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Anticancer effect of total annonaceous acetogenins on hepatocarcinoma
Anticancer effect of total annonaceous acetogenins on hepatocarcinoma
- 中国结合医学杂志（英文版） 2015年21卷第9期 页码：682-688
- Affiliations：
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing,China
2. Henan Institute of Engineering,Zhengzhou,China
- Author bio：
- Funds：Supported by National Mega-project for Innovative Drugs (No. 2012ZX09301002-001-026 and 2009ZX09103-390) and Beijing Natural Science Foundation (No. 7122112)
DOI：10.1007/s11655-014-1845-z
中图分类号：纸质出版日期：2015，
网络出版日期：2014-6-18，
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Yang, Rm., Li, Wm., Hu, Wj. et al. Anticancer effect of total annonaceous acetogenins on hepatocarcinoma., Chin. J. Integr. Med. 21, 682–688 (2015). https://doi.org/10.1007/s11655-014-1845-z
Run-mei Yang, Wen-min Li, Wei-jun Hu, et al. Anticancer effect of total annonaceous acetogenins on hepatocarcinoma[J]. Chinese Journal of Integrative Medicine, 2015,21(9):682-688.
Yang, Rm., Li, Wm., Hu, Wj. et al. Anticancer effect of total annonaceous acetogenins on hepatocarcinoma., Chin. J. Integr. Med. 21, 682–688 (2015). https://doi.org/10.1007/s11655-014-1845-z DOI：
Run-mei Yang, Wen-min Li, Wei-jun Hu, et al. Anticancer effect of total annonaceous acetogenins on hepatocarcinoma[J]. Chinese Journal of Integrative Medicine, 2015,21(9):682-688. DOI： 10.1007/s11655-014-1845-z.
摘要
To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma. The inhibitory effect of TAAs was demonstrated in H22-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis. The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 µg/mL (15.13–26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs. TAAs is effective for hepatocarcinoma
via inhibiting proliferation and inducing apoptosis.
Abstract
To confirm the anticancer effect of total annonaceous acetogenins (TAAs) abstracted from Annona squamosa Linn. on human hepatocarcinoma. The inhibitory effect of TAAs was demonstrated in H22-bearing mice. The potency of TAAs was confirmed as its 50% inhibiting concentration (IC50) on Bel-7402 cell under Sulfur Rhodamine B staining. Both underlying mechanisms were explored as cellular apoptosis and cell cycle under flow cytometry. Mitochondrial and recipient apoptotic pathways were differentiated as mitochondrial membrane potential under flow cytometry and caspases activities under fluorescence analysis. The inhibitory rate of TAAs in mice was 50.98% at 4 mg/kg dose. The IC50 of TAAs on Bel-7402 was 20.06 µg/mL (15.13–26.61µg/mL). Effective mechanisms of TAAs were confirmed as both of arresting cell cycle at G1 phase and inducing apoptosis dose- and time-dependently. Mitochondrial and recipient pathways involved in apoptotic actions of TAAs. TAAs is effective for hepatocarcinoma
via inhibiting proliferation and inducing apoptosis.
关键词
annonaceous acetogeninshepatocarcinomaproliferationapoptosis
Keywords
annonaceous acetogeninshepatocarcinomaproliferationapoptosis
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