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Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats
Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats
- 中国结合医学杂志（英文版） 2014年20卷第7期 页码：524-533
- Affiliations：
1. Department of Cardiology, Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan,China
2. Shandong University of Traditional Chinese Medicine,Jinan,China
- Author bio：
- Funds：Supported by the National Natural Science Foundation of China (No. 81072962) and the Special Fund of Taishan Scholars Program of Shandong Province (No. 2012-55)
DOI：10.1007/s11655-014-1861-z
中图分类号：纸质出版日期：2014，
网络出版日期：2014-6-28，
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Li, X., Jiang, Yh., Jiang, P. et al. Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats., Chin. J. Integr. Med. 20, 524–533 (2014). https://doi.org/10.1007/s11655-014-1861-z
Xiao Li, Yue-hua Jiang, Ping Jiang, et al. Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats[J]. Chinese Journal of Integrative Medicine, 2014,20(7):524-533.
Li, X., Jiang, Yh., Jiang, P. et al. Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats., Chin. J. Integr. Med. 20, 524–533 (2014). https://doi.org/10.1007/s11655-014-1861-z DOI：
Xiao Li, Yue-hua Jiang, Ping Jiang, et al. Effect of Guizhi Decoction (桂枝汤) on heart rate variability and regulation of cardiac autonomic nervous imbalance in diabetes mellitus rats[J]. Chinese Journal of Integrative Medicine, 2014,20(7):524-533. DOI： 10.1007/s11655-014-1861-z.
摘要
To observe abnormalities in heart rate variability (HRV) in diabetic rats and to explore the effects of treatment with Guizhi Decoction (桂枝汤) on cardiac autonomic nervous (CAN) imbalance. A radio-telemetry system for monitoring physiological parameters was implanted into rats to record electrocardiac signals and all indictors of HRV [time domain measures: standard deviation of all RR intervals in 24 h (SDNN)
root mean square of successive differences (RMSSD)
percentage of differences between adjacent RR intervals greater than 50 ms (PNN50)
and standard deviation of the averages of RR intervals (SDANN); frequency domain measures: low frequency (LF)
high frequency (HF)
total power (TP)
and LF/HF ratio]. The normal group was randomly selected
and the remaining rats were used to establish streptozocin (STZ)-induced diabetic model. After 4 weeks
the model rats were divided into the model group
the methycobal group
and the Guizhi Decoction group
9 rats in each group. Four weeks after intragastric administration of the corresponding drugs
the right atria of the rats were collected for immunohistochemical staining of tyrosine hydroxylase (TH) and choline acetyltransferase (CHAT) to observe the distribution of the sympathetic and vagus nerves in the right atrium. The myocardial homogenate from the interventricular septum and the left ventricle was used for determination of TH
CHAT
growth-associated protein 43 (GAP-43)
nerve growth factor (NGF)
and ciliary neurotrophic factor (CNTF) levels using an enzyme-linked immunosorbent assay. (1) STZ rats had elevated blood glucose levels
reduced body weight
and decreased heart rate; there was no difference between the model group and the drug treated groups. (2) Compared with the model group
only RMSSD and TP increased in the methycobal group significantly (P<0.05); SDNN
RMSSD
PNN50
LF
HF
and TP increased
LF/HF decreased (P<0.05)
and SDANN just showed a decreasing trend in the Guizhi Decoction group (P>0.05). TH increased
CHAT decreased
and TH/CHAT increased in the myocardial homogenate of the model group (P<0.05). Compared with the model group
left ventricular TH reduced in the methycobal group; and in the Guizhi Decoction group CHAT increased
while TH and TH/CHAT decreased (P<0.05). Compared with the model group
CNTF in the interventricular septum increased in the methycobal group (P<0.05); GAP-43 increased
NGF decreased
and CNTF increased (P<0.05) in the Guizhi Decoction group. There were significant differences in the reduction of NGF and elevation of CNTF between the Guizhi Decoction group and the methycobal group (P<0.05). (3) Immunohistochemical results showed that TH expression significantly increased and CHAT expression significantly decreased in the myocardia of the model group
whereas TH expression decreased and CHAT expression increased in the Guizhi Decoction group (P<0.05). Guizhi Decoction was effective in improving the function of the vagus nerve
and it could alleviate autonomic nerve damage.
Abstract
To observe abnormalities in heart rate variability (HRV) in diabetic rats and to explore the effects of treatment with Guizhi Decoction (桂枝汤) on cardiac autonomic nervous (CAN) imbalance. A radio-telemetry system for monitoring physiological parameters was implanted into rats to record electrocardiac signals and all indictors of HRV [time domain measures: standard deviation of all RR intervals in 24 h (SDNN)
root mean square of successive differences (RMSSD)
percentage of differences between adjacent RR intervals greater than 50 ms (PNN50)
and standard deviation of the averages of RR intervals (SDANN); frequency domain measures: low frequency (LF)
high frequency (HF)
total power (TP)
and LF/HF ratio]. The normal group was randomly selected
and the remaining rats were used to establish streptozocin (STZ)-induced diabetic model. After 4 weeks
the model rats were divided into the model group
the methycobal group
and the Guizhi Decoction group
9 rats in each group. Four weeks after intragastric administration of the corresponding drugs
the right atria of the rats were collected for immunohistochemical staining of tyrosine hydroxylase (TH) and choline acetyltransferase (CHAT) to observe the distribution of the sympathetic and vagus nerves in the right atrium. The myocardial homogenate from the interventricular septum and the left ventricle was used for determination of TH
CHAT
growth-associated protein 43 (GAP-43)
nerve growth factor (NGF)
and ciliary neurotrophic factor (CNTF) levels using an enzyme-linked immunosorbent assay. (1) STZ rats had elevated blood glucose levels
reduced body weight
and decreased heart rate; there was no difference between the model group and the drug treated groups. (2) Compared with the model group
only RMSSD and TP increased in the methycobal group significantly (P<0.05); SDNN
RMSSD
PNN50
LF
HF
and TP increased
LF/HF decreased (P<0.05)
and SDANN just showed a decreasing trend in the Guizhi Decoction group (P>0.05). TH increased
CHAT decreased
and TH/CHAT increased in the myocardial homogenate of the model group (P<0.05). Compared with the model group
left ventricular TH reduced in the methycobal group; and in the Guizhi Decoction group CHAT increased
while TH and TH/CHAT decreased (P<0.05). Compared with the model group
CNTF in the interventricular septum increased in the methycobal group (P<0.05); GAP-43 increased
NGF decreased
and CNTF increased (P<0.05) in the Guizhi Decoction group. There were significant differences in the reduction of NGF and elevation of CNTF between the Guizhi Decoction group and the methycobal group (P<0.05). (3) Immunohistochemical results showed that TH expression significantly increased and CHAT expression significantly decreased in the myocardia of the model group
whereas TH expression decreased and CHAT expression increased in the Guizhi Decoction group (P<0.05). Guizhi Decoction was effective in improving the function of the vagus nerve
and it could alleviate autonomic nerve damage.
关键词
diabetes mellituscardiac autonomic neuropathysympathetic-vagal imbalanceHeart Rate VariabilityChinese MedicineGuizhi Decoction
Keywords
diabetes mellituscardiac autonomic neuropathysympathetic-vagal imbalanceHeart Rate VariabilityChinese MedicineGuizhi Decoction
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