Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia

Di-jiong Wu; Bao-dong Ye; Zhi-ping Hu; Yi-ping Shen; Jian-ping Shen; Sheng-yun Lin; Ming-tao Chen; Yong-lin Liu; Yu-hong Zhou

doi:10.1007/s11655-013-1652-y

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Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia

OriginalPaper | Updated：2021-08-27

- Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia
- Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia
- 中国结合医学杂志（英文版） 2013年19卷第12期页码：905-912
- Affiliations：
  
  Department of Hematology, the First Affi liated Hospital, Zhejiang University of Traditional Chinese Medicine,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by Special Item of Important Disease of Zhejiang Province Traditional Chinese Medicine Sic-Tech Innovation Platform (No. 2009ZDJB01), 2011 Special Research Funds for Traditional Chinese Medicine Industry (No. 201107001), Subject of Key Sic-Tech Innovation Team of Zhejiang Province (No. 2011R09042-02), Program of Science and Technology Department of Zhejiang Province (No. 2006C23039) and Zhejiang Provincial Traditional Chinese Medicine Administration Bureau Program (No. 2007GA004)
- DOI：10.1007/s11655-013-1652-y
  中图分类号：
- 纸质出版日期：2013，
  
  网络出版日期：2013-12-5，
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Wu, Dj., Ye, Bd., Hu, Zp. et al. Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia., Chin. J. Integr. Med. 19, 905–912 (2013). https://doi.org/10.1007/s11655-013-1652-y

Di-jiong Wu, Bao-dong Ye, Zhi-ping Hu, et al. Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia[J]. Chinese Journal of Integrative Medicine, 2013,19(12):905-912.
Wu, Dj., Ye, Bd., Hu, Zp. et al. Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia., Chin. J. Integr. Med. 19, 905–912 (2013). https://doi.org/10.1007/s11655-013-1652-y DOI：

Di-jiong Wu, Bao-dong Ye, Zhi-ping Hu, et al. Bone marrow angiogenesis in patients presenting with differential Chinese medicine syndrome: Correlation with the clinico-pathological features of aplastic anemia[J]. Chinese Journal of Integrative Medicine, 2013,19(12):905-912. DOI： 10.1007/s11655-013-1652-y.

摘要

To explore differences in bone marrow angiogenesis seen in aplastic anemia (AA) patients presenting with differential Chinese medicine (CM) syndrome

and to correlate these differences with clinical pathology. Thirty-five patients were enrolled

including 18 with “yang deficiency syndrome” and 17 with “yin deficiency syndrome.” Bone marrow biopsies and serum were collected. Microvessel density (MVD) and positive expression of vascular endothelial-derived growth factor (VEGF) were detected by immunohistochemisty. Hypoxia inducible factor −1α (HIF-1α)

and VEGF expression were assayed by enzyme-linked immunoabsorbent assay (ELISA)

serum lactate dehydrogenase (LDH) was tested by enzyme method and liquid chip technology was used to detected the expression of interleukin (IL)-2

IL-4

IL-6

IL-10

interferon (IFN)-γ and tumor necrosis factor (TNF)-α. Counts for leukocytes

absolute neutrophils and platelets in “yin deficiency syndrome” were lower than those found in “yang deficiency syndrome” (P<0.05). MVD and VEGF expression

and the positive rate of CD34 and VEGF in bone marrow were lower in AA

especially in “yin deficiency syndrome” (P<0.01 or P<0.05). “Yin deficiency syndrome” displayed decreased VEGF and LDH expression

and enhanced expression of HIF-1α as compared to “yang deficiency syndrome” (P<0.05). Levels of IL-4 and IL-6 were higher in AA (P<0.01)

but IL-10 was decreased (P<0.05). High TNF-α expression was seen in “yang deficiency syndrome” and IFN-γ expression was decreased in “yin deficiency syndrome” as compared with normals (P <0.01 and P<0.05

respectively). AA patients have lower MVD than normals

especially in “yin deficiency syndrome.” MVD might differentially correlate to disease severity

and could be dependent on bone marrow or serum VEGF expression and LDH. Additionally

IL-2

IL-10

IL-4 and IFN-γ were negatively associated while IL-6 and TNF-α were positively associated with MVD.

Abstract

To explore differences in bone marrow angiogenesis seen in aplastic anemia (AA) patients presenting with differential Chinese medicine (CM) syndrome

and to correlate these differences with clinical pathology. Thirty-five patients were enrolled

and VEGF expression were assayed by enzyme-linked immunoabsorbent assay (ELISA)

serum lactate dehydrogenase (LDH) was tested by enzyme method and liquid chip technology was used to detected the expression of interleukin (IL)-2

IL-4

IL-6

IL-10

interferon (IFN)-γ and tumor necrosis factor (TNF)-α. Counts for leukocytes

absolute neutrophils and platelets in “yin deficiency syndrome” were lower than those found in “yang deficiency syndrome” (P<0.05). MVD and VEGF expression

and the positive rate of CD34 and VEGF in bone marrow were lower in AA

especially in “yin deficiency syndrome” (P<0.01 or P<0.05). “Yin deficiency syndrome” displayed decreased VEGF and LDH expression

and enhanced expression of HIF-1α as compared to “yang deficiency syndrome” (P<0.05). Levels of IL-4 and IL-6 were higher in AA (P<0.01)

respectively). AA patients have lower MVD than normals

especially in “yin deficiency syndrome.” MVD might differentially correlate to disease severity

and could be dependent on bone marrow or serum VEGF expression and LDH. Additionally

IL-2

IL-10

IL-4 and IFN-γ were negatively associated while IL-6 and TNF-α were positively associated with MVD.

关键词

aplastic anemiaangiogenesisChinese medicine syndromeclinico-pathologicalvascular endothelial-derived growth factortumor necrosis factor-α

Keywords

aplastic anemiaangiogenesisChinese medicine syndromeclinico-pathologicalvascular endothelial-derived growth factortumor necrosis factor-α

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