Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro

Rana Keyhanmanesh; Mohammad Hossien Boskabady; Mohammad Ali Ebrahimi Saadatloo; Morteza Boskabady

doi:10.1007/s11655-014-1623-3

Your Location：

Home >

Browse articles >

Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro

OriginalPaper | Updated：2021-08-27

- Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro
- Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro
- 中国结合医学杂志（英文版） 2016年22卷第5期页码：377-383
- Affiliations：
  
  1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz,Iran
  2. Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, Tabriz,Iran
  3. Department of Physiology, Tabriz University of Medical Sciences, Tabriz,Iran
  4. Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad,Iran
  5. Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad,Iran
  6. Department of Basic sciences, Islamic Azad University, Tabriz branch, Tabriz,Iran
- Author bio：
- Funds：
- DOI：10.1007/s11655-014-1623-3
  中图分类号：
- 纸质出版日期：2016，
  
  网络出版日期：2014-8-26，
Scan for full text
Keyhanmanesh, R., Boskabady, M.H., Saadatloo, M.A.E. et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro, ., Chin. J. Integr. Med. 22, 377–383 (2016). https://doi.org/10.1007/s11655-014-1623-3

Rana Keyhanmanesh, Mohammad Hossien Boskabady, Mohammad Ali Ebrahimi Saadatloo, et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro[J]. Chinese Journal of Integrative Medicine, 2016,22(5):377-383.
Keyhanmanesh, R., Boskabady, M.H., Saadatloo, M.A.E. et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro, ., Chin. J. Integr. Med. 22, 377–383 (2016). https://doi.org/10.1007/s11655-014-1623-3 DOI：

Rana Keyhanmanesh, Mohammad Hossien Boskabady, Mohammad Ali Ebrahimi Saadatloo, et al. Contribution of water and lipid soluble substances in the relaxant effects of Tymus vulgaris extract on guinea pig tracheal smooth muscle in vitro[J]. Chinese Journal of Integrative Medicine, 2016,22(5):377-383. DOI： 10.1007/s11655-014-1623-3.

摘要

To examine the relaxant effects of hydro-ethanolic

macerated aqueous (MA) and lipidfree macerated aqueous (LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs. The relaxant effects of five cumulative concentrations of each extract (0.4

0.8

1.2

1.6 and 2.0 g/100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline (0.1

0.2

0.4

0.6 and 0.8 mmol/L) on precontracted tracheal smooth muscle of guinea pig with 60 mmol/L KCl (group 1) and 10 µmol/L methacholine (group 2

n=6 for each group). In group 1 all concentrations of theophylline

three higher concentrations of hydro-ethanolic

two concentrations of LFMA and last concentration of MA extracts showed significant relaxant effects compared with that of saline (P<0.05 or P<0.01). Two lower concentrations of LFMA and all concentrations of MA except higher one caused contraction compared with saline (P<0.05 or 0.01). In group 2 experiments

all concentrations of theophylline

hydro-ethanolic

MA and LFMA extracts showed significant relaxant effects compared to that of saline (P<0.05 or P<0.01). In both groups

the relaxant effect of all concentrations of hydro-ethanolic extract were significantly higher than most concentrations of others (P<0.05 or P<0.01). The relaxant effect of different concentrations of three extracts were significantly greater in group 2 compared with group 1 experiments (all P<0.01). There were significantly positive correlations between the relaxant effects and concentrations for theophylline and all extracts in both groups (P<0.05 or P<0.01). Hydro-ethanolic extract has a potent weaker relaxant effect for other extracts from Tymus vulgaris on tracheal chains of guinea pigs.

Abstract

To examine the relaxant effects of hydro-ethanolic

macerated aqueous (MA) and lipidfree macerated aqueous (LFMA) extract of Tymus vulgaris on tracheal chains of guinea pigs. The relaxant effects of five cumulative concentrations of each extract (0.4

0.8

1.2

1.6 and 2.0 g/100 mL) were compared with saline as negative control and five cumulative concentrations of theophylline (0.1

0.2

0.4

0.6 and 0.8 mmol/L) on precontracted tracheal smooth muscle of guinea pig with 60 mmol/L KCl (group 1) and 10 µmol/L methacholine (group 2

n=6 for each group). In group 1 all concentrations of theophylline

three higher concentrations of hydro-ethanolic

all concentrations of theophylline

hydro-ethanolic

MA and LFMA extracts showed significant relaxant effects compared to that of saline (P<0.05 or P<0.01). In both groups

关键词

Tymus vulgarishydro-ethanolic extractmacerated aqueous extractlipid-free macerated aqueous extractrelaxant effectguinea pigtracheal

Keywords

Tymus vulgarishydro-ethanolic extractmacerated aqueous extractlipid-free macerated aqueous extractrelaxant effectguinea pigtracheal

references

Simon JE, Morales MR, Phippen WB, Vieira RF, Hao Z. A source of aroma compounds and a popular culinary and ornamental herb. In: J Janick, ed. Perspectives on new crops and new uses. Alexandria: ASHS Press; 1999:499–505.

Javanmardi J, Khalighi A, Kashi A, Bais HP, Vivanco JM. Chemical characterization of basil (Ocimum basilicum L.) found in local accessions and used in traditional medicines in Iran. J Agri Food Chem 2002;50:5878–5883.

Senatore F. Influence of harvesting time on yield and composition of the essential oil of a thyme (Thymus pulegioides L.) growing wild in Campania (southern Italy). J Agri Food Chem 1996;44:1327–1332.

Baranauskiene R, Venskutonis PR, Viskelis P, Dambrauskiene E. Influence of nitrogen fertilizers on the yield and composition of thyme (Thymus vulgaris). J Agri Food Chem 2003;51:7751–7758.

