Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)

Ping Xue; Jia Guo; Xiao-nan Yang; Wei Huang; Qing Xia

doi:10.1007/s11655-013-1661-x

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Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)

OriginalPaper | Updated：2021-08-27

- Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)
- Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)
- 中国结合医学杂志（英文版） 2014年20卷第10期页码：770-775
- Affiliations：
  
  1. Pancreatic Diseases Research Group, Department of Integrated Traditional and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
  2. Liverpool NIHR Pancreas Biomedical Research Unit, Royal Liverpool University Hospital, University of Liverpool,Liverpool,UK
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81072910) and Science and Technology Support Program of Sichuan (No. 2011SZ0291, 2010SZ0068)
- DOI：10.1007/s11655-013-1661-x
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-1-23，
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Xue, P., Guo, J., Yang, Xn. et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)., Chin. J. Integr. Med. 20, 770–775 (2014). https://doi.org/10.1007/s11655-013-1661-x

Ping Xue, Jia Guo, Xiao-nan Yang, et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)[J]. Chinese Journal of Integrative Medicine, 2014,20(10):770-775.
Xue, P., Guo, J., Yang, Xn. et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)., Chin. J. Integr. Med. 20, 770–775 (2014). https://doi.org/10.1007/s11655-013-1661-x DOI：

Ping Xue, Jia Guo, Xiao-nan Yang, et al. Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)[J]. Chinese Journal of Integrative Medicine, 2014,20(10):770-775. DOI： 10.1007/s11655-013-1661-x.

摘要

To investigate effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on changes of neuronal acetylcholine receptor alpha 7 (nAChRα7) of peritoneal macrophages in acute pancreatitis (AP). Eighteen Kunming mice were equally randomized into the control group

AP group and CQCQD treatment group. AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart

while control mice received saline injections. At 72 h after the first injection of L-arginine

mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times

whilst mice in the other two groups received the same amount of saline feeding. Mice were sacrificed by cervical dislocation 2 h after the last feeding of either CQCQD or saline. Peritoneal macrophages were collected for determination of nAChRα7 mRNA and protein expression. Serum was collected for detection of interleukin-6 (IL-6)

IL-10 and acetylcholine (ACh) levels

and pancreas was for histopathology analysis. The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score (4.5±0.5 vs. 6.2±1.7

P<0.05) and the serum IL-6 levels (1228.3±419.2 pg/mL vs. 1589.6±337.3 pg/mL

P<0.05). The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group (P>0.05)

but were significantly up-regulated after the CQCQD treatment (P<0.05). The serum ACh levels in the AP group were significantly lower than those in the control group (3.1±0.6 μg/mL vs 4.8±0.7 μg/mL P<0.05)

but were significantly increased after the CQCQD treatment (5.6±1.5 μg/mL vs 3.1±0.6 μg/mL

P<0.05). CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChRα7 of peritoneal macrophages.

Abstract

To investigate effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on changes of neuronal acetylcholine receptor alpha 7 (nAChRα7) of peritoneal macrophages in acute pancreatitis (AP). Eighteen Kunming mice were equally randomized into the control group

AP group and CQCQD treatment group. AP was induced by two intraperitoneal injections of 4 g/kg L-arginine at 1 h apart

while control mice received saline injections. At 72 h after the first injection of L-arginine

mice in the treatment group were intragastrically administered 0.1 mL/10 g CQCQD every 2 h for 3 times

IL-10 and acetylcholine (ACh) levels

and pancreas was for histopathology analysis. The CQCQD treatment significantly ameliorated the severity of AP as evidenced by reducing the pancreatic histopathology score (4.5±0.5 vs. 6.2±1.7

P<0.05) and the serum IL-6 levels (1228.3±419.2 pg/mL vs. 1589.6±337.3 pg/mL

P<0.05). The mRNA and protein expression of nAChRα7 of the peritoneal macrophages in the AP group were similar to the control group (P>0.05)

but were significantly increased after the CQCQD treatment (5.6±1.5 μg/mL vs 3.1±0.6 μg/mL

P<0.05). CQCQD is protective against L-arginine-induced AP through mechanisms involving nAChRα7 of peritoneal macrophages.

关键词

acetylcholineAcute PancreatitisChaiqin Chengqi Decoctioncholinergic anti-inflammatory pathwayneuronal acetylcholine receptor alpha 7

Keywords

acetylcholineAcute PancreatitisChaiqin Chengqi Decoctioncholinergic anti-inflammatory pathwayneuronal acetylcholine receptor alpha 7

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