Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats

Wen-zhan Tu; Rui-dong Cheng; Jie Hu; Jie-zhi Wang; Hai-yan Lin; En-miao Zou; Wan-sheng Wang; Xin-fa Lou; Song-he Jiang

doi:10.1007/s11655-014-1816-4

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Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats

OriginalPaper | Updated：2021-08-27

- Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats
- Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats
- 中国结合医学杂志（英文版） 2015年21卷第8期页码：610-617
- Affiliations：
  
  1. Department of Physical Medicine and Rehabilitation, the Second Affiliated Hospital of Wenzhou Medical College,Zhejiang Province,Wenzhou,China
  2. Department of Rehabilitation Medicine, Zhejiang Provincial People’s Hospital,Hangzhou,China
  3. Department of Anatomy, Wenzhou Medical College,Zhejiang Province,Wenzhou,China
- Author bio：
- Funds：
  
  Supported by a grant from Administration of Traditional Chinese Medicine of Zhejiang Province (No. 2009YB023)
- DOI：10.1007/s11655-014-1816-4
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-8-7，
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Tu, Wz., Cheng, Rd., Hu, J. et al. Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats., Chin. J. Integr. Med. 21, 610–617 (2015). https://doi.org/10.1007/s11655-014-1816-4

Wen-zhan Tu, Rui-dong Cheng, Jie Hu, et al. Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(8):610-617.
Tu, Wz., Cheng, Rd., Hu, J. et al. Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats., Chin. J. Integr. Med. 21, 610–617 (2015). https://doi.org/10.1007/s11655-014-1816-4 DOI：

Wen-zhan Tu, Rui-dong Cheng, Jie Hu, et al. Combination treatment with Gua Sha and Blood-letting causes attenuation of systemic inflammation, activated coagulation, tissue ischemia and injury during heatstroke in rats[J]. Chinese Journal of Integrative Medicine, 2015,21(8):610-617. DOI： 10.1007/s11655-014-1816-4.

摘要

Gua Sha and Blood-letting at the acupoints were Chinese traditional therapies for heatstroke. The purpose of present study was to assess the therapeutic effect of Gua Sha on the DU Meridian and Bladder Meridian combined with Blood-letting acupoints at Shixuan (EX-UE 11) and Weizhong (BL 40) on heatstroke. Anesthetized rats

immediately after the onset of heatstroke

were divided into four major groups: Gua Sha group

Blood-letting group

Gua Sha combined with Blood-letting group and model group. They were exposed to ambient temperature of 43 °C to induce heatstroke. Another group of rats were exposed to room temperature (26 °C) and used as normal control group. Their survival times were measured. In addition

their physiological and biochemical parameters were continuously monitored. When rats underwent heatstroke

their survival time values were found to be 21–25 min. Treatment of Gua Sha combined with Bloodletting greatly improved the survival time (230±22 min) during heatstroke. All heatstoke animals displayed and activated coagulation evidenced by increased prothrombin time (PT)

activated partial thromboplastin time (aPTT)

D-dimer

and decreased platelet count

protein C. Furthermore

the animals displayed systemic inflammation evidenced by increased the serum levels of cytokines interleukin-1ß (IL-1ß)

tumor necrosis factor α (TNF-α) and malondialdehyde (MDA). Biochemical markers evidenced by cellular ischemia and injury/dysfunction included increased plasma levels of blood urea nitrogen (BUN)

creatinine

serum glutamic oxaloacetic transaminase (SGOT)

serum glutamic pyruvic transaminase (SGPT)

and alkaline phosphatase (ALP) were all elevated during heatstroke. Core temperatures (Tco) were also increased during heatstroke. In contrast

the values of mean arterial pressure were signifificantly lower during heatstroke. These heatstroke reactions were all signifificantly suppressed by treatment of Gua Sha and Blood-letting

especially the combination therapy. Gua Sha combined with Blood-letting after heatstroke may improve survival by ameliorating systemic inflflammation

hypercoagulable state

and tissue ischemia and injury in multiple organs.

Abstract

immediately after the onset of heatstroke

were divided into four major groups: Gua Sha group

Blood-letting group

their physiological and biochemical parameters were continuously monitored. When rats underwent heatstroke

activated partial thromboplastin time (aPTT)

D-dimer

and decreased platelet count

protein C. Furthermore

the animals displayed systemic inflammation evidenced by increased the serum levels of cytokines interleukin-1ß (IL-1ß)

tumor necrosis factor α (TNF-α) and malondialdehyde (MDA). Biochemical markers evidenced by cellular ischemia and injury/dysfunction included increased plasma levels of blood urea nitrogen (BUN)

creatinine

serum glutamic oxaloacetic transaminase (SGOT)

serum glutamic pyruvic transaminase (SGPT)

and alkaline phosphatase (ALP) were all elevated during heatstroke. Core temperatures (Tco) were also increased during heatstroke. In contrast

the values of mean arterial pressure were signifificantly lower during heatstroke. These heatstroke reactions were all signifificantly suppressed by treatment of Gua Sha and Blood-letting

especially the combination therapy. Gua Sha combined with Blood-letting after heatstroke may improve survival by ameliorating systemic inflflammation

hypercoagulable state

and tissue ischemia and injury in multiple organs.

关键词

Gua ShaBlood-lettingheatstrokeischemiainflflammationcoagulationChinese Medicine

Keywords

Gua ShaBlood-lettingheatstrokeischemiainflflammationcoagulationChinese Medicine

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