Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

Hu Yan; You-xin Su; Xue-yi Lin; Bao-jun Chen; Qing Zhang; Zi-yi Zhang; Yi-ru Wang; Ya-nan Li; Mei-li Lu; Zhen He; Lu Sheng; Wen-ting Wang

doi:10.1007/s11655-014-1801-y

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Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway

OriginalPaper | Updated：2021-08-27

- Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway
- Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway
- 中国结合医学杂志（英文版） 2014年20卷第5期页码：353-359
- Affiliations：
  
  Fujian University of Traditional Chinese Medicine,Fuzhou,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81072828)
- DOI：10.1007/s11655-014-1801-y
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-1-30，
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Yan, H., Su, Yx., Lin, Xy. et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway., Chin. J. Integr. Med. 20, 353–359 (2014). https://doi.org/10.1007/s11655-014-1801-y

Hu Yan, You-xin Su, Xue-yi Lin, et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(5):353-359.
Yan, H., Su, Yx., Lin, Xy. et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway., Chin. J. Integr. Med. 20, 353–359 (2014). https://doi.org/10.1007/s11655-014-1801-y DOI：

Hu Yan, You-xin Su, Xue-yi Lin, et al. Zhuanggu Jianxi Decoction (壮骨健膝方) limits interleukin-1 β-induced degeneration chondrocytes via the caveolin-p38 MAPK signal pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(5):353-359. DOI： 10.1007/s11655-014-1801-y.

摘要

To evaluate the effect of Zhuanggu Jianxi Decoction (壮骨健膝方

ZGJXD) on interleukin-1 β (IL-1 β)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway

investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Serum pharmacology was applied in the present study

where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum (ZGJXD-S) was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL-1 β stimulation

the degeneration of CDs was verified by inverted microscope

toluidine blue stain and type II collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum

ZGJXD-S

together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h

caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting

and the mRNA expression of IL-1 β

tumor necrosis factor α (TNF-α)

matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction. IL-1 β stimulation induced degeneration of CDs

increased caveolin-1 expression and p38 phosphorylation

up-regulated the mRNA level of IL-1 β

TNF-α

MMP-3 and MMP-13. However

the IL-1 β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs. ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway

which might be one of the mechanisms that ZGJXD treats osteoarthritis.

Abstract

To evaluate the effect of Zhuanggu Jianxi Decoction (壮骨健膝方

ZGJXD) on interleukin-1 β (IL-1 β)-induced degeneration of chondrocytes (CDs) as well as the activation of caveolin-p38 mitogen-activated protein kinase (MAPK) signal pathway

investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Serum pharmacology was applied in the present study

the degeneration of CDs was verified by inverted microscope

toluidine blue stain and type II collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum

ZGJXD-S

together with or without SB203580 (a specific inhibitor of p38 MAPK) for 48 h

caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting

and the mRNA expression of IL-1 β

tumor necrosis factor α (TNF-α)

matrix metalloproteinase 3 (MMP-3) and MMP-13 were examined by real-time polymerase chain reaction. IL-1 β stimulation induced degeneration of CDs

increased caveolin-1 expression and p38 phosphorylation

up-regulated the mRNA level of IL-1 β

TNF-α

MMP-3 and MMP-13. However

which might be one of the mechanisms that ZGJXD treats osteoarthritis.

关键词

osteoarthritisZhuanggu Jianxi Decoctionchondrocyte degenerationcaveolin-p38 pathwayserum pharmacology

Keywords

osteoarthritisZhuanggu Jianxi Decoctionchondrocyte degenerationcaveolin-p38 pathwayserum pharmacology

references

Qiu GX, Rong GW, eds. Bone science. Beijing: Peking Union Medical College Press; 2012:506.

Zarubin T, Han Jh. Activation and signaling of the p38MAP kinase pathway. Cell Res 2005;15:11–18.

Wei L, Sun XJ, Wang Z, Chen Q. CD95-induced osteoarthritic chondrocyte apoptosis and necrosis: dependency on p38 mitogen-activated protein kinase. Arthritis Res Ther 2006;8:R37.

Liu W, Wu YH, Liu XY, Xue B, Shen W, Yang K. Metabolic regulatory and anti-oxidative effects of modified Bushen Huoxue Decoction on experimental rabbit model of osteoarthritis. Chin J Integr Med 2013;19:459–463.

