Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats

Ying Xu; Guo-jing Dai; Qian Liu; Xiao-ping Ma; Li Li; Wei-heng Chen; Na Lin

doi:10.1007/s11655-014-1761-2

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Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats

OriginalPaper | Updated：2021-08-27

- Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats
- Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats
- 中国结合医学杂志（英文版） 2014年20卷第8期页码：600-609
- Affiliations：
  
  1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences,Beijing,China
  2. Wangjing Hospital, China Academy of Chinese Medical Sciences,Beijing,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81072900)
- DOI：10.1007/s11655-014-1761-2
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-8-5，
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Xu, Y., Dai, Gj., Liu, Q. et al. Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats., Chin. J. Integr. Med. 20, 600–609 (2014). https://doi.org/10.1007/s11655-014-1761-2

Ying Xu, Guo-jing Dai, Qian Liu, et al. Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(8):600-609.
Xu, Y., Dai, Gj., Liu, Q. et al. Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats., Chin. J. Integr. Med. 20, 600–609 (2014). https://doi.org/10.1007/s11655-014-1761-2 DOI：

Ying Xu, Guo-jing Dai, Qian Liu, et al. Effect of Ermiao Recipe (二妙方) with medicinal guide Angelicae Pubescentis Radix on promoting the homing of bone marrow stem cells to treat cartilage damage in osteoarthritis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(8):600-609. DOI： 10.1007/s11655-014-1761-2.

摘要

To investigate the effect of Ermiao Recipe (二妙方

EMR) with medicinal guide Angelicae Pubescentis Radix (APR) on the homing of bone marrow stem cells (BMSCs) to focal zone in osteoarthritis (OA) rats. Forty-eight Sprague-Dawley rats were randomly assigned to the sham-operated

model

EMR

and EMR plus APR groups (12 rats in each group). The OA rat model was induced by anterior cruciate ligament transection and medial meniscus resection. All rats were injected with recombinant human granulocyte colonystimulating factor [rhG-CSF

30 μg/(kg·d) for continuous 7 days]

and rats in the EMR and EMR plus APR groups were treated with EMR or EMR plus APR at 1.6 or 1.9 g/(kg·d) for 3 or 6 weeks

respectively. Cartilage histopathologic changes were observed by hematoxylin and eosin staining. Chondrocytes apoptosis and cartilage matrix components were tested by transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay and special staining. Interleukin-1β (IL-1 β)

tumor necrosis factor α (TNF-α)

bone morphogenetic protein 2 (BMP-2)

and transforming growth factor beta-1 (TGF-β1) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay assay. Matrix metalloproteinase (MMP)-13

tissue inhibitors of metalloproteinase (TIMP)-1

bromodeoxyuridine (BrdU)

cluster of differentiation 34 (CD34)

and stromal cell derived factor 1 (SDF-1) were measured by immunohistochemistry assay. EMR and EMR plus APR significantly inhibited articular cartilage damage and synovium inflammation in OA rats at 3 or 6 weeks of treatment

the most obvious changes in these parameters were found in the EMR plus APR group. At 6 weeks

compared with EMR treatment

EMR plus APR remarkably inhibited chondrocytes apoptosis and the release of IL-1β and TNF-α

obviously decreased MMP-13 expression

and significantly increased expressions of proteoglycan

collagen

type II collagen and TIMP-1

serum levels of BMP-2 and TGF-β1 as well as expressions of BrdU

CD34 and SDF-1 in cartilage articular (P<0.01 or P<0.05). The medicinal guide APR improved the therapeutic effects of EMR on OA rats by promoting directional homing of BMSCs to focal zone.

Abstract

To investigate the effect of Ermiao Recipe (二妙方

model

EMR

30 μg/(kg·d) for continuous 7 days]

and rats in the EMR and EMR plus APR groups were treated with EMR or EMR plus APR at 1.6 or 1.9 g/(kg·d) for 3 or 6 weeks

tumor necrosis factor α (TNF-α)

bone morphogenetic protein 2 (BMP-2)

and transforming growth factor beta-1 (TGF-β1) in serum were detected by enzyme-linked immunosorbent assay or radioimmunoassay assay. Matrix metalloproteinase (MMP)-13

tissue inhibitors of metalloproteinase (TIMP)-1

bromodeoxyuridine (BrdU)

cluster of differentiation 34 (CD34)

the most obvious changes in these parameters were found in the EMR plus APR group. At 6 weeks

compared with EMR treatment

EMR plus APR remarkably inhibited chondrocytes apoptosis and the release of IL-1β and TNF-α

obviously decreased MMP-13 expression

and significantly increased expressions of proteoglycan

collagen

type II collagen and TIMP-1

serum levels of BMP-2 and TGF-β1 as well as expressions of BrdU

CD34 and SDF-1 in cartilage articular (P<0.01 or P<0.05). The medicinal guide APR improved the therapeutic effects of EMR on OA rats by promoting directional homing of BMSCs to focal zone.

关键词

Ermiao Recipeosteoarthritisbone marrow stem cellAngelicae Pubescentis Radixmedicinal guideChinese Medicine

Keywords

Ermiao Recipeosteoarthritisbone marrow stem cellAngelicae Pubescentis Radixmedicinal guideChinese Medicine

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