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Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction
Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction
- 中国结合医学杂志（英文版） 2015年21卷第1期 页码：58-66
- Affiliations：
1. Institute of Integrative Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
2. Institute of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
3. Institute of Reproductive Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology,Wuhan,China
- Author bio：
- Funds：Supported by National Nature Science Foundation of China (No. S-049-09-10-20), and National Basic Research Program of China (973 Program, No. 2011CB505203)
DOI：10.1007/s11655-014-1755-0
中图分类号：纸质出版日期：2015，
网络出版日期：2014-5-6，
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Xiong, F., Gui, J., Yang, W. et al. Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction., Chin. J. Integr. Med. 21, 58–66 (2015). https://doi.org/10.1007/s11655-014-1755-0
Fan Xiong, Juan Gui, Wei Yang, et al. Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction[J]. Chinese Journal of Integrative Medicine, 2015,21(1):58-66.
Xiong, F., Gui, J., Yang, W. et al. Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction., Chin. J. Integr. Med. 21, 58–66 (2015). https://doi.org/10.1007/s11655-014-1755-0 DOI：
Fan Xiong, Juan Gui, Wei Yang, et al. Effects of acupuncture on progesterone and prolactin in rats of embryo implantation dysfunction[J]. Chinese Journal of Integrative Medicine, 2015,21(1):58-66. DOI： 10.1007/s11655-014-1755-0.
摘要
To investigate the effect of acupuncture on progesterone (P4) and prolactin (PRL) in rats of embryo implantation dysfunction (EID). On the first day of pregnancy
72 female Wistar rats were randomly allocated into the normal group
the EID model group
the acupuncture group and the P4 group (18 in each group). The normal group was injected sesame oil
while the other three groups were given mifepristone to establish the EID model. The acupuncture group and the P4 group were given treatment of acupuncture and P4 injection
respectively. The serum of P4 and PRL were detected by radioimmunoassay
and the mRNA and protein expressions of P4 receptor (PR) and PRL receptor (PRLR) were detected using real-time polymerase chain reaction and immunohistochemical method
respectively. Compared with the normal group
the serum levels of P4 and PRL as well as the mRNA and protein expression levels of PR and PRLR in the EID model group were significantly lowered (P<0.01 or P<0.05). The above indices in the acupuncture group and the P4 group were significantly elevated compared with the EID model group (P<0.01 or P<0.05). Acupuncture can promote embryo implantation effectively
which might be related to the effects of acupuncture on upregulating the P4 and PRL levels in serum and the PR and PRLR expression levels in rats.
Abstract
To investigate the effect of acupuncture on progesterone (P4) and prolactin (PRL) in rats of embryo implantation dysfunction (EID). On the first day of pregnancy
72 female Wistar rats were randomly allocated into the normal group
the EID model group
the acupuncture group and the P4 group (18 in each group). The normal group was injected sesame oil
while the other three groups were given mifepristone to establish the EID model. The acupuncture group and the P4 group were given treatment of acupuncture and P4 injection
respectively. The serum of P4 and PRL were detected by radioimmunoassay
and the mRNA and protein expressions of P4 receptor (PR) and PRL receptor (PRLR) were detected using real-time polymerase chain reaction and immunohistochemical method
respectively. Compared with the normal group
the serum levels of P4 and PRL as well as the mRNA and protein expression levels of PR and PRLR in the EID model group were significantly lowered (P<0.01 or P<0.05). The above indices in the acupuncture group and the P4 group were significantly elevated compared with the EID model group (P<0.01 or P<0.05). Acupuncture can promote embryo implantation effectively
which might be related to the effects of acupuncture on upregulating the P4 and PRL levels in serum and the PR and PRLR expression levels in rats.
关键词
acupunctureProgesteroneProlactinprogesterone receptorprolactin receptorembryo implantation dysfunction
Keywords
acupunctureProgesteroneProlactinprogesterone receptorprolactin receptorembryo implantation dysfunction
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