Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model

Hu-jun Wang; Wei-cheng Gao; Shao-lin Ma

doi:10.1007/s11655-014-1622-4

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Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model

OriginalPaper | Updated：2021-08-27

- Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model
- Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model
- 中国结合医学杂志（英文版） 2015年21卷第7期页码：537-541
- Affiliations：
  
  Department of Plastic Surgery of the First Teaching Hospital, Xinjiang Medical University,Urumqi,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Xinjiang Uygur Autonomous Region of China (No. 2009211A16), and the National Natural Science Foundation of China (No. 81260291)
- DOI：10.1007/s11655-014-1622-4
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-1-21，
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Wang, Hj., Gao, Wc. & Ma, Sl. Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model., Chin. J. Integr. Med. 21, 537–541 (2015). https://doi.org/10.1007/s11655-014-1622-4

Hu-jun Wang, Wei-cheng Gao, Shao-lin Ma. Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model[J]. Chinese Journal of Integrative Medicine, 2015,21(7):537-541.
Wang, Hj., Gao, Wc. & Ma, Sl. Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model., Chin. J. Integr. Med. 21, 537–541 (2015). https://doi.org/10.1007/s11655-014-1622-4 DOI：

Hu-jun Wang, Wei-cheng Gao, Shao-lin Ma. Effect of Abnormal Savda Munziq on hypertrophic scar formation in a rabbit ear model[J]. Chinese Journal of Integrative Medicine, 2015,21(7):537-541. DOI： 10.1007/s11655-014-1622-4.

摘要

To investigate whether administrating Abnormal Savda Munziq (ASMq)

a traditional Uighur herbal preparation used for the prevention or treatment of diseases

affects hypertrophic scar (HTS) formation by using an established rabbit ear model. The HTS rabbit model was created by circular fullthickness skin excisions on both ears of rabbits. Twenty rabbits were randomized into four groups

with 5 rabbits and 60 wounds in each group. Group A was the control group

treated with normal saline daily. Groups B

and D were the treatment groups at three different doses of ASMq (400

800

and 1200 mg/kg body weight

respectively

daily

by gastrogavage). Twenty wounds were randomly chosen from each group on the 40th day after treatment and specimen were examined. Scar elevation index (SEI) was analyzed with histological assessment

and ultrastructure analysis was analyzed with a transmission electron microscopy. Groups B

and D demonstrated significant reductions in SEI as compared with the control group at 35.9% (P=0.0212)

48.2% (P=0.0108)

and 52.7% (P=0.0103)

respectively in a dose-response manner. SEI was lowered in Group D compared with Group B with a significant difference (P=0.015). However

there were no significant differences between Groups B and C

or between Groups C and D. Histological analysis showed that highdose ASMq (1200 mg/kg) could enhance the softening of HTS of rabbit ears and increase the compliance as shown in general. Ultrastructure analysis showed that with increased ASMq dose

the fibroblasts

pro-collagen

collagen

endoplasmic reticulum and ribosomes were reduced gradually. Orally administered ASMq significantly reduces the severity of HTS in the rabbit ear model. The findings of this study may have clinical implications on the management of human HTS.

Abstract

To investigate whether administrating Abnormal Savda Munziq (ASMq)

a traditional Uighur herbal preparation used for the prevention or treatment of diseases

with 5 rabbits and 60 wounds in each group. Group A was the control group

treated with normal saline daily. Groups B

and D were the treatment groups at three different doses of ASMq (400

800

and 1200 mg/kg body weight

respectively

daily

by gastrogavage). Twenty wounds were randomly chosen from each group on the 40th day after treatment and specimen were examined. Scar elevation index (SEI) was analyzed with histological assessment

and ultrastructure analysis was analyzed with a transmission electron microscopy. Groups B

and D demonstrated significant reductions in SEI as compared with the control group at 35.9% (P=0.0212)

48.2% (P=0.0108)

and 52.7% (P=0.0103)

respectively in a dose-response manner. SEI was lowered in Group D compared with Group B with a significant difference (P=0.015). However

there were no significant differences between Groups B and C

the fibroblasts

pro-collagen

collagen

关键词

hypertrophic scarAbnormal Savda Munziqultrastructure

Keywords

hypertrophic scarAbnormal Savda Munziqultrastructure

references

Widgerow AD, Chait LA, Stals PJ, Stals R, Candy G. Multimodality scar management program. Aesthetic Plast Surg 2009;33:533᾿43.

Qi SH, Xie JL, Pan S, Xu YB, Li TZ, Tang JM, et al. Effects of asiaticoside on the expression of Smad protein by normal skin fibroblasts and hypertrophic scar fibroblasts. Clin Exp Dermatol 2008;33:171᾿75.

