Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism

Yong Zhao; Bao-en Wang; Shu-wen Zhang; Shu-min Yang; Hong Wang; Ai-min Ren; En-tong Yi

doi:10.1007/s11655-014-1805-7

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Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism

OriginalPaper | Updated：2021-08-27

- Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism
- Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism
- 中国结合医学杂志（英文版） 2015年21卷第3期页码：211-216
- Affiliations：
  
  1. Department of Infectious Diseases, Beijing Friendship Hospital, Capital Medical University,Beijing,China
  2. School of Chemical Biology and Pharmaceutical Sciences, Capital Medical University,Beijing,China
- Author bio：
- Funds：
  
  Supported in part by a grant from Bureau of Personnel of Beijing (No. 100005)
- DOI：10.1007/s11655-014-1805-7
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-2-27，
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Zhao, Y., Wang, Be., Zhang, Sw. et al. Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism., Chin. J. Integr. Med. 21, 211–216 (2015). https://doi.org/10.1007/s11655-014-1805-7

Yong Zhao, Bao-en Wang, Shu-wen Zhang, et al. Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism[J]. Chinese Journal of Integrative Medicine, 2015,21(3):211-216.
Zhao, Y., Wang, Be., Zhang, Sw. et al. Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism., Chin. J. Integr. Med. 21, 211–216 (2015). https://doi.org/10.1007/s11655-014-1805-7 DOI：

Yong Zhao, Bao-en Wang, Shu-wen Zhang, et al. Isolation of antifungal compound from Paeonia suffruticosa and its antifungal mechanism[J]. Chinese Journal of Integrative Medicine, 2015,21(3):211-216. DOI： 10.1007/s11655-014-1805-7.

摘要

To isolate antifungal compound from Paeonia suffruticosa

and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1

6-penta-O-galloyl-beta-D-glucose (PGG). Peony (Paeonia suffruticosa) root bark (PRB) was separated by solvent extraction and purified by high performance liquid chromatography (HPLC) method using analytical and preparative reversed phase C18 column on the basis of bio-assay method. In order to investigate the antifungal mechanism of PGG

Yeasts were submitted to different concentrations [3 × minimum inhibition concentration (MIC)

0.3 × MIC] for 1 h under constant stirring at 30 °C

and transmission electron microscopy was performed. Based on the antifungal activity of PRB on Candida glabrata CBS138

the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry

1H nuclear magnetic resonance (NMR) analyses

with molecular weight of 940 and molecular formular as C41H32O26. Transmission electron microscopy showed that PGG degraded the cell wall envelope. The results suggest that PGG may be responsible for the antifungal activity of PRB by disrupting the structure of cell wall directly.

Abstract

To isolate antifungal compound from Paeonia suffruticosa

and to find the antifungal mechanisms by observing the ultrastructural modifications of yeasts in growth phase produced by 1

Yeasts were submitted to different concentrations [3 × minimum inhibition concentration (MIC)

0.3 × MIC] for 1 h under constant stirring at 30 °C

and transmission electron microscopy was performed. Based on the antifungal activity of PRB on Candida glabrata CBS138

the antifungal compound were isolated in ethyl acetate layer of PRB and identified as PGG by mass spectrometry

1H nuclear magnetic resonance (NMR) analyses

关键词

Paeonia suffruticosa12346-penta-O-galloyl-β-D-glucoseantifungal activity

Keywords

Paeonia suffruticosa12346-penta-O-galloyl-β-D-glucoseantifungal activity

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