Al-Said MS. Traditional medicinal plants of Saudi Arabia. Am J Chin Med 1993;21:291–298.

Vollon C, Chaumont JP. Antifungal properties of essential oils and their main components upon Cryptococcus neoformans. Mycopathology 1994;128:151–153.

Perrucci S, Cecchini S, Pretti C, Varriale Cognetti AM, Macchioni G, Flamini G, et al. In vitro antimycotic activity of some natural products against Saprolegnia ferax. Phytother Res 1995;9:147–149.

Pasteur N, Menasherov M, Ravid U, Juven B. Antifungal activity of oregano and thyme essential oils applied as fumigants against fungi attacking stored grain. J Food Protection 1995;58:81–85.

Tantaouielaraki A, Errifi A. Antifungal activity of essential oils when associated with sodium chloride or fatty acids. Grasas y Aceites 1994;45:363–369.

Janssen AM,Scheffer JJC, Baerheim-Svendsen A. Antimicrobial activity of essential oils: a 1976–1986 literature review. Aspects of the test methods. Planta Med 1987;53:395–398.

Juven BJ, Kanner J, Schved F, Weissslowicz H. Factors that interact with the antibacterial action of thyme essential oil and its active constituents. J Appl Bacteriol 1994;76:626–631.

Czygan C-F. Thymian, Thymi Herba. In: Wichtl M. ed. Teedrogen. 2nd ed. Stuttgart, Wissenschaftliche Verlagsgesellschaft 1989;498–500.

Meister A, Bernhardt G, Christoffel V, Buschauer A. Antispasmodic activity of thymus vulgaris extract on the isolated guinea-pig trachea: discrimination between drug and ethanol effects. Planta Med 1999;65:512–516.

Boskabady MH, Aslani MR, Kiani S. Relaxant effects of Tymus volgari on guinea pig tracheal chains and its possible mechanism(s). Phytother Res 2006;20:28–33.

Babaei M, Abarghoei ME, Ansari R, Vafaei AA, Taherian AA, Akhavan MM et al. Antispasmodic effect of hydroalcoholic extract of Thymus vulgaris on the guinea-pig ileum. Nat Prod Res 2008;22:1143–1150.

Keyhanmanesh R, Boskabady MH. Relaxant effects of different fractions from Tymus vulgaris on guinea-pig tracheal chains and its possible mechanism(s). Biol Res 2012;45:67–73.

Reiter M, Brandt W. Relaxant effects on tracheal and ileal smooth muscles of the guinea pig. Arzneim Forsch Drug Res 1985;35:408–414.

Holroyde MC. The influence of epithelium on the responsiveness of guinea-pig isolated trachea. Br J Pharmacol 1986;87:501–507.

Boskabady MH, Jandaghi P. Relaxant effect of carvacrol on guinea pig tracheal chains and its possible mechanisms. Pharmazie 2003;58:661–663.

Martin CAE, Naline E, Bakdach H, Advenier C. Beta3 adrenoceptor agonists, BRL 37344 and SR58611 A do not induce relaxation of human, sheep and guinea-pig airway smooth muscle in vitro. Eur Respir J 1994;7:1610–1615.

Boskabady MH, Khatami A, Nazari A. Possible mechanism(s) for relaxant effects of Foeniculum vulgare on guinea pig tracheal chains. Pharmazie 2004;59:561–564.

Rabe KF, Magnussen H, Dent G. Theophylline and selective PDE inhibitors as bronchodilators and smooth muscle relaxants. Eur Respir J 1995;8:637–642.

Daly JW, Jacobson KA, Ukena D. Adenosine receptors: development of selective agonists and antagonists. Prog Clin Biol Res 1987;230:41–63.

Van Den Broucke CO. Chemical and pharmacological investigation on Thymi herba and its liquid extracts. Planta Med 1980;39:253–254.

Van Den Broucke CO, Lemli JA. Pharmalogical and chemical investigation of thyme liquid extracts. Planta Med 1981;41:129–135.

Van Den Broucke CO, Lemli JA. Spasmolytic activity of the flavonoids from Thymus vulgaris. Pharm Weekbl 1983;5:9–14.

Boskabady MH, Rakhshandah H, Moetamedshariati V. Bronchodilatory and anticholinergic effects of Carum copticum on isolated guinea pig tracheal chains. Med J Islam Rep Iran 1998;11:329–334.

Leonards B, Rampart M, Herman AG. Selective M3 muscarinic receptors inhibit smooth muscle contraction in rabit trachea without increasing the release of acetylcholine. J Pharmacol Exp Ther 1992;263:773-770.

Buckle DR, Arch JRS, Boering NE, Foster KA, Taylor JF, Taylor SG, et al. Relaxation effect of potassium channel activators BRL 38227 and Pinacidil on guinea-pig and human airway smooth muscle, and blockade of their effects by Glibenclamide and BRL 31660. Pulmon Pharmacol 1993;6:77–86.

Perez-Guerrero C, Suarez J, Herrera MD, Marhuenda E. Spasmolytic effect of tetrazepam on rat duodenum and guinea pig ileum. Pharmacol Res 1997;35:493–494.

McCaig D, DeJonckeere S. Effect of two Ca2+ modulator in normal and albumin sensitized guinea pig trachea. Eur J Pharmacol 1993;249:53–63.

Miyahara Y, Kizawa Y, Sano M, Murakami H. Effect of organic and inorganic Ca2+ antagonists on acetylcholine induced contraction in molluscan (Mytilus edulis) smooth muscle. Gen Pharmacol 1993;24:1419–1423.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Experimental study on the molecular mechanism of anthraquinone cathartics in inducing melanosis coli