Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells

Qian Chen; Qin Zhuang; Wei Mao; Xiao-ming Xu; Li-hui Wang; Hai-bing Wang

doi:10.1007/s11655-014-1810-x

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Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells

OriginalPaper | Updated：2021-08-27

- Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells
- Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells
- 中国结合医学杂志（英文版） 2014年20卷第10期页码：743-750
- Affiliations：
  
  1. Department of Cardiology, First Affiliated Hospital of Zhejiang Chinese Medical University,Hangzhou,China
  2. Lab, First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou,Central,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 81170270) and Medicine and Technology Program of Zhejiang Province (No. 2013KYB188)
- DOI：10.1007/s11655-014-1810-x
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-4-16，
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Chen, Q., Zhuang, Q., Mao, W. et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells., Chin. J. Integr. Med. 20, 743–750 (2014). https://doi.org/10.1007/s11655-014-1810-x

Qian Chen, Qin Zhuang, Wei Mao, et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells[J]. Chinese Journal of Integrative Medicine, 2014,20(10):743-750.
Chen, Q., Zhuang, Q., Mao, W. et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells., Chin. J. Integr. Med. 20, 743–750 (2014). https://doi.org/10.1007/s11655-014-1810-x DOI：

Qian Chen, Qin Zhuang, Wei Mao, et al. Inhibitory effect of cryptotanshinone on angiogenesis and Wnt/β-catenin signaling pathway in human umbilical vein endothelial cells[J]. Chinese Journal of Integrative Medicine, 2014,20(10):743-750. DOI： 10.1007/s11655-014-1810-x.

摘要

To investigate the anti-angiogenic effect of cryptotanshinone (CPT) on human umbilical vein endothelial cells (HUVECs) and the effect of CPT on Wnt/β-catenin signaling pathway. HUVECs were incubated with 0

2.5

and 20 μ mol/L CPT for detecting cell viability with dimethyl thiazolyl-2

5-diphenyltetrazolium bromide (MTT) assay. Then

HUVECs were incubated with 0

2.5

and 10 μ mol/L CPT for detecting endothelial cell migration

invasion

and tubular-like structure formation with wound healing

transwell invasion and matrigel tube formation assays

respectively. To gain insight into CPT-mediated signaling

the effects of CPT on T-cell factor/lymphocyte enhancer factor (TCF/LEF) transcription factors were detected by the Dual-luciferase reporter assay. Next

the nuclear expression of β-catenin was evaluated using Western blot and immunochemistry. Finally

vascular endothelial growth factor (VEGF) and cyclin D1

downstream proteins of the Wnt pathway were examined with Western blot. CPT dose-dependently suppressed endothelial cell viability

migration

invasion

and tubular-like structure formation. In particular

CPT blocked β-catenindependent transcription in HUVECs in a dose-dependent manner. In Western bolt

10 μ mol/L CPT decreased expression of β-catenin in nucleus of HUVECs (P<0.01). In immunohistochemistry

β-catenin was more potent in response to LiCl (an activator of the pathway) treatment. However

the signals were weaker in the nucleus of the CPT (10 μ mol/L) group

compared to the positive control. Also

VEGF and cyclin D1 were both eliminated by CPT in 5 and 10 μ mol/L doses (P<0.05). Our study supported the role of CPT as an angiogenic inhibitor

which may impact on the Wnt/β-catenin signaling pathway.

Abstract

2.5

and 20 μ mol/L CPT for detecting cell viability with dimethyl thiazolyl-2

5-diphenyltetrazolium bromide (MTT) assay. Then

HUVECs were incubated with 0

2.5

and 10 μ mol/L CPT for detecting endothelial cell migration

invasion

and tubular-like structure formation with wound healing

transwell invasion and matrigel tube formation assays

respectively. To gain insight into CPT-mediated signaling

the effects of CPT on T-cell factor/lymphocyte enhancer factor (TCF/LEF) transcription factors were detected by the Dual-luciferase reporter assay. Next

the nuclear expression of β-catenin was evaluated using Western blot and immunochemistry. Finally

vascular endothelial growth factor (VEGF) and cyclin D1

downstream proteins of the Wnt pathway were examined with Western blot. CPT dose-dependently suppressed endothelial cell viability

migration

invasion

and tubular-like structure formation. In particular

CPT blocked β-catenindependent transcription in HUVECs in a dose-dependent manner. In Western bolt

10 μ mol/L CPT decreased expression of β-catenin in nucleus of HUVECs (P<0.01). In immunohistochemistry

β-catenin was more potent in response to LiCl (an activator of the pathway) treatment. However

the signals were weaker in the nucleus of the CPT (10 μ mol/L) group

compared to the positive control. Also

VEGF and cyclin D1 were both eliminated by CPT in 5 and 10 μ mol/L doses (P<0.05). Our study supported the role of CPT as an angiogenic inhibitor

which may impact on the Wnt/β-catenin signaling pathway.

关键词

cryptotanshinoneangiogenesisWnt/β-catenin signaling pathwayHuman Umbilical Vein Endothelial Cellatherosclerosis

Keywords

cryptotanshinoneangiogenesisWnt/β-catenin signaling pathwayHuman Umbilical Vein Endothelial Cellatherosclerosis

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