Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid

Fang Lian; Hai-cui Wu; Zhen-gao Sun; Ying Guo; Lei Shi; Ming-yue Xue

doi:10.1007/s11655-014-1712-y

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Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid

OriginalPaper | Updated：2021-08-27

- Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid
- Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid
- 中国结合医学杂志（英文版） 2014年20卷第7期页码：503-509
- Affiliations：
  
  Integrative Medicine Research Centre of Reproduction and Heredity, the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Fund of China (No. 81072836), the Natural Science Foundation Project of Shandong Science and Technology Agency (No. 2009ZRB14306), and the Special Science and Technology Foundation for Doctoral Subject from Ministry of Education of China (No. 20093731110006)
- DOI：10.1007/s11655-014-1712-y
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-4-9，
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Lian, F., Wu, Hc., Sun, Zg. et al. Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid., Chin. J. Integr. Med. 20, 503–509 (2014). https://doi.org/10.1007/s11655-014-1712-y

Fang Lian, Hai-cui Wu, Zhen-gao Sun, et al. Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid[J]. Chinese Journal of Integrative Medicine, 2014,20(7):503-509.
Lian, F., Wu, Hc., Sun, Zg. et al. Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid., Chin. J. Integr. Med. 20, 503–509 (2014). https://doi.org/10.1007/s11655-014-1712-y DOI：

Fang Lian, Hai-cui Wu, Zhen-gao Sun, et al. Effects of Liuwei Dihuang Granule (六味地黄颗粒) on the outcomes of in vitro fertilization pre-embryo transfer in infertility women with Kidney-yin deficiency syndrome and the proteome expressions in the follicular fluid[J]. Chinese Journal of Integrative Medicine, 2014,20(7):503-509. DOI： 10.1007/s11655-014-1712-y.

摘要

To observe the effects of Liuwei Dihuang Granule (六味地黄颗粒

LDG) for tonifying Kidney (Shen) on the outcomes of in vitro fertilization pre-embryo transfer (IVF-ET) of infertility women with Kidneyyin deficiency syndrome and to explore its mechanism by detecting the proteome expression in the follicular fluid. Sixty-six infertility patients of Kidney-yin deficiency syndrome who would undergo IVF-ET

were randomly assigned to a treatment group and a control group according to a random number table

33 cases in each group. Another 33 cases of non-Kidney-yin deficiency syndrome was taken as a syndrome-control group. Besides Western routine therapy

LDG was given 3 menstrual cycles before IVF to the treatment group

and a placebo granule to the control and syndrome-control groups. The scores of Kidney-yin deficiency symptoms (sore waist and knees

dry vagina

dysphoria with feverish sensation in the chest

palms and soles

etc.) were assessed

the number of retrieved oocytes

rates of high quality oocytes and embryos

fertility rate and clinical pregnancy rate were recorded

and the follicular fluid was collected on the day when the ovum was picked up

the differential protein expression was detected using two-dimensional gel electrophoresis

and then

matrix assisted laser desorption ionization time-of flight mass spectrometry (MALDI-TOF-MS) was applied to identify the proteins. The syndrome score in the treatment group decreased significantly from 16.09±2.58 to 8.67±2.13

while it changed insignificantly in the control group

with a significant difference in the lowering score between the two groups (P<0.05); the high quality rates of oocytes and embryos and clinical pregnancy rate were all superior in the treatment group to the control group (82.29% vs 78.08%

76.76% vs 68.79%

63.64% vs 36.36%

all P<0.05). The protein expression map from the follicular fluid showed that compared with the control group

33 differential protein expressions were found in the syndrome-control group

among which 18 were down-regulated

and 15 up-regulated; in the treatment group 28 differential protein expressions were found

among which 15 were down-regulated

and 13 up-regulated. Through MALDI-TOF-MS

14 proteins were identified (P<0.05). For the infertility patients undergoing IVF

LDG could alleviate clinical symptoms

improve rates of high quality oocytes and embryos

so as to raise clinical pregnancy rate. The mechanism may be through regulating proteome expression in the follicular fluid to improve the developmental microenvironment for oocytes which would lead to a successful embryo implantation.

Abstract

To observe the effects of Liuwei Dihuang Granule (六味地黄颗粒

were randomly assigned to a treatment group and a control group according to a random number table

33 cases in each group. Another 33 cases of non-Kidney-yin deficiency syndrome was taken as a syndrome-control group. Besides Western routine therapy

LDG was given 3 menstrual cycles before IVF to the treatment group

and a placebo granule to the control and syndrome-control groups. The scores of Kidney-yin deficiency symptoms (sore waist and knees

dry vagina

dysphoria with feverish sensation in the chest

palms and soles

etc.) were assessed

the number of retrieved oocytes

rates of high quality oocytes and embryos

fertility rate and clinical pregnancy rate were recorded

and the follicular fluid was collected on the day when the ovum was picked up

the differential protein expression was detected using two-dimensional gel electrophoresis

and then

while it changed insignificantly in the control group

76.76% vs 68.79%

63.64% vs 36.36%

all P<0.05). The protein expression map from the follicular fluid showed that compared with the control group

33 differential protein expressions were found in the syndrome-control group

among which 18 were down-regulated

and 15 up-regulated; in the treatment group 28 differential protein expressions were found

among which 15 were down-regulated

and 13 up-regulated. Through MALDI-TOF-MS

14 proteins were identified (P<0.05). For the infertility patients undergoing IVF

LDG could alleviate clinical symptoms

improve rates of high quality oocytes and embryos

关键词

tonify KidneyKidney-yin deficiencyInfertilityLiuwei Dihuang Granuleprotein expression

Keywords

tonify KidneyKidney-yin deficiencyInfertilityLiuwei Dihuang Granuleprotein expression

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