Bian JL, Zhang CH, Shi XM. Conception and core of Academician Shi Xue-min’s acupuncture manipulation quantitative arts. Chin Acupunct Moxibust (Chin) 2003;23:287–289.

Shi XM, Zhang CS, Liu BX, Zhou JZ, Wang CX, Han JX, et al. Manipulation application of reinforcing-reduction by twisting and twirling and quantity conception. China J Tradit Chin Med Pharm (Chin) 1987;4(5):16.

Wang P, Yang HY, Hu YE. Study on the quantification project of electro-acupuncture stimulation quantity. Chin Acupunct Moxibust (Chin) 2009;29:417–420.

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Zhang WT, ed. Advanced tutorials of SPSS statistical analysis. Beijing: Higher Education Press; 2004:218.

Wei YY, Fan XN, Wang S, Shi XM. Status and progress of stimulating parameters in acupuncture treatment of ischemic cerebro-vascular disease. Acupunct Res (Chin) 2008;4:287–288.

Chang XB, Fan XN, Wang S, Meng ZH, Yan X, Shi XM. Cluster analysis for acupoint specificity of acupuncture effect based on cerebral infarction rat model. Chin J Integr Med 2013;19:853–858.

Chang XB, Fan XN, Wang S, Shi XM. Research on the origin of “acupuncture quantity”. J Tradit Chin Med (Chin) 2011;62:363–366.

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