Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway

Bin Yu; Cong-qi Dai; Zhen-you Jiang; En-qing Li; Chen Chen; Xian-lin Wu; Jia Chen; Qian Liu; Chang-lin Zhao; Jin-xiong He; Da-hong Ju; Xiao-yin Chen

doi:10.1007/s11655-014-1860-0

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Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway

OriginalPaper | Updated：2021-08-27

- Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway
- Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway
- 中国结合医学杂志（英文版） 2014年20卷第7期页码：540-545
- Affiliations：
  
  1. Department of Traditional Chinese Medicine, School of Medicine, Jinan University,Guangzhou,China
  2. Shanghai Cancer Center and Shanghai Medical College, Fudan University,Shanghai,China
  3. Department of Microbiology and Immunology, School of Medicine, Jinan University,Guangzhou,China
  4. Shandong Provincial Corps Hospital of Chinese People’s Armed Police Forces,Jinan,China
  5. Department of Obstetrics and Gynecology, First Affiliated Hospital of Jinan University,Guangzhou,China
  6. Department of Gynaecology and Obstetrics, Shenzhen Baoan Maternal and Child Health Hospital,Shenzhen,China
  7. Department of Oncology, Clifford Hospital of Guangzhou University of Traditional Chinese Medicine,Guangzhou,China
  8. Institute of Basic Theories of Traditional Chinese Medicine, China Academy of Traditional Chinese Medicine,Beijing,China
- Author bio：
- Funds：
  
  Supported by the National Natural Science Foundation of China (No. 81273616 and 30973693), the Doctoral Fund of the Ministry of Education 2010 (No. 20104401110003) and the Natural Science Foundation of Guangdong Province (No. S2013010013434);The first two authors contributed equally to this work
- DOI：10.1007/s11655-014-1860-0
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-6-28，
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Yu, B., Dai, Cq., Jiang, Zy. et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway., Chin. J. Integr. Med. 20, 540–545 (2014). https://doi.org/10.1007/s11655-014-1860-0

Bin Yu, Cong-qi Dai, Zhen-you Jiang, et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(7):540-545.
Yu, B., Dai, Cq., Jiang, Zy. et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway., Chin. J. Integr. Med. 20, 540–545 (2014). https://doi.org/10.1007/s11655-014-1860-0 DOI：

Bin Yu, Cong-qi Dai, Zhen-you Jiang, et al. Andrographolide as an Anti-H1N1 drug and the mechanism related to retinoic acid-inducible gene-I-like receptors signaling pathway[J]. Chinese Journal of Integrative Medicine, 2014,20(7):540-545. DOI： 10.1007/s11655-014-1860-0.

摘要

To observe the anti-virus effects of andrographolide (AD) on the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs) signaling pathway when immunological cells were infected with H1N1. Leukomonocyte was obtained from umbilical cord blood by Ficoll density gradient centrifugation

and immunological cells were harvested after cytokines stimulation. Virus infected cell model was established by H1N1 co-cultured with normal human bronchial epithelial cell line (16HBE). The optimal concentration of AD was defined by methyl-thiazolyl-tetrazolium (MTT) assay. After the virus infected cell model was established

AD was added into the medium as a treatment intervention. After 24-h co-culture

cell supernatant was collected for interferon gamma (IFN-γ) and interleukin-4 (IL-4) enzyme-linked immunosorbent assay (ELISA) detection while immunological cells for real-time polymerase chain reaction (RT-PCR). The optimal concentration of AD for anti-virus effect was 250 μg/mL. IL-4 and IFN-γ in the supernatant and mRNA levels in RLRs pathway increased when cells was infected by virus

RIG-I

IFN-β promoter stimulator-1 (IPS-1)

interferon regulatory factor (IRF)-7

IRF-3 and nuclear transcription factor κB (NF-κB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium

the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased

RIG-I

IPS-1

IRF-7

IRF-3 and NF-κB decreased significantly in each group with significant statistic differences (P<0.05). The RLRs mediated viral recognition provided a potential molecular target for acute viral infections and andrographolide could ameliorate H1N1 virus-induced cell mortality. And the antiviral effects might be related to its inhibition of viral-induced activation of the RLRs signaling pathway.

Abstract

AD was added into the medium as a treatment intervention. After 24-h co-culture

RIG-I

IFN-β promoter stimulator-1 (IPS-1)

interferon regulatory factor (IRF)-7

IRF-3 and nuclear transcription factor κB (NF-κB) mRNA levels increased significantly (P<0.05). When AD was added into co-culture medium

the levels of IL-4 and IFN-γ were lower than those in the non-interference groups and the mRNA expression levels decreased

RIG-I

IPS-1

IRF-7

关键词

andrographolideH1N1dendritic cellsretinoic acid-inducible gene-Iinterferon-β promoter stimulator-1interferon regulatory factornuclear transcription factor κB

Keywords

andrographolideH1N1dendritic cellsretinoic acid-inducible gene-Iinterferon-β promoter stimulator-1interferon regulatory factornuclear transcription factor κB

references

Schnell JR, Chou JJ. Structure and mechanism of the M2 proton channel of influenza A virus. Nature 2008;451:591–595.

