Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis

Ying-hong Li; Zheng-zhi Wu; Mei-qun Cao; Ming Li; Ke-huan Sun; Min Yang; Man-yin Chen; Andrew C. J. Huang

doi:10.1007/s11655-014-1773-3

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Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis

OriginalPaper | Updated：2021-08-27

- Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis
- Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis
- 中国结合医学杂志（英文版） 2015年21卷第2期页码：123-131
- Affiliations：
  
  1. The First Affiliated Hospital of Shenzhen University,Guangdong Province,Shenzhen,China
  2. The Second Clinical Medical College of Jinan University,Guangdong Province,Shenzhen,China
  3. Larry Hillblom Islet Research Center, David Geffen School of Medicine, UCLA,Los Angeles,USA
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 39770890)
- DOI：10.1007/s11655-014-1773-3
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-7-31，
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Li, Yh., Wu, Zz., Cao, Mq. et al. Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis., Chin. J. Integr. Med. 21, 123–131 (2015). https://doi.org/10.1007/s11655-014-1773-3

Ying-hong Li, Zheng-zhi Wu, Mei-qun Cao, et al. Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis[J]. Chinese Journal of Integrative Medicine, 2015,21(2):123-131.
Li, Yh., Wu, Zz., Cao, Mq. et al. Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis., Chin. J. Integr. Med. 21, 123–131 (2015). https://doi.org/10.1007/s11655-014-1773-3 DOI：

Ying-hong Li, Zheng-zhi Wu, Mei-qun Cao, et al. Effect of Tiantai No.1 (天泰1号) on gene expression profiles in hippocampus of Alzheimer’s disease rats by bioinformatic analysis[J]. Chinese Journal of Integrative Medicine, 2015,21(2):123-131. DOI： 10.1007/s11655-014-1773-3.

摘要

To study the effect of Tiantai No. 1 (天泰1号) on gene expression profile in hippocampus of Alzheimer’s disease (AD) rat

molecular genetic target points of the effect of this drug were defined

its molecular genetic pharmacodynamic mechanism of anti-AD was further explored at molecular gene level

and a scientific basis was provided for its clinical availability and promotion. Thirty male Sprague-Dawley rats were divided into three groups with 10 rats per group: sham-operation group

model group and Tiantai No. 1 group. Sterile surgical procedure was applied

the model group with bilateral hippocampal injection of Aβ1–40 was established

and normal saline was used instead of Aβ1–40 in the sham-operation group. One week after the models was made

rats were administered by gastric lavage once every day for three consecutive weeks. The rats of the sham-operation group and the model group were daily fed with purified water by lavage; the rats of the Tiantai No.1 group treated group were administered with Tiantai No.1 by lavage. Total RNAs of hippocampus tissues were extracted with Trizol

the changes of hippocampus gene expression profiles in the above three groups were analyzed by using Affymetrix rat whole genome expression profile microarray. Microarray analysis showed that

compared with the sham-operation group

the hippocampus of the model group had 50 up-regulated genes with significant difference (fold change >2)

and 21 down-regulated genes with significant difference (fold change <0.5); compared with the hippocampus of the model group

the hippocampus of the Tiantai No. 1 group was found to have 5 up-regulated genes with significant difference (fold change >2) and 20 down-regulated genes with significant difference (fold change <0.5). The functions of differentially expressed genes of the groups were involved in nervous system’s development

neuronic differentiation and function-regulation

cellular growth and differentiation and apoptosis

synaptic occurrence and plasticity

inflammation and immune response

ion channels/transporters

cellular signal transduction

cellular material/energy metabolism and so on. Tiantai No. 1 can regulate hippocampal function

and further regulate the brain function of animals in multiple gene target points by a number of ways.

Abstract