Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats

Jia Guo; Tao Jin; Zi-qi Lin; Xiao-xiang Wang; Xiao-nan Yang; Qing Xia; Ping Xue

doi:10.1007/s11655-014-1750-5

Your Location：

Home >

Browse articles >

Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats

OriginalPaper | Updated：2021-08-27

- Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats
- Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats
- 中国结合医学杂志（英文版） 2015年21卷第1期页码：29-35
- Affiliations：
  
  Department of Integrated Traditional Chinese and Western Medicine, West China Hospital, Sichuan University,Chengdu,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072910), and the Science and Technology Supported Program of Sichuan Province, China (No. 2010SZ0068 and 2011SZ0291)
- DOI：10.1007/s11655-014-1750-5
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-5-10，
Scan for full text
Guo, J., Jin, T., Lin, Zq. et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats., Chin. J. Integr. Med. 21, 29–35 (2015). https://doi.org/10.1007/s11655-014-1750-5

Jia Guo, Tao Jin, Zi-qi Lin, et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats[J]. Chinese Journal of Integrative Medicine, 2015,21(1):29-35.
Guo, J., Jin, T., Lin, Zq. et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats., Chin. J. Integr. Med. 21, 29–35 (2015). https://doi.org/10.1007/s11655-014-1750-5 DOI：

Jia Guo, Tao Jin, Zi-qi Lin, et al. Effect of Chaiqin Chengqi Decoction (柴芩承气汤) on cholecystokinin receptor 1-mediated signal transduction of pancreatic acinar cells in acute necrotizing pancreatitis rats[J]. Chinese Journal of Integrative Medicine, 2015,21(1):29-35. DOI： 10.1007/s11655-014-1750-5.

摘要

To investigate the effect of Chaiqin Chengqi Decoction (柴芩承气汤

CQCQD) on cholecystokinin receptor 1 (CCKR1)-mediated signal transduction of pancreatic acinar cell in rats with acute necrotic pancreatitis (ANP). Twenty-seven Sprague-Dawley rats were randomized into three groups: the control group

the ANP group

and the CQCQD group (9 in each group). ANP rats were induced by two intraperitoneal injections of 8% L-arginine (pH=7.0

4.4 g/kg) over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD (CQCQD group) or physiological saline (control and ANP groups) at 2 h interval. And 6 h after induction

pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression

phospholipase C (PLC) and inositol-1

5-triphosphate (IP3)

and determination of fluorescence intensity (FI) as a measure of intracellular calcium ion concentration [Ca2+]i. The pancreatic histopathological score (6.2±1.1) and the levels of PLC (1

187.2±228.2 μg/mL) and IP3 (872.2±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control (2.8±0.4

682.5±121.8 μg/mL

518.4±115.8 μg/mL) and the CQCQD (3.8±0.8

905.3±78.5 μg/mL

611.0±42.5 μg/mL) groups (P<0.05). [Ca2+]i FI for the ANP group (34.8±27.0) was higher than that in the control (5.1±2.2) and CQCQD (12.6±2.5) groups (P<0.05). The expression of pancreatic acinar cell CCKR1 mRNA in the ANP group was up-regulated (expression ratio=1.761; P=0.024) compared with the control group. The expression of pancreatic acinar cell CCKR1 mRNA in the CQCQD group was down-regulated (expression ratio=0.311; P=0.035) compared with the ANP group. The ratio of gray values of the CCKR1 and β-actin in the ANP group (1.43±0.17) was higher than those in the control (0.70±0.15) and CQCQD (0.79±0.11) groups (P<0.05). Pancreatic acinar cell calcium overload of ANP induced by L-arginine was related to the up-regulated expressions of pancreatic acinar cell CCKR1 mRNA and protein. CQCQD can down-regulate expressions of pancreatic acinar cell CCKR1 mRNA and protein to reduce the PLC and IP3 of pancreatic acinar cells

relieving the calcium overload and reducing the pathological changes in rats with ANP.

Abstract

To investigate the effect of Chaiqin Chengqi Decoction (柴芩承气汤

the ANP group

and the CQCQD group (9 in each group). ANP rats were induced by two intraperitoneal injections of 8% L-arginine (pH=7.0

4.4 g/kg) over a 2-h period. Rats were treated with 1.5 mL/100 g body weight of CQCQD (CQCQD group) or physiological saline (control and ANP groups) at 2 h interval. And 6 h after induction

pancreatic tissues were collected for histopathological examination. Pancreatic acinar cells were isolated for determination of CCKR1 mRNA and protein expression

phospholipase C (PLC) and inositol-1

5-triphosphate (IP3)

and determination of fluorescence intensity (FI) as a measure of intracellular calcium ion concentration [Ca2+]i. The pancreatic histopathological score (6.2±1.1) and the levels of PLC (1

187.2±228.2 μg/mL) and IP3 (872.2±88.4 μg/mL) of acinar cells in the ANP group were higher than those in the control (2.8±0.4

682.5±121.8 μg/mL

518.4±115.8 μg/mL) and the CQCQD (3.8±0.8

905.3±78.5 μg/mL

relieving the calcium overload and reducing the pathological changes in rats with ANP.