Jefferson T, Demicheli V, Di Pietrantonj C, Rivetti D. Amantadine and rimantadine for influenza A in adults. Cochrane Database Syst Rev 2006;2:CD001169.

Moscona A. Global transmission of oseltamivir-resistant influenza. N Engl J Med 2009;360:953–956.

Cameron CE, Castro C. The mechanism of action of ribavirin: lethal mutagenesis of RNA virus genomes mediated by the viral RNA-dependent RNA polymerase. Curr Opin Infect Dis 2001;14:757.

Baumgarth N, Herman OC, Jager GC, Brown L, Herzenberg LA. Innate and acquired humoral immunities to influenza virus are mediated by distinct arms of the immune system. Proc Nation Acad Sci 1999;96:2250.

Takeuchi O, Akira S. MDA5/RIG-I and virus recognition. Curr Opin Immunol 2008;20:17–22.

Frucht DM, Fukao T, Bogdan C, Schindler H, O’Shea JJ, Koyasu S. IFN-gamma production by antigen-presenting cells: mechanisms emerge. Trends Immunol 2001;22:556–560.

Sen GC. Viruses and interferons. Annu Rev Microbiol 2001;55:255–281.

Chen Z, Yu B, Wu XL, Dai CQ, Qian GQ, Yu JZ, et al. Carboxymethylpachymaran enhances immunologic function of dendritic cells cultured in two kinds of hepatoma carcinoma cell line’s supernatant via nuclear factor κB/Rel pathway. Chin J Integr Med 2012;18:203–208.

Seubsasana S, Pientong C, Ekalaksananan T, Thongchai S, Aromdee C. A potential andrographolide analogue against the replication of herpes simplex virus type 1 in vero cells. Med Chem 2011;7:237–244.

Chen C, Jiang ZY, Yu B, Wu XL, Dai CQ, Zhao CL, et al. Study on the anti-H1N1 virus effects of quercetin and oseltamivir and their mechanism related to TLR7 pathway. J Asian Nat Prod Res 2012;14:877–885.

Lu CJ, Luo Y, Zhou H, Qin XL, Chen BJ, Tang XC, et al. A preliminary study on the medical expenditure of Chinese medicine and integrative medicine treatment for influenza a (H1N1) in the fever clinics. Chin J Integr Med 2010;16:493–497.

Sui J, Hwang WC, Perez S, Wei G, Aird D, Chen LM, et al. Structural and functional bases for broad-spectrum neutralization of avian and human influenza A viruses. Nature Struct Molecul Biol 2009;16:265–273.

Govorkova EA. Consequences of resistance: in vitro fitness, in vivo infectivity, and transmissibility of oseltamivirresistant influenza A viruses. Influenza Other Respirat Virus 2013;7:50–57.

Chao DL, Bloom JD, Kochin BF, Antia R, Longini IM. The global spread of drug-resistant influenza. J Roy Soc Interf 2012;9:648–656.

Seibert CW, Rahmat S, Krammer F, Palese P, Bouvier NM. Efficient transmission of pandemic H1N1 influenza viruses with high-level oseltamivir resistance. J Virol 2012;86:5386–5389.

Ghedin E, Holmes EC, DePasse JV, Pinilla LT, Fitch A, Hamelin M-E, et al. Presence of oseltamivir-resistant pandemic A/H1N1 minor variants before drug therapy with subsequent selection and transmission. J Infect Dis 2012;206:1504–1511.

Hong ML, Song Y, Li XM. Effects and mechanisms of actions of Chinese herbal medicines for asthma. Chin J Integr Med 2011;17:483–491.

Kawai T, Takahashi K, Sato S, Coban C, Kumar H, Kato H, et al. IPS-1, an adaptor triggering RIG-I-and Mda5-mediated type I interferon induction. Nature immunol 2005;6:981–988.

Takahashi K, Kawai T, Kumar H, Sato S, Yonehara S, Akira S. Cutting edge: roles of caspase-8 and caspase-10 in innate immune responses to double-stranded RNA. J Immunol 2006;176:4520–4524.

Lim JC, Chan TK, Ng DS, Sagineedu SR, Stanslas J, Wong WS. Andrographolide and its analogues: versatile bioactive molecules for combating inflammation and cancer. Clin Exp Pharmacol Physiol 2012;39:300–310.

Moore CB, Ting JP. Regulation of mitochondrial antiviral signaling pathways. Immunity 2008;28:735–739.

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