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Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs
Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs
- 中国结合医学杂志（英文版） 2015年21卷第7期 页码：523-529
- Affiliations：
1. Advance Institute of Biotech & Paramedical Sciences, Naramau, Kanpur,India
2. Institute for Laboratory Medicine Clinical Chemistry and Pathobiochemistry, Charite University of Medicine, Campus Benjamin Franklin, Hindenburgdamm,Berlin,Germany
- Author bio：
- Funds：
DOI：10.1007/s11655-014-1771-5
中图分类号：纸质出版日期：2015，
网络出版日期：2014-12-16，
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Mishra, A., Mishra, S., Bhargav, S. et al. Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs., Chin. J. Integr. Med. 21, 523–529 (2015). https://doi.org/10.1007/s11655-014-1771-5
Ashish Mishra, Shilpi Mishra, Shilpi Bhargav, et al. Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs[J]. Chinese Journal of Integrative Medicine, 2015,21(7):523-529.
Mishra, A., Mishra, S., Bhargav, S. et al. Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs., Chin. J. Integr. Med. 21, 523–529 (2015). https://doi.org/10.1007/s11655-014-1771-5 DOI：
Ashish Mishra, Shilpi Mishra, Shilpi Bhargav, et al. Microwave assisted extraction, antioxidant potential and chromatographic studies of some Rasayana drugs[J]. Chinese Journal of Integrative Medicine, 2015,21(7):523-529. DOI： 10.1007/s11655-014-1771-5.
摘要
To study and compare the conventional extraction procedure with microwave assisted extraction (MAE) for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Three Ayurvedic rasayana plants Allium sativum Linn.
Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree
extraction solvent
effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2
2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic (TLC) analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. The total extractive value for Allium sativum Linn. was 36.95% (w/w) and 49.95% (w/w) for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% (w/w) compared to 42% (w/w) in ethanol (50% v/v). Percent yield of Inula racemosa Hook. in aqueous extract was found to be 27.55% (w/w) which was better than ethanol extract (50%) where the yield was 25.95% (w/w). Upon antioxidant activity evaluation. sativum extract showed an absorbance of 0.980±0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by. ceiba Linn. 0.825±0.98 and. racemosa Hook. with 0.799±2.01 at a concentration of 500 μg. TLC based standardization of. sativum Linn. extract shows single spot with Rf value of 0.38
B. ceiba Linn. extract shows Rf values were 0.23
0.58
0.77
0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three drugs have high antioxidant potential and a TLC profiling similar to reported ones. The presence of fructan type polysaccharide can be further utilized for bioactivity directed fractionation and evaluation of immunomodulatory activity.
Abstract
To study and compare the conventional extraction procedure with microwave assisted extraction (MAE) for some Ayurvedic Rasayana drugs and to evaluate their antioxidant potential and carry out the characterization of extracts by thin layer chromatography. Three Ayurvedic rasayana plants Allium sativum Linn.
Bombax ceiba Linn. and Inula racemosa Hook. were evaluated for an improved MAE methodology by determining the effects of grinding degree
extraction solvent
effect of dielectric constant and duration of time on the extractive value. Antioxidant potential of all three drugs was evaluated with 2
2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity and reducing power was determined by using Gallic acid as standard. Further thin layer chromatographic (TLC) analysis was performed on pre-activated Silica Gel G plates and Rf value were compared with those reported for the important biomarkers. The total extractive value for Allium sativum Linn. was 36.95% (w/w) and 49.95% (w/w) for ethanol extraction respectively. In case of Bombax ceiba Linn. the yield of aqueous extract by MAE was 50% (w/w) compared to 42% (w/w) in ethanol (50% v/v). Percent yield of Inula racemosa Hook. in aqueous extract was found to be 27.55% (w/w) which was better than ethanol extract (50%) where the yield was 25.95% (w/w). Upon antioxidant activity evaluation. sativum extract showed an absorbance of 0.980±0.92 at concentration of 500 μg with maximum reducing capacity. This was followed by. ceiba Linn. 0.825±0.98 and. racemosa Hook. with 0.799±2.01 at a concentration of 500 μg. TLC based standardization of. sativum Linn. extract shows single spot with Rf value of 0.38
B. ceiba Linn. extract shows Rf values were 0.23
0.58
0.77
0.92 and I. racemosa Hook. extract spot had a Rf value of 0.72. A significant improvement in extractive values was observed as a factor of time and other advantages by using MAE technology. All three drugs have high antioxidant potential and a TLC profiling similar to reported ones. The presence of fructan type polysaccharide can be further utilized for bioactivity directed fractionation and evaluation of immunomodulatory activity.
关键词
antioxidant potentialAyurvedic RasayanaAllinm sativum Linn.Bombax ceibaInula racemosamicrowave assisted extraction
Keywords
antioxidant potentialAyurvedic RasayanaAllinm sativum Linn.Bombax ceibaInula racemosamicrowave assisted extraction
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