Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells

Xiao-le Xu; Yan-juan Huang; Dan-yan Ling; Wei Zhang

doi:10.1007/s11655-014-1821-7

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Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells

OriginalPaper | Updated：2021-08-27

- Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells
- Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells
- 中国结合医学杂志（英文版） 2015年21卷第3期页码：204-210
- Affiliations：
  
  1. Department of Pharmacology, Nantong University Pharmacy College,Nantong,China
  2. Shanghai Health Vocational and Technical College,Shanghai,China
- Author bio：
- Funds：
  
  Supported by the Natural Science Foundation of Jiangsu Province (No. BK2009162) and the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (No. 09KJB310010) and the Priority Academic Program Development of Jiangsu Higher Education Institutions
- DOI：10.1007/s11655-014-1821-7
  中图分类号：
- 纸质出版日期：2015，
  
  网络出版日期：2014-7-30，
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Xu, Xl., Huang, Yj., Ling, Dy. et al. Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells., Chin. J. Integr. Med. 21, 204–210 (2015). https://doi.org/10.1007/s11655-014-1821-7

Xiao-le Xu, Yan-juan Huang, Dan-yan Ling, et al. Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells[J]. Chinese Journal of Integrative Medicine, 2015,21(3):204-210.
Xu, Xl., Huang, Yj., Ling, Dy. et al. Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells., Chin. J. Integr. Med. 21, 204–210 (2015). https://doi.org/10.1007/s11655-014-1821-7 DOI：

Xiao-le Xu, Yan-juan Huang, Dan-yan Ling, et al. Inhibitory effects of 2,3,4′,5-tetrahydroxystilbene-2-O-β-D-glucoside on angiotensin II-induced proliferation of vascular smooth muscle cells[J]. Chinese Journal of Integrative Medicine, 2015,21(3):204-210. DOI： 10.1007/s11655-014-1821-7.

摘要

To investigate the effect of 2

4′

5-tetrahydroxystilbene-2-O-β-D-glucoside (TSG)

an active component extracted from the root of Polygonum multiflorum

on angiotensin II (Ang II)-induced proliferation of cultured rat vascular smooth muscle cells (VSMCs) and to identify the potential mechanism. Cell proliferation and cell cycle were determined by cell counting

5-bromo-2′-deoxyuridine incorporation assay

proliferating cell nuclear antigen protein expression and flow cytometry. Levels of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2)

mitogenic extracellular kinase 1/2 (MEK1/2) and Src in VSMCs were measured by Western blot. The expression of c-fos

c-jun and c-myc mRNA were measured by reverse transcription polymerase chain reaction (RT-PCR). Intracellular reactive oxygen species (ROS) was measured by fluorescence assay. TSG significantly inhibited Ang II-induced VSMCs proliferation and arrested cells in the G /S checkpoint (P<0.05 or P<0.01). TSG decreased the levels of phosphorylated ERK1/2

MEK1/2 and Src in VSMCs (P<0.05 or P<0.01). TSG also suppressed c-fos

c-jun and c-myc mRNA expression <0.05 or P<0.01). In addition

the intracellular ROS was reduced by TSG (P<0.01). TSG inhibited Ang II-induced VSMCs proliferation. Its antiproliferative effect might be associated with down-regulation of intracellular ROS

followed by the suppression of the Src-MEK1/2-ERK1/2 signal pathway

and hence

blocking cell cycle progression.

Abstract

To investigate the effect of 2

4′

5-tetrahydroxystilbene-2-O-β-D-glucoside (TSG)

an active component extracted from the root of Polygonum multiflorum

5-bromo-2′-deoxyuridine incorporation assay

proliferating cell nuclear antigen protein expression and flow cytometry. Levels of phosphorylated extracellular signal-regulated kinase 1/2 (ERK1/2)

mitogenic extracellular kinase 1/2 (MEK1/2) and Src in VSMCs were measured by Western blot. The expression of c-fos

MEK1/2 and Src in VSMCs (P<0.05 or P<0.01). TSG also suppressed c-fos

c-jun and c-myc mRNA expression <0.05 or P<0.01). In addition

the intracellular ROS was reduced by TSG (P<0.01). TSG inhibited Ang II-induced VSMCs proliferation. Its antiproliferative effect might be associated with down-regulation of intracellular ROS

followed by the suppression of the Src-MEK1/2-ERK1/2 signal pathway

and hence

blocking cell cycle progression.

关键词

234′5-tetrahydroxystilbene-2-O-β-D-glucosidevascular smooth muscle cellsproliferationangiotensin IIChinese medicine Polygonum multiflorum

Keywords

234′5-tetrahydroxystilbene-2-O-β-D-glucosidevascular smooth muscle cellsproliferationangiotensin IIChinese medicine Polygonum multiflorum

references

Dzau VJ, Braun-Dullaeus RC, Sedding DG. Vascular proliferation and atherosclerosis: new perspectives and therapeutic strategies. Nat Med 2002;8:1249–1256.

Schmidt-Ott KM, Kagiyama S, Phillips MI. The multiple actions of angiotensin II in atherosclerosis. Regul Pept 2000;93:65–77.

Zheng SG, Qian ZY, Wen N, Xi L. Crocetin suppresses angiotensin II-induced vascular smooth-muscle cell proliferation through inhibition of ERK1/2 activation and cellcycle progression. J Cardiovasc Pharmacol 2007;50:519–525.

