Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats

Kun Zhang; Miao-na Jiang; Cai-hua Zhang; Cong Li; Yu-jie Jia

doi:10.1007/s11655-013-1597-1

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Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats

OriginalPaper | Updated：2021-08-27

- Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats
- Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats
- 中国结合医学杂志（英文版） 2014年20卷第6期页码：438-444
- Affiliations：
  
  1. Department of Pathophysiology, Dalian Medical University,Liaoning Province,Dalian,China
  2. Department of Pathophysiology, Baotou Medical College,Inner Mongolia,Baotou,China
- Author bio：
- Funds：
  
  Supported by Natural Science Fund of Liaoning Province, China (No. 201102055)
- DOI：10.1007/s11655-013-1597-1
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2013-8-29，
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Zhang, K., Jiang, Mn., Zhang, Ch. et al. Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats., Chin. J. Integr. Med. 20, 438–444 (2014). https://doi.org/10.1007/s11655-013-1597-1

Kun Zhang, Miao-na Jiang, Cai-hua Zhang, et al. Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(6):438-444.
Zhang, K., Jiang, Mn., Zhang, Ch. et al. Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats., Chin. J. Integr. Med. 20, 438–444 (2014). https://doi.org/10.1007/s11655-013-1597-1 DOI：

Kun Zhang, Miao-na Jiang, Cai-hua Zhang, et al. Effects of Ganfukang (肝复康) on expression of connective tissue growth factor and focal adhesion kinase/protein kinase B signal pathway in hepatic fibrosis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(6):438-444. DOI： 10.1007/s11655-013-1597-1.

摘要

To investigate the effect of Ganfukang (肝复康

GFK) on connective tissue growth factor (CTGF) and focal adhesion kinase (FAK)/protein kinase B (PKB or Akt) signal pathway in a hepatic fibrosis rat model and to explore the underlying therapeutic molecular mechanisms of GFK. Fifty SD rats were randomly divided into five groups as follows: the control group

the model group (repeated subcutaneous injection of CCl4)

and the three GFK treatment groups (31.25

312.5

and 3125 mg/kg

intragastric administration). Reverse transcriptase-polymerase chain reaction (RT-PCR)

Western blotting

and immunohistochemistry were used to examine the expression of CTGF

integrin α5

integrin β1

FAK/Akt signal pathway

cyclinD1

and collagen in the different-treated rats. GFK attenuated the up-regulation of CTGF

integrin α5

and integrin β1 in hepatic fibrosis rats and suppressed both the phosphorylation of FAK and the phosphorylation of Akt simultaneously (P<0.01). At the same time

the expression of cyclinD1

collagen I

and collagen III was decreased by GFK significantly (P<0.01). CTGF and FAK/Akt signal pathway were activated in the CCl4-induced hepatic fibrosis rats

which contribute to increased expression of cyclinD1 and collagen genes. The mechanisms of the anti-fibrosis activity of GFK may be due to its effects against CTGF and FAk/Akt signal pathway.

Abstract

To investigate the effect of Ganfukang (肝复康

the model group (repeated subcutaneous injection of CCl4)

and the three GFK treatment groups (31.25

312.5

and 3125 mg/kg

intragastric administration). Reverse transcriptase-polymerase chain reaction (RT-PCR)

Western blotting

and immunohistochemistry were used to examine the expression of CTGF

integrin α5

integrin β1

FAK/Akt signal pathway

cyclinD1

and collagen in the different-treated rats. GFK attenuated the up-regulation of CTGF

integrin α5

and integrin β1 in hepatic fibrosis rats and suppressed both the phosphorylation of FAK and the phosphorylation of Akt simultaneously (P<0.01). At the same time

the expression of cyclinD1

collagen I

and collagen III was decreased by GFK significantly (P<0.01). CTGF and FAK/Akt signal pathway were activated in the CCl4-induced hepatic fibrosis rats

which contribute to increased expression of cyclinD1 and collagen genes. The mechanisms of the anti-fibrosis activity of GFK may be due to its effects against CTGF and FAk/Akt signal pathway.

关键词

connective tissue growth factorGanfukangHepatic FibrosisFAK/Akt signal pathway

Keywords

connective tissue growth factorGanfukangHepatic FibrosisFAK/Akt signal pathway

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