Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

Lu-ping Tang; Wei Xiao; Yi-fang Li; Hai-bo Li; Zhen-zhong Wang; Xin-sheng Yao; Hiroshi Kurihara; Rong-rong He

doi:10.1007/s11655-014-1758-x

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Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats

OriginalPaper | Updated：2021-08-27

- Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats
- Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats
- 中国结合医学杂志（英文版） 2014年20卷第8期页码：591-599
- Affiliations：
  
  1. Pharmacy College, Jinan University,Guangzhou,China
  2. State Key Laboratory of New-Tech for Chinese Medicine Pharmaceutical Process,Jiangsu Province,Lianyungang,China
- Author bio：
- Funds：
  
  Supported by Distinguished Trans-Century Training Program Foundation for the Talents of the State Education Commission, China (No. NCET-12-0678), Natural Science Foundation of Guangdong Province, China (No. S20120011316), Science and Technology Program of Guangzhou, China (No. 2012J22000073), Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (No. LYM11023)
- DOI：10.1007/s11655-014-1758-x
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-6-10，
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Tang, Lp., Xiao, W., Li, Yf. et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats., Chin. J. Integr. Med. 20, 591–599 (2014). https://doi.org/10.1007/s11655-014-1758-x

Lu-ping Tang, Wei Xiao, Yi-fang Li, et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats[J]. Chinese Journal of Integrative Medicine, 2014,20(8):591-599.
Tang, Lp., Xiao, W., Li, Yf. et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats., Chin. J. Integr. Med. 20, 591–599 (2014). https://doi.org/10.1007/s11655-014-1758-x DOI：

Lu-ping Tang, Wei Xiao, Yi-fang Li, et al. Anti-inflammatory effects of Reduning Injection (热毒宁注射液) on lipopolysaccharide-induced acute lung injury of rats[J]. Chinese Journal of Integrative Medicine, 2014,20(8):591-599. DOI： 10.1007/s11655-014-1758-x.

摘要

To evaluate the protective effects of Reduning Injection (热毒宁注射液

RDN)

a patent Chinese medicine

on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and its underlying mechanisms of action. Sixty male Sprague-Dawley rats were randomly divided into 6 groups

including normal control

model

dexamethasone (DEX

5 mg/kg)

RDN-H (720 mg/kg)

RDN-M (360 mg/kg) and RDN-L (180 mg/kg) groups

with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge

lung tissues and bronchoalveolar lavage fluid (BALF) were collected

and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination

measurement of gene and protein expressions

superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. In vivo pretreatment of RDN (360

720 mg/kg) significantly reduced the weight of wet to dry (W/D) ratio of lung

protein content in BALF

and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile

RDN enormously decreased BALF total inflammatory cells

especially neutrophil and macrophage cell numbers. Moreover

RDN increased SOD activity

inhibited MPO activity

alleviated LPS-induced tumor neurosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) expression in lung tissues. Furthermore

RDN (720 mg/kg) efficiently weakened nuclear factorkappa B (NF-κB) gene and protein expression. Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration

lowering MPO activity

TNF-α and iNOS gene expression by inhibiting NF-κB activity in LPS-induced ALI.

Abstract

To evaluate the protective effects of Reduning Injection (热毒宁注射液

RDN)

a patent Chinese medicine

on lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats and its underlying mechanisms of action. Sixty male Sprague-Dawley rats were randomly divided into 6 groups

including normal control

model

dexamethasone (DEX

5 mg/kg)

RDN-H (720 mg/kg)

RDN-M (360 mg/kg) and RDN-L (180 mg/kg) groups

with 10 rats in each group. Rats were challenged with intravenous injection of LPS 1 h after intraperitoneal treatment with RDN or DEX. At 6 h after LPS challenge

lung tissues and bronchoalveolar lavage fluid (BALF) were collected

and the number of inflammatory cells was determined. The right lungs were collected for histopathologic examination

measurement of gene and protein expressions

superoxide dismutase (SOD) and myeloperoxidase (MPO) activities. In vivo pretreatment of RDN (360

720 mg/kg) significantly reduced the weight of wet to dry (W/D) ratio of lung

protein content in BALF

and led to remarkable attenuation of LPS-induced histopathological changes in the lungs. Meanwhile

RDN enormously decreased BALF total inflammatory cells

especially neutrophil and macrophage cell numbers. Moreover

RDN increased SOD activity

inhibited MPO activity

alleviated LPS-induced tumor neurosis factor-α (TNF-α) and inducible nitric oxide synthase (iNOS) expression in lung tissues. Furthermore

RDN (720 mg/kg) efficiently weakened nuclear factorkappa B (NF-κB) gene and protein expression. Anti-inflammatory effects of RDN was demonstrated to be preventing pulmonary neutrophil infiltration

lowering MPO activity

TNF-α and iNOS gene expression by inhibiting NF-κB activity in LPS-induced ALI.

关键词

Acute Lung Injurylipopolysaccharideneutrophilschemokinenuclear factor-kappa BReduning InjectionChinese Patent Medicine

Keywords

Acute Lung Injurylipopolysaccharideneutrophilschemokinenuclear factor-kappa BReduning InjectionChinese Patent Medicine

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