Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells
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OriginalPaper|Updated:2021-08-27
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Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells
Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells
中国结合医学杂志(英文版)2015年21卷第9期 页码:676-681
Affiliations:
School of Stomatology, Lanzhou University,Lanzhou,China
Author bio:
Funds:
Supported by the National Natural Science Foundation of China (No. 81102712) and the Traditional Chinese Medicine Foundation of Gansu Province, China (No. GZK-2012-47)
Yin, Lh., Cheng, Wx., Qin, Zs. et al. Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells., Chin. J. Integr. Med. 21, 676–681 (2015). https://doi.org/10.1007/s11655-014-1856-9
Li-hua Yin, Wen-xiao Cheng, Zi-shun Qin, et al. Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells[J]. Chinese Journal of Integrative Medicine, 2015,21(9):676-681.
Yin, Lh., Cheng, Wx., Qin, Zs. et al. Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells., Chin. J. Integr. Med. 21, 676–681 (2015). https://doi.org/10.1007/s11655-014-1856-9DOI:
Li-hua Yin, Wen-xiao Cheng, Zi-shun Qin, et al. Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells[J]. Chinese Journal of Integrative Medicine, 2015,21(9):676-681. DOI: 10.1007/s11655-014-1856-9.
Effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells
摘要
To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration. To determine the optimum concentration
the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4
5)-dimethylthiahiazo(-z-y1)-3
5-di-phenytetrazoliumromide
alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2
collagen alpha-2(I) chain
osteopontin
osteocalcin protein were examined using real-time polymerase chain reaction. Compared with the control group
a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However
concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control. Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.
Abstract
To evaluate the effects of ginsenoside Rg-1 on the proliferation and osteogenic differentiation of human periodontal ligament stem cells (hPDLSCs) and to explore the possible application on the alveolar bone regeneration. To determine the optimum concentration
the effects of ginsenoside Rg-1 ranging from 10 to 100 μmol/L were evaluated by 3-(4
5)-dimethylthiahiazo(-z-y1)-3
5-di-phenytetrazoliumromide
alkaline phosphatase activity and calcium deposition. Expressions of runt-related transcription factor 2
collagen alpha-2(I) chain
osteopontin
osteocalcin protein were examined using real-time polymerase chain reaction. Compared with the control group
a certain concentration (10 μmol/L) of the Rg-1 solution significantly enhanced the proliferation and osteogenic differentiation of hPDLSCs (P<0.05). However
concentrations that exceeds 100 μmol/L led to cytotoxicity whereas concentrations below 10 nmol/L showed no significant effect as compared with the control. Ginsenoside Rg-1 can enhance the proliferation and osteogenic differentiation of hPDLSCs at an optimal concentration of 10 μmol/L.
关键词
ginsenoside Rg-1osteogenic differentiationproliferationhuman periodontal ligament stem cellChinese Medicine
Keywords
ginsenoside Rg-1osteogenic differentiationproliferationhuman periodontal ligament stem cellChinese Medicine
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