Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

Lin-jie Li; Neng-wen Xu; Rui-lan Gao; Xiao-jie Lin; Hong-ying Qiu; Wei-hong Liu; Yang-jin Jin; Min-lei Zhao

doi:10.1007/s11655-014-1806-6

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Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells

OriginalPaper | Updated：2021-08-27

- Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells
- Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells
- 中国结合医学杂志（英文版） 2014年20卷第5期页码：381-386
- Affiliations：
  
  1. Department of Hematology, Lishui Central Hospital,Zhejiang Province,Lishui,China
  2. Institution of Hematology Research, The First Affiliated Hospital, Zhejiang Chinese Medical University,Hangzhou,China
- Author bio：
- Funds：
  
  Supported by Zhejiang Province Administration of Traditional Chinese Medicine (No. 2010ZA120) and Zhejiang Provincial Natural Science Foundation of China (No. Y207632)
- DOI：10.1007/s11655-014-1806-6
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-3-7，
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Li, Lj., Xu, Nw., Gao, Rl. et al. Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells., Chin. J. Integr. Med. 20, 381–386 (2014). https://doi.org/10.1007/s11655-014-1806-6

Lin-jie Li, Neng-wen Xu, Rui-lan Gao, et al. Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells[J]. Chinese Journal of Integrative Medicine, 2014,20(5):381-386.
Li, Lj., Xu, Nw., Gao, Rl. et al. Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells., Chin. J. Integr. Med. 20, 381–386 (2014). https://doi.org/10.1007/s11655-014-1806-6 DOI：

Lin-jie Li, Neng-wen Xu, Rui-lan Gao, et al. Effects of Danshen Injection (丹参注射液) on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells[J]. Chinese Journal of Integrative Medicine, 2014,20(5):381-386. DOI： 10.1007/s11655-014-1806-6.

摘要

To explore the effects of Danshen Injection (丹参注射液) on inhibition proliferation

inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells. The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology

analyzed by flow cytometry with annexin V and propidium iodide (PI) staining

and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile

the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot. The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner

with suppression rates from 19.46±2.31% to 50.20±5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells

the rates of annexin V positive cells increased obviously in a dose-dependent manner

as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection. Danshen Injection could not only significantly inhibit the proliferation

but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.

Abstract

To explore the effects of Danshen Injection (丹参注射液) on inhibition proliferation

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology

analyzed by flow cytometry with annexin V and propidium iodide (PI) staining

and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile

with suppression rates from 19.46±2.31% to 50.20±5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells

the rates of annexin V positive cells increased obviously in a dose-dependent manner

but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.

关键词

Danshen Injectionhuman erythroid leukemic cellproliferationapoptosismutant Janus kinase 2

Keywords

Danshen Injectionhuman erythroid leukemic cellproliferationapoptosismutant Janus kinase 2

references

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