Ye HZ, Zheng CS, Xu XJ, Wu MX, Liu XX. Potential synergistic and multitarget effect of herbal pair Chuanxiong Rhizome-Paeonia Albifora Pall on osteoarthritis disease: a computational pharmacology approach. Chin J Integr Med 2011;17:698–703.

Wang HM, Liu JN, Zhao Y. Progress on integrated Chinese and Western medicine in the treatment of osteoarthritis. Chin J Integr Med 2010;16:378–384.

Wu MX, Li XH, Lin MN, Jia XR, Mu R, Wan WR, et al. Clinical study on the treatment of knee osteoarthritis of Shen-Sui in sufficiency syndrome type by electroacupuncture. Chin J Integr Med 2010;16:291–297.

Xu SL, Su YX, Lin JR. A clinical study of 158 cases of osteitis decoction in the treatment of knee osteoarthritis. Chin J Orthopedics Traumatol Chin Med (Chin) 2001;9:45–46.

Huang P, Su YX. The efficacy observation of the Jianxi Zhitong Decoction therapy 63 patients of osteoarthritis with the knee joint dysfunction. Modern Rehabilitation 2001;5:120.

Wu BC, ed. Method and basic technology of medical animal experiment. Harbin: Heilongjiang People’s Publishing House 2008;8:184.

Wu ZL, Li XH, Wu GW, Ye HZ, Wu MX, Liu XX. Effects of serum containing Tougu Xiaotong Capsule to apoptosis pathway of chondrocyte mitochondrial. China J Tradit Chin Med Pharm (Chin) 2011;26:343–346.

Lawrence RC, Felson DT, Helmick CG, Arnold LM, Choi H, Deyo RA, et al. Estimates of the prevalence of arthritis and other rheumatic conditions in the United States. Part II. Arthritis Rheum 2008;58:26–35.

Zhang W, Nuki G, Moskowitz RW, Abramson S, Aitman RD, Arden NK, et al. OARSI recommendations for the management of hip and knee osteoarthritis: Part III: changes in evidence following systematic cumulative update of research published through January 2009. Osteoarthritis Cartilage 2010;18:476–499.

Matchaba P, Gitton X, Krammer G, Ehrsam E, Sloan VS, Olson M, et al. Cardiovascular safety of lumiracoxib: a metaanalysis of all randomized controlled trials >or =1 week and up to 1 year in duration of patients with osteoarthritis and rheumatoid arthritis. Clin Ther 2005;27:1196–1214.

Samuels J, Krasnokutsky S, Abramson SB. Osteoarthritis: a tale of three tissues. Bull NYU Hosp Jt Dis 2008;66:244–250.

Goldring MB, Otero M. Inflammation in osteoarthritis. Curr Opin Rheumatol 2011;23:471–478.

Kapoor M, Martel-Pelletier J, Lajeunesse D, Pelletier JP, Fahmi H. Role of proinflammatory cytokines in the pathophysiology of osteoarthritis. Nat Rev Rheumatol 2011;7:33–42.

Loeser RF, Erickson EA, Long DL. Mitogen-activated protein kinases as therapeutic targets in o steoarthritis. Curr Opin Rheumatol 2008;20:581–586.

Fan Z, Soder S, Oeheler S, Fundel K, Aiqner T. Activation of interleukin-1 signaling cascades in normal and osteoarthritic articular cartilage. Am J pathol 2007;171:938–946.

Schett G, Zwerina J, Firestein G. The p38 mitogen-activated protein kinase (MAPK) pathway in rheumatoid arthritis. Ann Rheum Dis 2008;67:909–916.

Lee JC, Laydon JT, McDonnell PC, Gallaqher TF, Kumar S, Green D, et al. A protein kinase involved in the regulation of inflammatory cytokine biosynthesis. Nature 1994;372:739–746.

Peifer C, Wagner G, Laufer S. New approaches to the treatment of inflammatory disorders small molecule inhibitors of p38 MAP kinase. Curr Top Med Chem 2006;6:113–149.

Schindler JF, Monahan JB, Smith WG. P38 pathway kinases as anti-inflammatory drug targets. J Dent Res 2007;86:800–811.

Cohen P. Targeting protein kinases for the development of anti-inflammatory drugs. Curr Opin Cell Biol 2009;21:317–324.

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