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Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)
Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)
- 中国结合医学杂志（英文版） 2013年19卷第12期 页码：918-926
- Affiliations：
1. Institute of Nephrology, State Key Laboratory of Kidney Disease (2011DAV)00088, Chinese PLA General Hospital,Beijing,China
2. Department of Nephrology, 2nd Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：Supported by the National Basic Research Program of China (No. 2011CB944004), the Program of the National Natural Science Foundation of China (No. 30971377) and the Science and Technology Project of Beijing, China (No. D09050704310904);These authors contributed equally to this work
DOI：10.1007/s11655-013-1654-9
中图分类号：纸质出版日期：2013，
网络出版日期：2013-12-5，
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Lu, Zy., Liu, Sw., Xie, Ys. et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)., Chin. J. Integr. Med. 19, 918–926 (2013). https://doi.org/10.1007/s11655-013-1654-9
Zhao-yu Lu, Shu-wen Liu, Yuan-sheng Xie, et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)[J]. Chinese Journal of Integrative Medicine, 2013,19(12):918-926.
Lu, Zy., Liu, Sw., Xie, Ys. et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)., Chin. J. Integr. Med. 19, 918–926 (2013). https://doi.org/10.1007/s11655-013-1654-9 DOI：
Zhao-yu Lu, Shu-wen Liu, Yuan-sheng Xie, et al. Inhibition of the tubular epithelial-to-mesenchymal transition in vivo and in vitro by the Uremic Clearance Granule (尿毒清颗粒)[J]. Chinese Journal of Integrative Medicine, 2013,19(12):918-926. DOI： 10.1007/s11655-013-1654-9.
摘要
To investigate the effect of the Uremic Clearance Granule (UCG
尿毒清颗粒)
a Chinese patent medicine
on tubular epithelial-to-mesenchymal transition (EMT) in a unilateral ureteral obstruction (UUO) model in vivo and transforming growth factor (TGF)-β1 induced EMT of HK-2 cells in vitro. In vivo study
50 Sprague Dawley rats were divided into three groups: a sham operation group (n=10)
a UUO group (n=20)
and a UUO with UCG treatment group (n=20). The UCG was given at a dose of 4.5 g/kg body weight per day by gavage after surgery. In vitro study
HK-2 cells were cultured in 10% fetal bovine serum (FBS)
10% healthy rat serum
10% FBS and TGF-β1 (10 ng/mL)
10% healthy rat serum and TGF-β1
or 10% rat serum containing the uremic clearance granule and TGF-β1. The expression of the epithelial marker E-cadherin and the mesenchymal markers vimentin and α-smooth muscle actin (α-SMA) in kidney tissues and HK-2 cells were investigated by Western blot analysis and immunofluorescence staining. The rats of the UUO group showed obvious tubulointerstitial fibrosis
compared with the sham operation group rats. Tubulointerstitial fibrosis score was reduced by 17.5%±1.1% at day 7 and by 20.0%±1.2% at day 14 in the UCG-treated group
compared with the UUO group. The UCG could maintained expression of E-cadherin and suppressed expression of vimentin and α-SMA in kidney tissues of UUO rats at days 7 and 14
as determined by Western blot analysis and immunofluorescence staining. Rat serum containing the UCG partially inhibited TGF-β1-induced fibroblast phenotype of HK-2 cells and maintained the epithelial morphology of HK-2 cells in vitro. This occurred partially through a reduction of vimentin expression and an increase of E-cadherin expression. These results suggest that the UCG prevents tubular EMT and may be a promising agent for treating tubulointerstitial fibrosis.
Abstract
To investigate the effect of the Uremic Clearance Granule (UCG
尿毒清颗粒)
a Chinese patent medicine
on tubular epithelial-to-mesenchymal transition (EMT) in a unilateral ureteral obstruction (UUO) model in vivo and transforming growth factor (TGF)-β1 induced EMT of HK-2 cells in vitro. In vivo study
50 Sprague Dawley rats were divided into three groups: a sham operation group (n=10)
a UUO group (n=20)
and a UUO with UCG treatment group (n=20). The UCG was given at a dose of 4.5 g/kg body weight per day by gavage after surgery. In vitro study
HK-2 cells were cultured in 10% fetal bovine serum (FBS)
10% healthy rat serum
10% FBS and TGF-β1 (10 ng/mL)
10% healthy rat serum and TGF-β1
or 10% rat serum containing the uremic clearance granule and TGF-β1. The expression of the epithelial marker E-cadherin and the mesenchymal markers vimentin and α-smooth muscle actin (α-SMA) in kidney tissues and HK-2 cells were investigated by Western blot analysis and immunofluorescence staining. The rats of the UUO group showed obvious tubulointerstitial fibrosis
compared with the sham operation group rats. Tubulointerstitial fibrosis score was reduced by 17.5%±1.1% at day 7 and by 20.0%±1.2% at day 14 in the UCG-treated group
compared with the UUO group. The UCG could maintained expression of E-cadherin and suppressed expression of vimentin and α-SMA in kidney tissues of UUO rats at days 7 and 14
as determined by Western blot analysis and immunofluorescence staining. Rat serum containing the UCG partially inhibited TGF-β1-induced fibroblast phenotype of HK-2 cells and maintained the epithelial morphology of HK-2 cells in vitro. This occurred partially through a reduction of vimentin expression and an increase of E-cadherin expression. These results suggest that the UCG prevents tubular EMT and may be a promising agent for treating tubulointerstitial fibrosis.
关键词
Uremic Clearance Granuleepithelial-to-mesenchymal transitiontubulointerstitial fibrosis
Keywords
Uremic Clearance Granuleepithelial-to-mesenchymal transitiontubulointerstitial fibrosis
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