关键词

Acute PancreatitisChaiqin Chengqi Decoctioncholecystokinin receptor 1Chinese Medicine

Keywords

Acute PancreatitisChaiqin Chengqi Decoctioncholecystokinin receptor 1Chinese Medicine

references

Williams JA. Receptor-mediated signal transduction pathways and the regulation of pancreatic acinar cell function. Curr Opin Gastroenterol 2008;24:573–579.

Cruzblanca H, Koh DS, Hille B. Bradykinin inhibits M current via phospholipase C and Ca2+ release from IP3-sensitive Ca2+ stores in rat sympathetic neurons. Proc Natl Acad Sci USA 1998;95:7151–7156.

Husain SZ, Prasad P, Grant WM, Kolodecik TR, Nathanson MH, Gorelick FS. The ryanodine receptor mediates early zymogen activation in pancreatitis. Proc Natl Acad Sci USA 2005;102:14386–14391.

Ward JB, Jenkins SA, Sutton R, Petersen OH. Is an elevated concentration of acinar cytosolic free ionised calcium the trigger for acute pancreatitis? Lancet 1995;346:1016–1019.

Griesbacher T, Rainer I, Heinemann A, Groisman D. Haemodynamic and exocrine effects of caerulein at submaximal and supramaximal levels on the rat pancreas: role of cholecystokinin receptor subtypes. Pancreatology 2006;6:65–75.

Barrett TD, Yan W, Freedman JM, Lagaud GJ, Breitenbucher JG, Shankley NP. Role of CCK and potential utility of CCK1 receptor antagonism in the treatment of pancreatitis induced by biliary tract obstruction. British J Pharma 2008;8:1650–1658.

Bruce JI, Elliott AC. Oxidant-impaired intracellular Ca2+ signaling in pancreatic acinar cells: role of the plasma membrane Ca2+-ATPase. Am J Physiol Cell Physiol 2007;293:C938–C950.

Yang F, Wang Y, Sternfeld L, Rodriguez JA, Ross C, Hayden MR, et al. The role of free fatty acids, pancreatic lipase and Ca+ signaling in injury of isolated acinar cells and pancreatitis model in lipoprotein lipase-deficient mice. Acta Physiol (Oxf) 2009;195:13–28.

Williams JA, Chen X, Sabbatini ME. Small G proteins as key regulators of pancreatic digestive enzyme secretion. Am J Physiol Endocrinol Metab 2009;296:E405–E414.

Williams JA. Receptor biology and intracellular regulatory mechanisms in pancreatic acinar cells. Curr Opin Gastroenterol 2002;18:529–535.

Streb H, Irvine RF, Berridge MJ, Schulz I. Release of Ca2+ from a nonmitochondrial intracellular store in pancreatic acinar cells by inositol 1,4,5-trisphosphate. Nature 1983;306:67–69.

González A, Schmida A, Sternfelda L, Krausea E, Salidob GM, Schulz I. Cholecystokinin-evoked Ca2+ waves in isolated mouse pancreatic acinar cells are modulated by activation of cytosolic phospholipase A2, phospholipase D, and protein kinase C. Biochem Biophys Res Commun 1999;261:726–733.

Rakonczay Z Jr, Takács T, Mándi Y, Iványi B, Varga S, Pápai G, et al. Water immersion Pretreatment decreases pro-inflammatory cytokine production in cholecystokininoctapeptide-induced acute pancreatitis in rats: possible role of HSP72. Int J HyPerthermia 2001;17:520–535.

Kanemitsu D, Sakagami J, Motoyoshi T, Nakajima T, Kataoka K. Effects of the cholecystokinin A receptor antagonist loxiglumide on the proliferation and cell cycle time of pancreatic acinar cells in rats. Pancreas 2006;32:190–196.

Liu XB, Jiang JM, Huang ZW, Tian BL, Hu WM, Xia Q, et al. Clinical study on the treatment of severe acute pancreatitis by integrated traditional Chinese medicine and Western medicine. J Sichuan Univ (Med Sci ed, Chin) 2004;35:204–208.

Deng LH, Yang XN, Xia Q. Protective effects of Chaiqin Chengqi Decoction on isolated pancreatic acinar cell in acute pancreatitis and the mechaniams. J Chin Integr Med (Chin) 2008;6:176–179.

Xue P, Deng LH, Zhang ZD, Yang XN, Xia Q, Xiang DK, et al. Effect of Chaiqinchengqi Decoction on sarco/endoplasmic reticulum Ca2+-ATPase mRNA expression of pancreatic tissues in acute pancreatitis rats. World J Gastroenterol 2008;14:2343–2348.

