Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats

Zheng-miao Yu; Zhi-hui Liu; Jing Chen; Qiang Zeng

doi:10.1007/s11655-014-1863-x

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Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats

OriginalPaper | Updated：2021-08-27

- Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats
- Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats
- 中国结合医学杂志（英文版） 2014年20卷第12期页码：934-943
- Affiliations：
  
  1. Department of Cerebropathia, the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
  2. Department of Clinical Laboratory, the First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,China
  3. Hospital of Guangdong University of Technology,Guangzhou,China
  4. Library of Guangzhou University of Chinese Medicine,Guangzhou,China
- Author bio：
- Funds：
  
  Supported by National Natural Science Foundation of China (No. 30801468)
- DOI：10.1007/s11655-014-1863-x
  中图分类号：
- 纸质出版日期：2014，
  
  网络出版日期：2014-11-27，
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Yu, Zm., Liu, Zh., Chen, J. et al. Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats., Chin. J. Integr. Med. 20, 934–943 (2014). https://doi.org/10.1007/s11655-014-1863-x

Zheng-miao Yu, Zhi-hui Liu, Jing Chen, et al. Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(12):934-943.
Yu, Zm., Liu, Zh., Chen, J. et al. Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats., Chin. J. Integr. Med. 20, 934–943 (2014). https://doi.org/10.1007/s11655-014-1863-x DOI：

Zheng-miao Yu, Zhi-hui Liu, Jing Chen, et al. Anti-inflammatory effect of Qingwen Baidu Decoction (清瘟败毒饮) in sepsis rats[J]. Chinese Journal of Integrative Medicine, 2014,20(12):934-943. DOI： 10.1007/s11655-014-1863-x.

摘要

To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction (清瘟败毒饮

QBD) from the view of holistic biology. The rats were randomly divided into a normal conrol group

a lipopolysaccharide (LPS) group

the low- and high-dose QBD groups

and a dexamethasone (DXM) group. NR8383 cells were treated with culture fluid containing 6% serum from rats of each group respectively. Inflammatory mediators were detected by reverse transcription polymerase chain reaction (RT-PCR)

Western blotting hybridization

enzyme linked immunosorbent assay (ELISA)

polymerase chain reaction (PCR) gene array and antibody array. It is showed that the levels of interleukin (IL)-1α

IL-4 and IL-12 were enhanced in the low-dose QBD group; levels of IL-1α

IL-12 and IL-18 were augmented in the high-dose QBD group

compared with the LPS group after ELISA detection. Western blot showed that IL-1β and tumor necrosis factor (TNF)-α expression of the control group were lower than other groups. IL-1β level of the low-dose and high-dose QBD groups detected by RT-PCR was higher in early stage but lower after 24 h than that of the control group (P<0.01). Expression of 84 main inflammatory cytokines and receptors was detected by rat inflammatory cytokines and receptors PCR array. Up-regulation genes were 22 in both the LPS group and the low-dose QBD group

among which 16 up-regulating genes were the same. In these 16 genes

the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group

4 were similar to and 3 were more. Twenty-nine main cytokines were inspected by rat cytokine antibody array. Intergroup gray value differences were found in 7 expressed cytokines. The levels of these 7 cytokines in the lowdose QBD group were all lower than those in the the LPS group. QBD has anti-inflammatory effect on sepsis by changing the level of inflammatory mediators.

Abstract

To explore the pharmacological anti-inflammatory mechanism of Chinese formula Qingwen Baidu Decoction (清瘟败毒饮

QBD) from the view of holistic biology. The rats were randomly divided into a normal conrol group

a lipopolysaccharide (LPS) group

the low- and high-dose QBD groups

Western blotting hybridization

enzyme linked immunosorbent assay (ELISA)

polymerase chain reaction (PCR) gene array and antibody array. It is showed that the levels of interleukin (IL)-1α

IL-4 and IL-12 were enhanced in the low-dose QBD group; levels of IL-1α

IL-12 and IL-18 were augmented in the high-dose QBD group

among which 16 up-regulating genes were the same. In these 16 genes

the up-regulating amplitude of 9 genes in the low-dose QBD group was less than that in the LPS group

关键词

sepsissummer heat syndromeQingwen Baidu Decoctioncytokinemediator networkChinese Medicine

Keywords

sepsissummer heat syndromeQingwen Baidu Decoctioncytokinemediator networkChinese Medicine

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