Gauglitz GG, Korting HC, Pavicic T, Ruzicka T, Jeschke MG. Hypertrophic scarring and keloids: pathomechanisms and current and emerging treatment strategies. Mol Med 2011;17:113᾿25.

Armour A, Scott PG, Tredget EE. Cellular and molecular pathology of HTS: basis for treatment. Wound Repair Regen 2007;15:S6–S17.

Wang XQ, Liu YK, Qing C, Lu SL. Hyperactivity of fibroblasts and functional regression of endothelial cells contribute to microvessel occlusion in hypertrophic scarring. Microvasc Res 2009; 77:204᾿11.

Ren HT, Hu H, Li Y, Jiang HF, Hu XL, Han CM. Endostatin inhibits hypertrophic scarring in a rabbit ear model. J Zhejiang Univ (Chin) 2013;14:224᾿30.

Tang B, Zhu B, Liang Y, Bi L, Hu Z, Chen B, et al. Asiaticoside suppresses collagen expression and TGFbeta/ Smad signaling through inducing Smad7 and inhibiting TGF-betaRI and TGF-betaRII in keloid fibroblasts. Arch Dermatol Res 2011;303:563᾿72.

Deng C, Zheng J, Wan W, Zhang S, Ding Z, Mao G, et al. Suppression of cell proliferation and collagen production in cultured human hypertrophic scar fibroblasts by Sp1 decoy oligodeoxynucleotide. Mol Med Rep 2013;7:785᾿90.

Yusup A, Upur H, Umar A, Berke B, Yimit D, Lapham JC, et al. Abnormal Savda Munziq, an herbal preparation of traditional Uighur medicine, may prevent 1,2-dimethylhydrazine-induced rat colon carcinogenesis. Evid Based Complement Alternat Med 2011;2011:152015.

Choi EM, Hwang JK. Antiinflammatory, analgesic and antioxidant activities of the fruit of Foeniculum vulgare. Fitoterapia 2004;75:557᾿65.

Yusup A, Upur H, Baudrimont I, Umar A, Kader T, Begaud B, et al. Cytotoxicity of abnormal Savda Munziq aqueous extract in human hepatoma (HepG2) cells. Fundam Clin Pharmacol 2005;19:465᾿72.

Yusup A, Upur H, Umar A, Moore N. Protective effects of Munziq and Mushil of abnormal Savda to mitochondrial oxidative damage. Fundam Clin Pharmacol 2004;18:471᾿76.

Upur H, Yusup A, Baudrimont I, Umar A, Berke B, Yimit D, et al. Inhibition of cell growth and cellular protein, DNA and RNA synthesis in human Hepatoma (HepG2) cells by ethanol extract of abnormal Savda Munziq of traditional Uighur medicine. Evid Based Complement Alternat Med 2011;25:14᾿4.

Ko JH, Kim PS, Zhao Y, Hong SJ, Mustoe TA. HMG-CoA reductase inhibitors (Statins) reduce hypertrophic scar formation in a rabbit ear wounding model. Plast Reconstr Surg 2012;129:252e᾿61e.

Zhu Z, Ding J, Shankowsky HA, Tredget EE. The molecular mechanism of hypertrophic scar. J Cell Commun Signal 2013;7:239᾿52.

Issa MC, Kassuga LE, Chevrand NS, Pires MT. Topical delivery of triamcinolone via skin pretreated with ablative radiofrequency: a new method in hypertrophic scar treatment. Int J Dermatol 2013;52:367᾿70.

Song R, Bian HN, Lai W, Chen HD, Zhao KS. Normal skin and hypertrophic scar fibroblasts differentially regulate collagen and fibronectin expression as well as mitochondrial membrane potential in response to basic fibroblast growth factor. Braz J Med Biol Res 2011;44:402᾿10.

Foley TT, Ehrlich HP. Through gap junction communications, co-cultured mast cells and fibroblasts generate fibroblast activities allied with hypertrophic scarring. Plast Reconstr Surg 2013;131:1036᾿044.

Verrecchia F, Mauviel A. Transforming growth factor-beta and fibrosis. World J Gastroenterol 2007;13:3056᾿062.

Atiyeh BS. Nonsurgical management of hypertrophic scars: evidence-based therapies, standard practices, and emerging methods. Aesthetic Plast Surg 2007;31:468᾿92.

Upur H, Yusup A, Umar A, Moore N. Uighur traditional medicine syndrome of Abnormal Savda in men is associated with oxidative stress, which can be improved by Munziq and Mushil of Abnormal Savda. Therapie 2004;59:483᾿84.

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