Ren J, Luo Z, Tian F, Wang Q, Li K, Wang C. Hydrogenrich saline reduces the oxidative stress and relieves the severity of trauma-induced acute pancreatitis in rats. J Trauma Acute Care Surg 2012;72:1555–1561.

González A, Camello PJ, Pariente JA, Salido GM. Free cytosolic calcium levels modify intracellular pH in rat pancreatic acini. Biochem Biophys Res Commun 1997;230:652–656.

Zhang XP, Tian H, Lai YH, Chen L, Zhang L, Cheng QH, et al. Protective effects and mechanisms of Baicalin and octreotide on renal injury of rats with severe acute pancreatitis. World J Gastroenterol 2007;13:5079–5089.

Schmidt J, Rattner DW, Lewandrowski K, Compton CC, Mandavilli U, Knoefel WT, et al. A better model of acute pancreatitis for evaluating therapy. Ann Surg 1992;215:44–56.

Chen CC, Wang SS, Tsay SH, Lee FY, Wu SL, Lu RH, et al. A model of experimental acute necrotizing pancreatitis. National Med J Chin (Chin) 1995;56:373–379.

Pandol SJ, Periskic S, Gukovsky I, Zaninovic V, Jung Y, Zong Y, et al. Ethanol diet increases the sensitivity of rats to pancreatitis induced by cholecystokinin octapeptide. Gastroenterology 1999;117:706–716.

Steinle AU, Weidenbach H, Wagner M, Adler G, Schmid RM. NF-κB/Rel activation in cerulein pancreatitis. Gastroenterology 1999;116:420–430.

Berna MJ, Jensen RT. Role of CCK/Gastrin receptors in gastrointestinal/metabolic diseases and results of human studies using Gastrin/CCK receptor agonists/antagonists in these diseases. Curr Topics Med Chem 2007;7:1211–1231.

Kim JK, Kim KH, Lee JA, Namkung W, Sun AQ, Ananthanarayanan M, et al. Transporter-mediated bile acid uptake causes Ca2+-dependent cell death in rat pancreatic acinar cells. Gastroenterology 2002;122:1941–1953.

Frick TW, Fernandez-del Castillo C, Bimmlerb D, Warshawa AL. Elevated calcium and activation of trypsinogen in rat pancreatic acini. Gut 1977;41:339–343.

Sahin-Tóth M, Tóth M. High-affinity Ca2+ blinding inhabits autoactivivation of rat trypsinogen. Biochem Biophys Res Commun 2000;275:668–671.

Waterford SD, Kolodecik TR, Thrower EC, Gorelick FS. Vacuolar ATPase regulates zymogen activation in pancreatic acini. J Biol Chem 2005;280:5430–5434.

Jung WS, Chae YS, Kim DY, Seo SW, Park HJ, Bae GS, et al. Gardenia jasminoides protects against cerulein-induced acute pancreatitis. World J Gastroenterol 2008;14:6188–6194.

Berna MJ, Tapia JA, Sancho V, Jensen RT. Progress in developing cholecystokinin (CCK)/gastrin receptor ligands that have therapeutic potential. Curr Opin Pharmacol 2007;7:583–592.

Gonzalez A, Pariente JA, Salido GM. Ethanol impairs calcium homeostasis following CCK-8 stimulation in mouse pancreatic acinar cells. Alcohol 2008;42:565–573.

Li GW, Wang QS, Hao JH, Xing WJ, Guo J, Li HZ, et al. The functional expression of extracellular calcium-sensing receptor in rat pulmonary artery smooth muscle cells. J Biomed Sci 2011;18:16.

Futatsugi A, Nakamura T, Yamada MK, Ebisui E, Nakamura K, Uchida K, et al. IP3 receptor types 2 and 3 mediate exocrine secretion underlying energy metabolism. Science 2005;309:2232–2234.

Rivera-Barreno R, Del Castillo-Vaquero A, Salido GM, Gonzalez A. Effect of cinnamtannin B-1 on cholecystokinin-8-evoked responses in mouse pancreatic acinar cells. Clin Exp Pharmacol Physiol 2010;37:980–988.

FULL TEXT LINK

More>

浏览量

Downloads

CSCD

文章被引用时，请邮件提醒。

Submit

工具集

关联资源

Changes of neuronal acetylcholine receptor alpha 7 of peritoneal macrophage in experimental acute pancreatitis treated by Chaiqin Chengqi Decoction (柴芩承气汤)

Observation on effect of yi’an decoction in treating 65 patients of acute pancreatitis) in treating 65 patients of acute pancreatitis

Future view and development of immunology: Exploring the immunology based on Chinese medicine and culture

Cost-effectiveness analysis of combined Chinese medicine and Western medicine for ischemic stroke patients

Objective tongue inspection on 142 liver cancer patients with damp-heat syndrome