Irani K. Oxidant signaling in vascular cell growth, death, and survival: a review of the roles of reactive oxygen species in smooth muscle and endothelial cell mitogenic and apoptotic signaling. Circ Res 2000;87:179–183.

Zhou CH, Qian ZY, Zheng SG, Xiang M. ERK1/2 pathway is involved in the inhibitory effect of crocetin on angiotensin II-induced vascular smooth muscle cell proliferation. Eur J Pharmacol 2006;535:61–68.

Fang LH, Zhang YH, Ma JJ, Du GH, Ku BS, Yao HY, et al. Inhibitory effects of tetrandrine on the serum- and plateletderived growth factor-BB-induced proliferation of rat aortic smooth muscle cells through inhibition of cell cycle progression, DNA synthesis, ERK1/2 activation and c-fos expression. Atherosclerosis 2004;174:215–223.

Cui HH, Tian Y, Long SY. The effective mechanism of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in antiatherosclerosis. Prog Mod Biomed (Chin) 2009;9:3968–3971.

Liu QL, Xiao JH, Ma R, Ban Y, Wang JL. Effect of 2,3,5,4′-tetrahydroxystilbene-2-O-beta-D-glucoside on lipoprotein oxidation and proliferation of coronary arterial smooth cells. J Asian Nat Prod Res 2007;9:689–697.

Zhang W, Wang CH, Li F, Zhu WZ. 2,3,4′,5-tetrahydroxystilbene-2-O-beta-D-glucoside suppresses matrix metalloproteinase expression and inflammation in atherosclerotic rats. Clin Exp Pharmacol Physiol 2008;35:310–316.

Zhang W, Xu XL, Wang YQ, Wang CH, Zhu WZ. Effects of 2,3,4′,5-Tetrahydroxystilbene-2-O-β-D-glucoside on vascular endothelial dysfunction in atherogenic-diet rats. Planta Med 2009;75:1209–1214.

Chen L, Wang WY, Wang YP. Inhibitory effects of lithospermic acid on proliferation and migration of rat vascular smooth muscle cells. Acta Pharmacol Sin 2009;30:1245–1252.

Sun JJ, Kim HJ, Seo HG, Lee JH, Yun-Choi HS, Chang KC. YS 49, 1-(α-naphtylmethyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline, regulates angiotensin II-stimulated ROS production, JNK phosphorylation and vascular smooth muscle cell proliferation via the induction of heme oxygenase-1. Life Sci 2008;82:600–607.

Kastan MB, Bartek J. Cell-cycle checkpoints and cancer. Nature 2004;432:316–323.

Naryzhny SN. Proliferating cell nuclear antigen: a proteomics view. Cell Mol Life Sci 2008;65:3789–3808.

Duff JL, Marrero MB, Paxton WG, Schieffer B, Bernstein KE, Berk BC. Angiotensin II signal transduction and the mitogen-activated protein kinase pathway. Cardiovasc Res 1995;30:511–517.

Meloche S, Pouysségur J. The ERK1/2 mitogen-activated protein kinase pathway as a master regulator of the G1- to S-phase transition. Oncogene 2007;26:3227–3239.

Schaffer SW, Mozaffari MS. Angiotensin II signaling: cell growth and apoptosis. In Srivastava AK, Anand-Srivastava MB, eds. Signal transduction in the cardiovascular system in health and disease. New York: Springer; 2008:117–137.

Li L, Zhou Y, Wang C, Zhao YL, Zhang ZG, Fan D, et al. Src tyrosine kinase regulates angiotensin II-induced protein kinase Cζ activation and proliferation in vascular smooth muscle cells. Peptides 2010;31:1159–1164.

Yogi A, Callera GE, Montezano AC, Aranha AB, Tostes RC, Schiffrin EL, et al. Endothelin-1, but not Ang II, activates MAP kinases through c-Src independent Ras-Raf dependent pathways in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 2007;27:1960–1967.

Braun-Dullaeus RC, Mann MJ, Dzau VJ. Cell cycle progression: new therapeutic target for vascular proliferative disease. Circulation 1998;98:82–89.

Rocic P, Seshiah P, Griendling KK. Reactive oxygen species sensitivity of angiotensin II-dependent translation initiation in vascular smooth muscle cells. J Biol Chem 2003;278:36973–36979.

Hong HJ, Chan P, Liu JC, Juan SH, Huang MT, Lin JG, et al. Angiotensin II induces endothelin-1 gene expression via extracellular signal-regulated kinase pathway in rat aortic smooth muscle cells. Cardiovasc Res 2004;61:159–168.

Chao HH, Juan SH, Liu JC, Yang HY, Yang E, Cheng TH, et al. Resveratrol inhibits angiotensin II-induced endothelin-1 gene expression and subsequent proliferation in rat aortic smooth muscle cells. Eur J Pharmacol 2005;515:1–9.

Li PG, Xu JW, Ikeda K, Kobayakawa A, Kayano Y, Mitani T, et al. Caffeic acid inhibits vascular smooth muscle cell proliferation induced by angiotensin II in stroke-prone spontaneously hypertensive rats. Hypertens Res 2005;28:369–377.

Lv LS, Gu XH, Tang J, Ho CT. Antioxidant activity of stilbene glycoside from Polygonum multiflorum Thunb in vivo. Foodchem 2007;104:1678–1681.

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